Restriction map for pF1KB7230
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BcnI        BfrI        BglI        
BlnI        BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MluI        MroI        MspA1I      MspI        MvnI        
MwoI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        TaqI        Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AfaI          981  GTAC                 GT^AC
AflIII        925  ACATGT               A^C[GA][CT]GT
AlwNI         594  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         971  GAGCAC               G[AT]GC[AT]^C
BbsI          535  GTCTTC               GAAGAC(2/6)
BciVI         703  GTATCC               GTATCC(6/5)
BclI          302  TGATCA               T^GATCA
BfaI          991  CTAG                 C^TAG
BlpI          375  GCTTAGC              GC^T[ACGT]AGC
BmyI          971  GAGCAC               G[AGT]GC[ACT]^C
BpmI          512  CTGGAG               CTGGAG(16/14)
Bpu1102I      375  GCTTAGC              GC^T[ACGT]AGC
BpuAI         535  GTCTTC               GAAGAC(2/6)
BsiHKAI       971  GAGCAC               G[AT]GC[AT]^C
BsmAI           7  GTCTC                GTCTC(1/5)
Bsp1286I      971  GAGCAC               G[AGT]GC[ACT]^C
BspLU11I      925  ACATGT               A^CATGT
BspMI         646  GCAGGT               ACCTGC(4/8)
CelII         375  GCTTAGC              GC^T[ACGT]AGC
DsaI          251  CCATGG               C^C[GA][CT]GG
EcoT14I       251  CCATGG               C^C[AT][AT]GG
FbaI          302  TGATCA               T^GATCA
HinfI         278  GATTC                G^A[ACGT]TC
HphI          281  TCACC                GGTGA(8/7)
MaeI          991  CTAG                 C^TAG
MaeIII        437  GTTAC                ^GT[ACGT]AC
MfeI           69  CAATTG               C^AATTG
MunI           69  CAATTG               C^AATTG
NcoI          251  CCATGG               C^CATGG
RsaI          981  GTAC                 GT^AC
SfcI          878  CTGTAG               C^T[GA][CT]AG
SspI          171  AATATT               AAT^ATT
StuI          545  AGGCCT               AGG^CCT
StyI          251  CCATGG               C^C[AT][AT]GG
TfiI          278  GATTC                G^A[AT]TC
XcmI          632  CCACCTTGTTTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AvaII         507  GGTCC                G^G[AT]CC
              629  GGACC               
BalI          254  TGGCCA               TGG^CCA
              410  TGGCCA              
BamHI         154  GGATCC               G^GATCC
              852  GGATCC              
BbvI          216  GCAGC                GCAGC(8/12)
              778  GCTGC               
BglII          91  AGATCT               A^GATCT
              112  AGATCT              
BsiYI         246  CCTCTCCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              896  CCTTGACATGG         
BslI          246  CCTCTCCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              896  CCTTGACATGG         
BsmFI         628  GGGAC                GGGAC(10/14)
              508  GTCCC               
BstF5I        243  CATCC                GGATG(2/0)
              386  CATCC               
Cfr13I        507  GGTCC                G^G[ACGT]CC
              629  GGACC               
EaeI          254  TGGCCA               [CT]^GGCC[GA]
              410  TGGCCA              
Fnu4HI        216  GCAGC                GC^[ACGT]GC
              778  GCTGC               
FokI          243  CATCC                GGATG(9/13)
              386  CATCC               
ItaI          216  GCAGC                GC^[ACGT]GC
              778  GCTGC               
MluNI         254  TGGCCA               TGG^CCA
              410  TGGCCA              
MscI          254  TGGCCA               TGG^CCA
              410  TGGCCA              
MslI          282  CACCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              715  CATGCTCATG          
NspI          714  ACATGC               [GA]CATG^[CT]
              925  ACATGT              
Sau96I        507  GGTCC                G^G[ACGT]CC
              629  GGACC               
SfaNI         372  GATGC                GCATC(5/9)
              892  GATGC               
TseI          216  GCAGC                G^C[AT]GC
              778  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BsaJI       
BsrI        BssKI       BstNI       BstYI       DdeI        
DpnII       EcoRII      HaeIII      MboI        MboII       
MflI        MnlI        MseI        MvaI        NdeII       
NlaIII      NlaIV       Sau3AI      ScrFI       Tru9I       
Tsp509I     TspRI       XhoII       
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