Restriction map for pF1KB6320
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AviII       AvrII       BamHI       BbrPI       
BbsI        BbvCI       BcgI        BclI        BfaI        
BfrI        BglI        BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HpaI        KspI        MaeI        MaeII       MaeIII      
MamI        MfeI        MluI        MroI        MspA1I      
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SapI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SspBI       SspI        StyI        SwaI        TaqI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        717  GGTACC               G^GTACC
AccII         781  CGCG                 CG^CG
AcyI          447  GGCGCC               G[GA]^CG[CT]C
AlwNI         115  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       717  GGTACC               G^GTACC
AspHI         661  GAGCTC               G[AT]GC[AT]^C
BanII         661  GAGCTC               G[GA]GC[CT]^C
BbeI          447  GGCGCC               GGCGC^C
BciVI         248  GTATCC               GTATCC(6/5)
BglII         827  AGATCT               A^GATCT
BmyI          661  GAGCTC               G[AGT]GC[ACT]^C
BsaHI         447  GGCGCC               G[GA]^CG[CT]C
BseRI         649  GAGGAG               GAGGAG(10/8)
BsiHKAI       661  GAGCTC               G[AT]GC[AT]^C
Bsp1286I      661  GAGCTC               G[AGT]GC[ACT]^C
BspMI         641  ACCTGC               ACCTGC(4/8)
BsrBI         307  GAGCGG               CCGCTC(-3/-3)
BsrDI         279  CATTGC               GCAATG(2/0)
BstF5I         69  GGATG                GGATG(2/0)
BstUI         781  CGCG                 CG^CG
BstXI         389  CCACCCCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII         414  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI          395  CCGTGG               C^C[GA][CT]GG
EarI          327  GAAGAG               CTCTTC(1/4)
EcoNI         642  CCTGCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      414  GGGGCCT              [GA]G^G[ACGT]CC[CT]
FokI           69  GGATG                GGATG(9/13)
Hin1I         447  GGCGCC               G[GA]^CG[CT]C
HphI          635  TCACC                GGTGA(8/7)
KasI          447  GGCGCC               G^GCGCC
KpnI          717  GGTACC               GGTAC^C
Ksp632I       327  GAAGAG               CTCTTC(1/4)
MseI          800  TTAA                 T^TAA
MvnI          781  CGCG                 CG^CG
NarI          447  GGCGCC               GG^CGCC
NruI          780  TCGCGA               TCG^CGA
PflMI         114  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          790  CTGCAG               CTGCA^G
SacI          661  GAGCTC               GAGCT^C
SbfI          789  CCTGCAGG             CCTGCA^GG
SfcI          790  CTGCAG               C^T[GA][CT]AG
SfoI          447  GGCGCC               GGC^GCC
SphI          428  GCATGC               GCATG^C
Sse8387I      789  CCTGCAGG             CCTGCA^GG
Tru9I         800  TTAA                 T^TAA
Van91I        114  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          389  CCACCCCCGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AvaII         312  GGACC                G^G[AT]CC
              708  GGTCC               
BalI          111  TGGCCA               TGG^CCA
              531  TGGCCA              
BsrI          105  CCAGT                ACTGG(1/-1)
              252  CCAGT               
BstAPI        462  GCACCACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              564  GCAGCTTATGC         
EcoRV         124  GATATC               GAT^ATC
              274  GATATC              
HaeII         190  AGCGCC               [GA]GCGC^[CT]
              447  GGCGCC              
MluNI         111  TGGCCA               TGG^CCA
              531  TGGCCA              
MscI          111  TGGCCA               TGG^CCA
              531  TGGCCA              
MslI          297  CACCTTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              390  CACCCCCGTG          
PleI          268  GACTC                GAGTC(4/5)
              441  GACTC               
SfaNI         582  GATGC                GCATC(5/9)
              784  GATGC               
SmaI          423  CCCGGG               CCC^GGG
              721  CCCGGG              
StuI          161  AGGCCT               AGG^CCT
              519  AGGCCT              
TfiI          609  GAATC                G^A[AT]TC
              618  GAATC               
XmaCI         423  CCCGGG               C^CCGGG
              721  CCCGGG              
XmaI          423  CCCGGG               C^CCGGG
              721  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaI        
BanI        BbvI        BcnI        BsaI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
BssKI       BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
FauI        Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MboI        
MboII       MflI        MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        RsaI        Sau3AI      Sau96I      ScrFI       
TseI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com