Restriction map for pF1KB9552
 (Linear) (Six-base) MAPSORT of: KIBB9552.seq  Check: 7516  from: 1  to: 1398



KIBB9552.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     392    1398 
   Size:        392    1006
 
AlwNI CAG_nnn'CTG

Cuts at:      0     900    1398 
   Size:        900     498
 
ApaI G_GGCC'C

Cuts at:      0      98    1088    1097    1289    1340    1398 
   Size:         98     990       9     192      51      58

  Fragments arranged by size: 

                 990     192      98      58      51       9
 
AvaI C'yCGr_G

Cuts at:      0      72     135     682    1242    1398 
   Size:         72      63     547     560     156

  Fragments arranged by size: 

                 560     547     156      72      63
 
BanI G'GyrC_C

Cuts at:      0     261     286     644     914    1398 
   Size:        261      25     358     270     484

  Fragments arranged by size: 

                 484     358     270     261      25
 
BanII G_rGCy'C

Cuts at:      0      40      49      59      98     345     401     661    1088 
   Size:         40       9      10      39     247      56     260     427

Cuts at:   1088    1097    1289    1340    1398 
   Size:          9     192      51      58

  Fragments arranged by size: 

                 427     260     247     192      58      56      51      40
                  39      10       9       9
 
BbeI G_GCGC'C

Cuts at:      0     290    1398 
   Size:        290    1108
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     742    1075    1398 
   Size:        742     333     323

  Fragments arranged by size: 

                 742     333     323
 
BbvCI CC'TCA_GC

Cuts at:      0    1147    1398 
   Size:       1147     251
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     345     379    1398 
   Size:        345      34    1019

  Fragments arranged by size: 

                1019     345      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      44     528     610     912    1092    1244    1398 
   Size:         44     484      82     302     180     152     154

  Fragments arranged by size: 

                 484     302     180     154     152      82      44
 
BlpI GC'TnA_GC

Cuts at:      0      34     238     395     400     470    1167    1398 
   Size:         34     204     157       5      70     697     231

  Fragments arranged by size: 

                 697     231     204     157      70      34       5
 
Bme1580I G_kGCm'C

Cuts at:      0      98     266     571     917     969    1088    1097    1289 
   Size:         98     168     305     346      52     119       9     192

Cuts at:   1289    1340    1398 
   Size:         51      58

  Fragments arranged by size: 

                 346     305     192     168     119      98      58      52
                  51       9
 
BmgBI CAC'GTC

Cuts at:      0     309    1398 
   Size:        309    1089
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1188    1335    1371    1398 
   Size:       1188     147      36      27

  Fragments arranged by size: 

                1188     147      36      27
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     708    1398 
   Size:        708     690
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444    1398 
   Size:        444     954
 
Bpu10I CC'TnA_GC

Cuts at:      0     344    1147    1398 
   Size:        344     803     251

  Fragments arranged by size: 

                 803     344     251
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     862    1038    1069    1398 
   Size:        862     176      31     329

  Fragments arranged by size: 

                 862     329     176      31
 
BsaHI Gr'CG_yC

Cuts at:      0     287    1398 
   Size:        287    1111
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     766     796    1398 
   Size:        766      30     602

  Fragments arranged by size: 

                 766     602      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22      67     478    1398 
   Size:         22      45     411     920

  Fragments arranged by size: 

                 920     411      45      22
 
BseYI C'CCAG_C

Cuts at:      0     150     411     957    1324    1398 
   Size:        150     261     546     367      74

  Fragments arranged by size: 

                 546     367     261     150      74
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370    1398 
   Size:        370    1028
 
BsiHKAI G_wGCw'C

Cuts at:      0     239     401     689    1398 
   Size:        239     162     288     709

  Fragments arranged by size: 

                 709     288     239     162
 
Bsp1286I G_dGCh'C

Cuts at:      0      40      49      59      98     239     266     345     401 
   Size:         40       9      10      39     141      27      79      56

Cuts at:    401     571     661     689     917     969    1088    1097    1289 
   Size:        170      90      28     228      52     119       9     192

Cuts at:   1289    1340    1398 
   Size:         51      58

  Fragments arranged by size: 

                 228     192     170     141     119      90      79      58
                  56      52      51      40      39      28      27      10
                   9       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     392     908    1398 
   Size:        392     516     490

  Fragments arranged by size: 

                 516     490     392
 
BsrBI CCG'CTC

Cuts at:      0     474    1398 
   Size:        474     924
 
BsrFI r'CCGG_y

Cuts at:      0     641     946    1398 
   Size:        641     305     452

  Fragments arranged by size: 

                 641     452     305
 
BssHII G'CGCG_C

Cuts at:      0      80     352    1398 
   Size:         80     272    1046

  Fragments arranged by size: 

                1046     272      80
 
BssSI C'ACGA_G

Cuts at:      0     847    1398 
   Size:        847     551
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     706    1255    1398 
   Size:        706     549     143

  Fragments arranged by size: 

                 706     549     143
 
BstYI r'GATC_y

Cuts at:      0      15     998    1398 
   Size:         15     983     400

  Fragments arranged by size: 

                 983     400      15
 
Bsu36I CC'TnA_GG

Cuts at:      0     693    1221    1332    1398 
   Size:        693     528     111      66

  Fragments arranged by size: 

                 693     528     111      66
 
BtgI C'CryG_G

Cuts at:      0      41     705     785     974    1028    1079    1398 
   Size:         41     664      80     189      54      51     319

  Fragments arranged by size: 

                 664     319     189      80      54      51      41
 
BtsI GCAGTG_nn'

Cuts at:      0     969    1398 
   Size:        969     429
 
DraIII CAC_nnn'GTG

Cuts at:      0    1045    1398 
   Size:       1045     353
 
EaeI y'GGCC_r

Cuts at:      0     971     977    1236    1310    1398 
   Size:        971       6     259      74      88

  Fragments arranged by size: 

                 971     259      88      74       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     128     202     546     577     981    1398 
   Size:         19     109      74     344      31     404     417

  Fragments arranged by size: 

                 417     404     344     109      74      31      19
 
EcoICRI GAG'CTC

Cuts at:      0     399    1398 
   Size:        399     999
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444    1398 
   Size:        444     954
 
EcoO109I rG'GnC_Cy

Cuts at:      0      94    1084    1085    1093    1094    1225    1285    1286 
   Size:         94     990       1       8       1     131      60       1

Cuts at:   1286    1336    1337    1398 
   Size:         50       1      61

  Fragments arranged by size: 

                 990     131      94      61      60      50       8       1
                   1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     471    1398 
   Size:        471     927
 
HaeII r_GCGC'y

Cuts at:      0     290     453     629     783    1398 
   Size:        290     163     176     154     615

  Fragments arranged by size: 

                 615     290     176     163     154
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     733     765     766     798     841     873    1102    1134 
   Size:        733      32       1      32      43      32     229      32

Cuts at:   1134    1398 
   Size:        264

  Fragments arranged by size: 

                 733     264     229      43      32      32      32      32
                   1
 
HincII GTy'rAC

Cuts at:      0     282    1398 
   Size:        282    1116
 
KasI G'GCGC_C

Cuts at:      0     286    1398 
   Size:        286    1112
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     748     869     884    1398 
   Size:        748     121      15     514

  Fragments arranged by size: 

                 748     514     121      15
 
MscI TGG'CCA

Cuts at:      0     979    1238    1398 
   Size:        979     259     160

  Fragments arranged by size: 

                 979     259     160
 
MslI CAynn'nnrTG

Cuts at:      0     271    1398 
   Size:        271    1127
 
MspA1I CmG'CkG

Cuts at:      0      62     242    1081    1296    1398 
   Size:         62     180     839     215     102

  Fragments arranged by size: 

                 839     215     180     102      62
 
NaeI GCC'GGC

Cuts at:      0     948    1398 
   Size:        948     450
 
NarI GG'CG_CC

Cuts at:      0     287    1398 
   Size:        287    1111
 
NcoI C'CATG_G

Cuts at:      0     705     785    1398 
   Size:        705      80     613

  Fragments arranged by size: 

                 705     613      80
 
NgoMIV G'CCGG_C

Cuts at:      0     946    1398 
   Size:        946     452
 
PasI CC'CwG_GG

Cuts at:      0     552    1089    1398 
   Size:        552     537     309

  Fragments arranged by size: 

                 552     537     309
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1361    1398 
   Size:       1361      37
 
PpuMI rG'GwC_Cy

Cuts at:      0    1225    1398 
   Size:       1225     173
 
PspOMI G'GGCC_C

Cuts at:      0      94    1084    1093    1285    1336    1398 
   Size:         94     990       9     192      51      62

  Fragments arranged by size: 

                 990     192      94      62      51       9
 
PstI C_TGCA'G

Cuts at:      0     749    1398 
   Size:        749     649
 
SacI G_AGCT'C

Cuts at:      0     401    1398 
   Size:        401     997
 
SacII CC_GC'GG

Cuts at:      0    1082    1398 
   Size:       1082     316
 
SexAI A'CCwGG_T

Cuts at:      0    1041    1398 
   Size:       1041     357
 
SfcI C'TryA_G

Cuts at:      0     745    1398 
   Size:        745     653
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1092    1244    1398 
   Size:       1092     152     154

  Fragments arranged by size: 

                1092     154     152
 
SfoI GGC'GCC

Cuts at:      0     288    1398 
   Size:        288    1110
 
SmaI CCC'GGG

Cuts at:      0      74     137    1398 
   Size:         74      63    1261

  Fragments arranged by size: 

                1261      74      63
 
StuI AGG'CCT

Cuts at:      0    1252    1398 
   Size:       1252     146
 
StyI C'CwwG_G

Cuts at:      0     705     785    1398 
   Size:        705      80     613

  Fragments arranged by size: 

                 705     613      80
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     662     729     932    1398 
   Size:        662      67     203     466

  Fragments arranged by size: 

                 662     466     203      67
 
Tth111I GACn'n_nGTC

Cuts at:      0    1386    1398 
   Size:       1386      12
 
XmaI C'CCGG_G

Cuts at:      0      72     135    1398 
   Size:         72      63    1263

  Fragments arranged by size: 

                1263      72      63
 
XmnI GAAnn'nnTTC

Cuts at:      0     328    1398 
   Size:        328    1070
 

 Enzymes that do cut:

     AarI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstXI
    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EciI  EcoICRI
  Eco57MI EcoO109I     FalI    HaeII    Hin4I   HincII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI
    PflMI    PpuMI   PspOMI     PstI     SacI    SacII    SexAI     SfcI
     SfiI     SfoI     SmaI     StuI     StyI    TaqII  Tth111I     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI     BclI    BfrBI
    BglII     BmtI    BpuEI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BstAPI    BstBI
   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EarI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SalI
    SanDI     SapI     SbfI     ScaI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI     XbaI
     XcmI     XhoI     ZraI
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