Restriction map for pF1KB7778
 (Linear) (Six-base) MAPSORT of: KIBB7778.seq  Check: 9334  from: 1  to: 1524



KIBB7778.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1314    1524 
   Size:       1314     210
 
AatII G_ACGT'C

Cuts at:      0    1189    1524 
   Size:       1189     335
 
AccI GT'mk_AC

Cuts at:      0     583    1524 
   Size:        583     941
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     465     808     988    1524 
   Size:        313     152     343     180     536

  Fragments arranged by size: 

                 536     343     313     180     152
 
AhdI GACnn_n'nnGTC

Cuts at:      0     249    1524 
   Size:        249    1275
 
AleI CACnn'nnGTG

Cuts at:      0    1204    1524 
   Size:       1204     320
 
AlwNI CAG_nnn'CTG

Cuts at:      0     549     919    1524 
   Size:        549     370     605

  Fragments arranged by size: 

                 605     549     370
 
ApaI G_GGCC'C

Cuts at:      0     158     222     341    1524 
   Size:        158      64     119    1183

  Fragments arranged by size: 

                1183     158     119      64
 
ApaLI G'TGCA_C

Cuts at:      0    1084    1524 
   Size:       1084     440
 
AvaI C'yCGr_G

Cuts at:      0     133     151     340     419    1260    1524 
   Size:        133      18     189      79     841     264

  Fragments arranged by size: 

                 841     264     189     133      79      18
 
BanI G'GyrC_C

Cuts at:      0     204     274     909    1308    1524 
   Size:        204      70     635     399     216

  Fragments arranged by size: 

                 635     399     216     204      70
 
BanII G_rGCy'C

Cuts at:      0      46     152     158     222     341    1524 
   Size:         46     106       6      64     119    1183

  Fragments arranged by size: 

                1183     119     106      64      46       6
 
BbeI G_GCGC'C

Cuts at:      0     208     278    1524 
   Size:        208      70    1246

  Fragments arranged by size: 

                1246     208      70
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     421     499    1524 
   Size:        421      78    1025

  Fragments arranged by size: 

                1025     421      78
 
BclI T'GATC_A

Cuts at:      0     614    1524 
   Size:        614     910
 
BglI GCCn_nnn'nGGC

Cuts at:      0      28    1524 
   Size:         28    1496
 
BglII A'GATC_T

Cuts at:      0     732    1524 
   Size:        732     792
 
Bme1580I G_kGCm'C

Cuts at:      0     158     222     341    1088    1199    1524 
   Size:        158      64     119     747     111     325

  Fragments arranged by size: 

                 747     325     158     119     111      64
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     929    1060    1524 
   Size:        929     131     464

  Fragments arranged by size: 

                 929     464     131
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     261    1524 
   Size:        261    1263
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187    1134    1524 
   Size:        187     947     390

  Fragments arranged by size: 

                 947     390     187
 
Bpu10I CC'TnA_GC

Cuts at:      0     304    1524 
   Size:        304    1220
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     619     766    1524 
   Size:        619     147     758

  Fragments arranged by size: 

                 758     619     147
 
BsaBI GATnn'nnATC

Cuts at:      0     316    1524 
   Size:        316    1208
 
BsaHI Gr'CG_yC

Cuts at:      0     205     275     346    1186    1358    1524 
   Size:        205      70      71     840     172     166

  Fragments arranged by size: 

                 840     205     172     166      71      70
 
BsaWI w'CCGG_w

Cuts at:      0      68    1524 
   Size:         68    1456
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     274     277     280     984    1137    1524 
   Size:        274       3       3     704     153     387

  Fragments arranged by size: 

                 704     387     274     153       3       3
 
BseYI C'CCAG_C

Cuts at:      0      16     270    1055    1524 
   Size:         16     254     785     469

  Fragments arranged by size: 

                 785     469     254      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1090    1417    1524 
   Size:       1090     327     107

  Fragments arranged by size: 

                1090     327     107
 
BsiEI CG_ry'CG

Cuts at:      0      88     162    1524 
   Size:         88      74    1362

  Fragments arranged by size: 

                1362      88      74
 
BsiHKAI G_wGCw'C

Cuts at:      0    1088    1524 
   Size:       1088     436
 
BsmI GAATG_Cn'

Cuts at:      0    1299    1524 
   Size:       1299     225
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1112    1524 
   Size:       1112     412
 
Bsp1286I G_dGCh'C

Cuts at:      0      46     152     158     222     341    1088    1199    1524 
   Size:         46     106       6      64     119     747     111     325

  Fragments arranged by size: 

                 747     325     119     111     106      64      46       6
            
 
BspHI T'CATG_A

Cuts at:      0     503    1181    1524 
   Size:        503     678     343

  Fragments arranged by size: 

                 678     503     343
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     658     821    1050    1314    1524 
   Size:        658     163     229     264     210

  Fragments arranged by size: 

                 658     264     229     210     163
 
BsrBI CCG'CTC

Cuts at:      0     171     282     383     395    1524 
   Size:        171     111     101      12    1129

  Fragments arranged by size: 

                1129     171     111     101      12
 
BsrFI r'CCGG_y

Cuts at:      0     375     628     792    1524 
   Size:        375     253     164     732

  Fragments arranged by size: 

                 732     375     253     164
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     906    1206    1524 
   Size:        906     300     318

  Fragments arranged by size: 

                 906     318     300
 
BstYI r'GATC_y

Cuts at:      0     732    1400    1524 
   Size:        732     668     124

  Fragments arranged by size: 

                 732     668     124
 
BstZ17I GTA'TAC

Cuts at:      0     584    1524 
   Size:        584     940
 
BtgI C'CryG_G

Cuts at:      0      23     212    1524 
   Size:         23     189    1312

  Fragments arranged by size: 

                1312     189      23
 
BtsI GCAGTG_nn'

Cuts at:      0     771     954    1055    1524 
   Size:        771     183     101     469

  Fragments arranged by size: 

                 771     469     183     101
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     702     737    1524 
   Size:        702      35     787

  Fragments arranged by size: 

                 787     702      35
 
EaeI y'GGCC_r

Cuts at:      0      85     159     356     560    1212    1524 
   Size:         85      74     197     204     652     312

  Fragments arranged by size: 

                 652     312     204     197      85      74
 
EagI C'GGCC_G

Cuts at:      0      85     159    1524 
   Size:         85      74    1365

  Fragments arranged by size: 

                1365      85      74
 
EarI CTCTTCn'nnn_

Cuts at:      0     719     964    1524 
   Size:        719     245     560

  Fragments arranged by size: 

                 719     560     245
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     313     465     808     988    1134    1524 
   Size:        187     126     152     343     180     146     390

  Fragments arranged by size: 

                 390     343     187     180     152     146     126
 
EcoO109I rG'GnC_Cy

Cuts at:      0     129     218     219    1002    1062    1092    1524 
   Size:        129      89       1     783      60      30     432

  Fragments arranged by size: 

                 783     432     129      89      60      30       1
 
HaeII r_GCGC'y

Cuts at:      0     208     278     471    1524 
   Size:        208      70     193    1053

  Fragments arranged by size: 

                1053     208     193      70
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     235     267    1524 
   Size:        235      32    1257

  Fragments arranged by size: 

                1257     235      32
 
KasI G'GCGC_C

Cuts at:      0     204     274    1524 
   Size:        204      70    1250

  Fragments arranged by size: 

                1250     204      70
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     731    1524 
   Size:        731     793
 
MscI TGG'CCA

Cuts at:      0     562    1214    1524 
   Size:        562     652     310

  Fragments arranged by size: 

                 652     562     310
 
MslI CAynn'nnrTG

Cuts at:      0    1033    1081    1180    1204    1324    1524 
   Size:       1033      48      99      24     120     200

  Fragments arranged by size: 

                1033     200     120      99      48      24
 
MspA1I CmG'CkG

Cuts at:      0      25     187     214     230     355     878     983    1014 
   Size:         25     162      27      16     125     523     105      31

Cuts at:   1014    1154    1524 
   Size:        140     370

  Fragments arranged by size: 

                 523     370     162     140     125     105      31      27
                  25      16
 
NaeI GCC'GGC

Cuts at:      0     794    1524 
   Size:        794     730
 
NarI GG'CG_CC

Cuts at:      0     205     275    1524 
   Size:        205      70    1249

  Fragments arranged by size: 

                1249     205      70
 
NgoMIV G'CCGG_C

Cuts at:      0     792    1524 
   Size:        792     732
 
NspI r_CATG'y

Cuts at:      0    1240    1524 
   Size:       1240     284
 
PasI CC'CwG_GG

Cuts at:      0      51    1524 
   Size:         51    1473
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1431    1524 
   Size:       1431      93
 
PfoI T'CCnGG_A

Cuts at:      0    1343    1524 
   Size:       1343     181
 
PpuMI rG'GwC_Cy

Cuts at:      0     129    1062    1524 
   Size:        129     933     462

  Fragments arranged by size: 

                 933     462     129
 
PspOMI G'GGCC_C

Cuts at:      0     154     218     337    1524 
   Size:        154      64     119    1187

  Fragments arranged by size: 

                1187     154     119      64
 
PstI C_TGCA'G

Cuts at:      0     608    1524 
   Size:        608     916
 
PvuII CAG'CTG

Cuts at:      0    1014    1524 
   Size:       1014     510
 
SacII CC_GC'GG

Cuts at:      0      26     215    1524 
   Size:         26     189    1309

  Fragments arranged by size: 

                1309     189      26
 
SanDI GG'GwC_CC

Cuts at:      0     129    1524 
   Size:        129    1395
 
SexAI A'CCwGG_T

Cuts at:      0     743    1524 
   Size:        743     781
 
SfcI C'TryA_G

Cuts at:      0     225     604    1480    1524 
   Size:        225     379     876      44

  Fragments arranged by size: 

                 876     379     225      44
 
SfoI GGC'GCC

Cuts at:      0     206     276    1524 
   Size:        206      70    1248

  Fragments arranged by size: 

                1248     206      70
 
SmaI CCC'GGG

Cuts at:      0     135     421    1524 
   Size:        135     286    1103

  Fragments arranged by size: 

                1103     286     135
 
SphI G_CATG'C

Cuts at:      0    1240    1524 
   Size:       1240     284
 
StuI AGG'CCT

Cuts at:      0     514    1524 
   Size:        514    1010
 
StyI C'CwwG_G

Cuts at:      0     776    1524 
   Size:        776     748
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1198    1258    1524 
   Size:       1198      60     266

  Fragments arranged by size: 

                1198     266      60
 
TatI w'GTAC_w

Cuts at:      0     973    1524 
   Size:        973     551
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1263    1351    1524 
   Size:       1263      88     173

  Fragments arranged by size: 

                1263     173      88
 
XmaI C'CCGG_G

Cuts at:      0     133     419    1524 
   Size:        133     286    1105

  Fragments arranged by size: 

                1105     286     133
 
XmnI GAAnn'nnTTC

Cuts at:      0    1297    1524 
   Size:       1297     227
 
ZraI GAC'GTC

Cuts at:      0    1187    1524 
   Size:       1187     337
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AhdI     AleI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII     BbeI     BbsI     BclI     BglI
    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BstXI    BstYI
  BstZ17I     BtgI     BtsI    CspCI     EaeI     EagI     EarI  Eco57MI
 EcoO109I    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI
   PspOMI     PstI    PvuII    SacII    SanDI    SexAI     SfcI     SfoI
     SmaI     SphI     StuI     StyI    TaqII     TatI     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AlfI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
     BcgI    BciVI    BfrBI     BlpI    BmgBI     BmtI    BpuEI    BsaAI
    BsaXI    BsiWI    BspEI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XhoI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com