Restriction map for pF1KB7778
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp718I     AspI        
AviII       AvrII       BamHI       BbrPI       BbvCI       
BcgI        BciVI       BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrGI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstPI       Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        MfeI        MluI        MroI        
MseI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tsp509I     Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        1185  GACGTC               GACGT^C
AccI          582  GTATAC               GT^[AC][GT]AC
AhdI          244  GACTCGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1084  GTGCAC               G^TGCAC
AlwI         1401  GATCC                GGATC(4/5)
ApaLI        1084  GTGCAC               G^TGCAC
Asp700I      1293  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         244  GACTCGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1084  GTGCAC               G[AT]GC[AT]^C
BclI          614  TGATCA               T^GATCA
BglI           22  GCCGCGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         732  AGATCT               A^GATCT
BsaBI         312  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI          68  ACCGGA               [AT]^CCGG[AT]
BsiHKAI      1084  GTGCAC               G[AT]GC[AT]^C
BsmBI        1106  CGTCTC               CGTCTC(1/5)
BsmI         1293  GAATGC               GAATGC(1/-1)
Bst1107I      582  GTATAC               GTA^TAC
BstZ17I       582  GTATAC               GTA^TAC
DdeI          304  CTGAG                C^T[ACGT]AG
Eam1105I      244  GACTCGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I       776  CCAAGG               C^C[AT][AT]GG
FbaI          614  TGATCA               T^GATCA
MaeII        1186  ACGT                 A^CGT
MamI          312  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI          792  GCCGGC               GCC^GGC
NgoMIV        792  GCCGGC               G^CCGGC
NspI         1236  GCATGC               [GA]CATG^[CT]
PflMI        1425  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          604  CTGCAG               CTGCA^G
PvuII        1012  CAGCTG               CAG^CTG
SexAI         743  ACCAGGT              A^CC[AT]GGT
SphI         1236  GCATGC               GCATG^C
StuI          512  AGGCCT               AGG^CCT
StyI          776  CCAAGG               C^C[AT][AT]GG
TaqI          452  TCGA                 T^CGA
TfiI          642  GATTC                G^A[AT]TC
TthHB8I       452  TCGA                 T^CGA
Van91I       1425  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1293  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AlwNI         544  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              914  CAGTGTCTG           
BalI          560  TGGCCA               TGG^CCA
             1212  TGGCCA              
BbeI          204  GGCGCC               GGCGC^C
              274  GGCGCC              
BbsI          414  GAAGAC               GAAGAC(2/6)
              492  GAAGAC              
BpmI         1149  CTCCAG               CTGGAG(16/14)
              166  CTGGAG              
BpuAI         414  GAAGAC               GAAGAC(2/6)
              492  GAAGAC              
BsaI          625  GAGACC               GGTCTC(1/5)
              772  GAGACC              
BsgI         1069  GTGCAG               GTGCAG(16/14)
             1396  GTGCAG              
BsiEI          85  CGGCCG               CG[GA][CT]^CG
              159  CGGCCG              
BspHI         503  TCATGA               T^CATGA
             1181  TCATGA              
BstXI         899  CCAACTACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1199  CCACAACTGTGG        
BstYI         732  AGATCT               [GA]^GATC[CT]
             1400  AGATCC              
DsaI           23  CCGCGG               C^C[GA][CT]GG
              212  CCGCGG              
EagI           85  CGGCCG               C^GGCCG
              159  CGGCCG              
EarI          724  GAAGAG               CTCTTC(1/4)
              969  GAAGAG              
EclXI          85  CGGCCG               C^GGCCG
              159  CGGCCG              
Eco52I         85  CGGCCG               C^GGCCG
              159  CGGCCG              
KasI          204  GGCGCC               G^GCGCC
              274  GGCGCC              
Ksp632I       724  GAAGAG               CTCTTC(1/4)
              969  GAAGAG              
KspI           23  CCGCGG               CCGC^GG
              212  CCGCGG              
MflI          732  AGATCT               [GA]^GATC[CT]
             1400  AGATCC              
MluNI         560  TGGCCA               TGG^CCA
             1212  TGGCCA              
MscI          560  TGGCCA               TGG^CCA
             1212  TGGCCA              
NarI          204  GGCGCC               GG^CGCC
              274  GGCGCC              
PpuMI         128  GGGTCCC              [GA]G^G[AT]CC[CT]
             1061  AGGTCCC             
RcaI          503  TCATGA               T^CATGA
             1181  TCATGA              
SacII          23  CCGCGG               CCGC^GG
              212  CCGCGG              
SfaNI         500  GCATC                GCATC(5/9)
             1381  GCATC               
SfoI          204  GGCGCC               GGC^GCC
              274  GGCGCC              
SmaI          133  CCCGGG               CCC^GGG
              419  CCCGGG              
XcmI         1256  CCACCTCGGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1344  CCAGGAGCCAGGTGG     
XhoII         732  AGATCT               [GA]^GATC[CT]
             1400  AGATCC              
XmaCI         133  CCCGGG               C^CCGGG
              419  CCCGGG              
XmaI          133  CCCGGG               C^CCGGG
              419  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
ApaI        AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BfaI        BmyI        BsaHI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DpnII       DraII       EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfcI        
TseI        Tsp45I      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com