Restriction map for
pF1KB8653
(Circular) (Six-base) MAPSORT of: pF1KB8653.seq Check: 7745 from: 1 to: 3815
pF1KB8653 3815 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 358 358
Size: 3815
AccI GT'mk_AC
Cuts at: 853 853
Size: 3815
Acc65I G'GTAC_C
Cuts at: 620 831 620
Size: 211 3604
AclI AA'CG_TT
Cuts at: 3655 3655
Size: 3815
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1640 2072 2819 3087 1640
Size: 432 747 268 2368
Fragments arranged by size:
2368 747 432 268
AflIII A'CryG_T
Cuts at: 374 1167 2271 3248 374
Size: 793 1104 977 941
Fragments arranged by size:
1104 977 941 793
AgeI A'CCGG_T
Cuts at: 2203 2203
Size: 3815
AlwNI CAG_nnn'CTG
Cuts at: 1120 2687 1120
Size: 1567 2248
ApaLI G'TGCA_C
Cuts at: 2585 2585
Size: 3815
ApoI r'AATT_y
Cuts at: 819 3168 3424 819
Size: 2349 256 1210
Fragments arranged by size:
2349 1210 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3815
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
AvaI C'yCGr_G
Cuts at: 548 835 548
Size: 287 3528
BamHI G'GATC_C
Cuts at: 726 840 726
Size: 114 3701
BanI G'GyrC_C
Cuts at: 145 620 631 745 831 1245 1493 1528 145
Size: 475 11 114 86 414 248 35 2432
Fragments arranged by size:
2432 475 414 248 114 86 35 11
BanII G_rGCy'C
Cuts at: 713 785 829 1859 713
Size: 72 44 1030 2669
Fragments arranged by size:
2669 1030 72 44
BbeI G_GCGC'C
Cuts at: 149 635 1249 1497 149
Size: 486 614 248 2467
Fragments arranged by size:
2467 614 486 248
BciVI GTATCCnnnnn_n'
Cuts at: 1713 2474 3815 1713
Size: 761 1341 1713
Fragments arranged by size:
1713 1341 761
BclI T'GATC_A
Cuts at: 1335 1335
Size: 3815
BglI GCCn_nnn'nGGC
Cuts at: 451 640 451
Size: 189 3626
BglII A'GATC_T
Cuts at: 1330 3367 1330
Size: 2037 1778
BlpI GC'TnA_GC
Cuts at: 927 927
Size: 3815
Bme1580I G_kGCm'C
Cuts at: 1440 1533 2589 1440
Size: 93 1056 2666
Fragments arranged by size:
2666 1056 93
BmgBI CAC'GTC
Cuts at: 375 375
Size: 3815
BmrI ACTGGGnnnn_n'
Cuts at: 728 1027 1108 1443 3587 728
Size: 299 81 335 2144 956
Fragments arranged by size:
2144 956 335 299 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 3815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 666 666
Size: 3815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 1870 2362 2660 991
Size: 879 492 298 2146
Fragments arranged by size:
2146 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 507 3519 507
Size: 3012 803
BsaAI yAC'GTr
Cuts at: 705 1168 705
Size: 463 3352
BsaBI GATnn'nnATC
Cuts at: 6 1329 3366 6
Size: 1323 2037 455
Fragments arranged by size:
2037 1323 455
BsaHI Gr'CG_yC
Cuts at: 146 632 1246 1494 2196 3692 146
Size: 486 614 248 702 1496 269
Fragments arranged by size:
1496 702 614 486 269 248
BsaWI w'CCGG_w
Cuts at: 1004 1525 2203 2477 2624 3035 1004
Size: 521 678 274 147 411 1784
Fragments arranged by size:
1784 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 247 277 247
Size: 30 3785
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 289
Size: 3815
BseYI C'CCAG_C
Cuts at: 670 1240 1998 2575 3071 670
Size: 570 758 577 496 1414
Fragments arranged by size:
1414 758 577 570 496
BsiEI CG_ry'CG
Cuts at: 104 1357 1403 2611 104
Size: 1253 46 1208 1308
Fragments arranged by size:
1308 1253 1208 46
BsiHKAI G_wGCw'C
Cuts at: 338 829 1607 1797 2589 338
Size: 491 778 190 792 1564
Fragments arranged by size:
1564 792 778 491 190
BsiWI C'GTAC_G
Cuts at: 122 122
Size: 3815
Bsp1286I G_dGCh'C
Cuts at: 338 713 785 829 1440 1533 1607 1797 1859
Size: 375 72 44 611 93 74 190 62
Cuts at: 1859 2589 338
Size: 730 1564
Fragments arranged by size:
1564 730 611 375 190 93 74 72
62 44
BspEI T'CCGG_A
Cuts at: 3035 3035
Size: 3815
BspHI T'CATG_A
Cuts at: 2991 2991
Size: 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 358 489 865 1079 1381 1762 358
Size: 131 376 214 302 381 2411
Fragments arranged by size:
2411 381 376 302 214 131
BsrBI CCG'CTC
Cuts at: 50 130 2107 2161 3645 3810 50
Size: 80 1977 54 1484 165 55
Fragments arranged by size:
1977 1484 165 80 55 54
BsrDI GCAATG_nn'
Cuts at: 1727 1727
Size: 3815
BsrFI r'CCGG_y
Cuts at: 733 759 1813 1994 2203 733
Size: 26 1054 181 209 2345
Fragments arranged by size:
2345 1054 209 181 26
BssSI C'ACGA_G
Cuts at: 2086 2444 2086
Size: 358 3457
BstAPI GCAn_nnn'nTGC
Cuts at: 1192 1192
Size: 3815
BstBI TT'CG_AA
Cuts at: 2176 2176
Size: 3815
BstYI r'GATC_y
Cuts at: 1 726 840 1307 1330 1665 1911 2923 3009
Size: 725 114 467 23 335 246 1012 86
Cuts at: 3009 3021 3367 1
Size: 12 346 449
Fragments arranged by size:
1012 725 467 449 346 335 246 114
86 23 12
BtgI C'CryG_G
Cuts at: 107 326 107
Size: 219 3596
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 543 1765 1946 2109 543
Size: 1222 181 163 2249
Fragments arranged by size:
2249 1222 181 163
BtsI GCAGTG_nn'
Cuts at: 1058 1058
Size: 3815
DraI TTT'AAA
Cuts at: 814 814
Size: 3815
DrdI GACnn_nn'nnGTC
Cuts at: 436 1521 2379 436
Size: 1085 858 1872
Fragments arranged by size:
1872 1085 858
EaeI y'GGCC_r
Cuts at: 323 485 761 1400 1574 1965 1992 3337 323
Size: 162 276 639 174 391 27 1345 801
Fragments arranged by size:
1345 801 639 391 276 174 162 27
EagI C'GGCC_G
Cuts at: 1400 1400
Size: 3815
EarI CTCTTCn'nnn_
Cuts at: 652 1838 2048 652
Size: 1186 210 2419
Fragments arranged by size:
2419 1186 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2023 2333 2479 3625 2023
Size: 310 146 1146 2213
Fragments arranged by size:
2213 1146 310 146
EcoICRI GAG'CTC
Cuts at: 827 827
Size: 3815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 666 1640 2072 2819 3087 666
Size: 974 432 747 268 1394
Fragments arranged by size:
1394 974 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 954 954
Size: 3815
EcoRI G'AATT_C
Cuts at: 819 819
Size: 3815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3815
FspI TGC'GCA
Cuts at: 1596 1596
Size: 3815
HaeII r_GCGC'y
Cuts at: 149 263 635 1249 1497 2519 3498 149
Size: 114 372 614 248 1022 979 466
Fragments arranged by size:
1022 979 614 466 372 248 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1657 1687 1689 1719 1657
Size: 30 2 30 3753
Fragments arranged by size:
3753 30 30 2
HincII GTy'rAC
Cuts at: 854 854
Size: 3815
KasI G'GCGC_C
Cuts at: 145 631 1245 1493 145
Size: 486 614 248 2467
Fragments arranged by size:
2467 614 486 248
KpnI G_GTAC'C
Cuts at: 624 835 624
Size: 211 3604
MluI A'CGCG_T
Cuts at: 3248 3248
Size: 3815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 452 2486 2670 452
Size: 2034 184 1597
Fragments arranged by size:
2034 1597 184
MscI TGG'CCA
Cuts at: 487 1576 487
Size: 1089 2726
MslI CAynn'nnrTG
Cuts at: 220 1804 1931 220
Size: 1584 127 2104
Fragments arranged by size:
2104 1584 127
MspA1I CmG'CkG
Cuts at: 618 926 1240 1600 2613 2858 618
Size: 308 314 360 1013 245 1575
Fragments arranged by size:
1575 1013 360 314 308 245
NaeI GCC'GGC
Cuts at: 761 1996 761
Size: 1235 2580
NarI GG'CG_CC
Cuts at: 146 632 1246 1494 146
Size: 486 614 248 2467
Fragments arranged by size:
2467 614 486 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3815
NgoMIV G'CCGG_C
Cuts at: 759 1994 759
Size: 1235 2580
NspI r_CATG'y
Cuts at: 690 868 1368 2275 690
Size: 178 500 907 2230
Fragments arranged by size:
2230 907 500 178
PasI CC'CwG_GG
Cuts at: 786 786
Size: 3815
PciI A'CATG_T
Cuts at: 2271 2271
Size: 3815
PflMI CCAn_nnn'nTGG
Cuts at: 494 494
Size: 3815
PmeI GTTT'AAAC
Cuts at: 814 814
Size: 3815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 3783
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 232 264 232
Size: 32 3783
PstI C_TGCA'G
Cuts at: 313 862 1547 313
Size: 549 685 2581
Fragments arranged by size:
2581 685 549
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3815
PvuII CAG'CTG
Cuts at: 1240 1600 1240
Size: 360 3455
RsrII CG'GwC_CG
Cuts at: 2010 2010
Size: 3815
SacI G_AGCT'C
Cuts at: 829 829
Size: 3815
SalI G'TCGA_C
Cuts at: 852 852
Size: 3815
SapI GCTCTTCn'nnn_
Cuts at: 1838 2048 1838
Size: 210 3605
SbfI CC_TGCA'GG
Cuts at: 862 862
Size: 3815
ScaI AGT'ACT
Cuts at: 3332 3332
Size: 3815
SfcI C'TryA_G
Cuts at: 33 309 858 1543 2536 2727 33
Size: 276 549 685 993 191 1121
Fragments arranged by size:
1121 993 685 549 276 191
SfoI GGC'GCC
Cuts at: 147 633 1247 1495 147
Size: 486 614 248 2467
Fragments arranged by size:
2467 614 486 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
SmaI CCC'GGG
Cuts at: 550 837 550
Size: 287 3528
SmlI C'TyrA_G
Cuts at: 970 1885 2377 2639 970
Size: 915 492 262 2146
Fragments arranged by size:
2146 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3815
SphI G_CATG'C
Cuts at: 690 868 1368 690
Size: 178 500 3137
Fragments arranged by size:
3137 500 178
SrfI GCCC'GGGC
Cuts at: 550 550
Size: 3815
StyI C'CwwG_G
Cuts at: 107 512 949 1303 107
Size: 405 437 354 2619
Fragments arranged by size:
2619 437 405 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1398 1531 1990 2199 1398
Size: 133 459 209 3014
Fragments arranged by size:
3014 459 209 133
TatI w'GTAC_w
Cuts at: 377 3330 377
Size: 2953 862
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 3783
Tth111I GACn'n_nGTC
Cuts at: 1612 1612
Size: 3815
XbaI T'CTAG_A
Cuts at: 846 846
Size: 3815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1310 1310
Size: 3815
XmaI C'CCGG_G
Cuts at: 548 835 548
Size: 287 3528
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
Bpu10I BsgI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI ZraI