Restriction map for
pF1KB7664
(Circular) (Six-base) MAPSORT of: pF1KB7664.seq Check: 678 from: 1 to: 4301
pF1KB7664 4301 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 960 1339 960
Size: 379 3922
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 2558 3305 3573 2126
Size: 432 747 268 2854
Fragments arranged by size:
2854 747 432 268
AflIII A'CryG_T
Cuts at: 1653 2757 3734 1653
Size: 1104 977 2220
Fragments arranged by size:
2220 1104 977
AgeI A'CCGG_T
Cuts at: 2689 2689
Size: 4301
AleI CACnn'nnGTG
Cuts at: 254 254
Size: 4301
AlwNI CAG_nnn'CTG
Cuts at: 621 1606 3173 621
Size: 985 1567 1749
Fragments arranged by size:
1749 1567 985
ApaLI G'TGCA_C
Cuts at: 3071 3071
Size: 4301
ApoI r'AATT_y
Cuts at: 1183 1305 3654 3910 1183
Size: 122 2349 256 1574
Fragments arranged by size:
2349 1574 256 122
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 294 317 1321 294
Size: 23 1004 3274
Fragments arranged by size:
3274 1004 23
BamHI G'GATC_C
Cuts at: 1326 1326
Size: 4301
BanI G'GyrC_C
Cuts at: 759 1317 1731 1979 2014 759
Size: 558 414 248 35 3046
Fragments arranged by size:
3046 558 414 248 35
BanII G_rGCy'C
Cuts at: 318 854 1315 2345 318
Size: 536 461 1030 2274
Fragments arranged by size:
2274 1030 536 461
BbeI G_GCGC'C
Cuts at: 1735 1983 1735
Size: 248 4053
BbsI GAAGACnn'nnnn_
Cuts at: 156 156
Size: 4301
BciVI GTATCCnnnnn_n'
Cuts at: 877 2199 2960 4301 877
Size: 1322 761 1341 877
Fragments arranged by size:
1341 1322 877 761
BclI T'GATC_A
Cuts at: 990 1821 990
Size: 831 3470
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4301
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 1413 1413
Size: 4301
Bme1580I G_kGCm'C
Cuts at: 764 801 1926 2019 3075 764
Size: 37 1125 93 1056 1990
Fragments arranged by size:
1990 1125 1056 93 37
BmgBI CAC'GTC
Cuts at: 168 168
Size: 4301
BmrI ACTGGGnnnn_n'
Cuts at: 964 1513 1594 1929 4073 964
Size: 549 81 335 2144 1192
Fragments arranged by size:
2144 1192 549 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4301
Bpu10I CC'TnA_GC
Cuts at: 629 1104 629
Size: 475 3826
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1477 2356 2848 3146 826
Size: 651 879 492 298 1981
Fragments arranged by size:
1981 879 651 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4005 4005
Size: 4301
BsaAI yAC'GTr
Cuts at: 865 1654 865
Size: 789 3512
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 885 1732 1980 2682 4178 885
Size: 847 248 702 1496 1008
Fragments arranged by size:
1496 1008 847 702 248
BsaWI w'CCGG_w
Cuts at: 243 253 1490 2011 2689 2963 3110 3521 243
Size: 10 1237 521 678 274 147 411 1023
Fragments arranged by size:
1237 1023 678 521 411 274 147 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 335 371 1282 1285 1288 335
Size: 36 911 3 3 3348
Fragments arranged by size:
3348 911 36 3 3
BseYI C'CCAG_C
Cuts at: 1726 2484 3061 3557 1726
Size: 758 577 496 2470
Fragments arranged by size:
2470 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1843 1889 3097 104
Size: 1739 46 1208 1308
Fragments arranged by size:
1739 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1315 2093 2283 3075 1315
Size: 778 190 792 2541
Fragments arranged by size:
2541 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 211 272 892 211
Size: 61 620 3620
Fragments arranged by size:
3620 620 61
Bsp1286I G_dGCh'C
Cuts at: 318 764 801 854 1315 1926 2019 2093 2283
Size: 446 37 53 461 611 93 74 190
Cuts at: 2283 2345 3075 318
Size: 62 730 1544
Fragments arranged by size:
1544 730 611 461 446 190 93 74
62 53 37
BspEI T'CCGG_A
Cuts at: 243 3521 243
Size: 3278 1023
BspHI T'CATG_A
Cuts at: 3477 3477
Size: 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 629 1351 1565 1867 2248 629
Size: 722 214 302 381 2682
Fragments arranged by size:
2682 722 381 302 214
BsrBI CCG'CTC
Cuts at: 50 293 642 2593 2647 4131 4296 50
Size: 243 349 1951 54 1484 165 55
Fragments arranged by size:
1951 1484 349 243 165 55 54
BsrDI GCAATG_nn'
Cuts at: 971 1116 2213 971
Size: 145 1097 3059
Fragments arranged by size:
3059 1097 145
BsrFI r'CCGG_y
Cuts at: 143 177 285 329 2299 2480 2689 143
Size: 34 108 44 1970 181 209 1755
Fragments arranged by size:
1970 1755 209 181 108 44 34
BsrGI T'GTAC_A
Cuts at: 926 926
Size: 4301
BssSI C'ACGA_G
Cuts at: 2572 2930 2572
Size: 358 3943
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 2662 2662
Size: 4301
BstXI CCAn_nnnn'nTGG
Cuts at: 256 256
Size: 4301
BstYI r'GATC_y
Cuts at: 1 1326 1793 1816 2151 2397 3409 3495 3507
Size: 1325 467 23 335 246 1012 86 12
Cuts at: 3507 3853 1
Size: 346 449
Fragments arranged by size:
1325 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 360 360
Size: 4301
BtgI C'CryG_G
Cuts at: 303 303
Size: 4301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 658 2251 2432 2595 658
Size: 1593 181 163 2364
Fragments arranged by size:
2364 1593 181 163
BtsI GCAGTG_nn'
Cuts at: 711 1544 711
Size: 833 3468
DraI TTT'AAA
Cuts at: 900 1300 900
Size: 400 3901
DrdI GACnn_nn'nnGTC
Cuts at: 2007 2865 2007
Size: 858 3443
EaeI y'GGCC_r
Cuts at: 1068 1886 2060 2451 2478 3823 1068
Size: 818 174 391 27 1345 1546
Fragments arranged by size:
1546 1345 818 391 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 115 632 1068 2324 2534 115
Size: 517 436 1256 210 1882
Fragments arranged by size:
1882 1256 517 436 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 260 2509 2819 2965 4111 260
Size: 2249 310 146 1146 450
Fragments arranged by size:
2249 1146 450 310 146
EcoICRI GAG'CTC
Cuts at: 1313 1313
Size: 4301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 2558 3305 3573 2126
Size: 432 747 268 2854
Fragments arranged by size:
2854 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1440 1440
Size: 4301
EcoRI G'AATT_C
Cuts at: 1305 1305
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 4301
FspI TGC'GCA
Cuts at: 2082 2082
Size: 4301
HaeII r_GCGC'y
Cuts at: 1735 1983 3005 3984 1735
Size: 248 1022 979 2052
Fragments arranged by size:
2052 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 982 1014 1087 1119 1193 1225 2143 2173 2175
Size: 32 73 32 74 32 918 30 2
Cuts at: 2175 2205 982
Size: 30 3078
Fragments arranged by size:
3078 918 74 73 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 961 1340 961
Size: 379 3922
HindIII A'AGCT_T
Cuts at: 780 780
Size: 4301
KasI G'GCGC_C
Cuts at: 1731 1979 1731
Size: 248 4053
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MfeI C'AATT_G
Cuts at: 986 986
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1208 2972 3156 1208
Size: 1764 184 2353
Fragments arranged by size:
2353 1764 184
MscI TGG'CCA
Cuts at: 2062 2062
Size: 4301
MslI CAynn'nnrTG
Cuts at: 254 2290 2417 254
Size: 2036 127 2138
Fragments arranged by size:
2138 2036 127
MspA1I CmG'CkG
Cuts at: 305 1412 1726 2086 3099 3344 305
Size: 1107 314 360 1013 245 1262
Fragments arranged by size:
1262 1107 1013 360 314 245
NaeI GCC'GGC
Cuts at: 145 179 287 2482 145
Size: 34 108 2195 1964
Fragments arranged by size:
2195 1964 108 34
NarI GG'CG_CC
Cuts at: 1732 1980 1732
Size: 248 4053
NgoMIV G'CCGG_C
Cuts at: 143 177 285 2480 143
Size: 34 108 2195 1964
Fragments arranged by size:
2195 1964 108 34
NspI r_CATG'y
Cuts at: 1018 1354 1854 2761 1018
Size: 336 500 907 2558
Fragments arranged by size:
2558 907 500 336
PciI A'CATG_T
Cuts at: 2757 2757
Size: 4301
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 4269
PsiI TTA'TAA
Cuts at: 653 653
Size: 4301
PstI C_TGCA'G
Cuts at: 626 1348 2033 626
Size: 722 685 2894
Fragments arranged by size:
2894 722 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 1726 2086 1726
Size: 360 3941
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 1315 1315
Size: 4301
SacII CC_GC'GG
Cuts at: 306 306
Size: 4301
SalI G'TCGA_C
Cuts at: 959 1338 959
Size: 379 3922
SapI GCTCTTCn'nnn_
Cuts at: 115 632 2324 2534 115
Size: 517 1692 210 1882
Fragments arranged by size:
1882 1692 517 210
SbfI CC_TGCA'GG
Cuts at: 626 1348 626
Size: 722 3579
ScaI AGT'ACT
Cuts at: 3818 3818
Size: 4301
SfcI C'TryA_G
Cuts at: 33 622 1344 2029 3022 3213 33
Size: 589 722 685 993 191 1121
Fragments arranged by size:
1121 993 722 685 589 191
SfoI GGC'GCC
Cuts at: 1733 1981 1733
Size: 248 4053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 1323 1323
Size: 4301
SmlI C'TyrA_G
Cuts at: 805 1456 2371 2863 3125 805
Size: 651 915 492 262 1981
Fragments arranged by size:
1981 915 651 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1018 1354 1854 1018
Size: 336 500 3465
Fragments arranged by size:
3465 500 336
StuI AGG'CCT
Cuts at: 358 576 358
Size: 218 4083
StyI C'CwwG_G
Cuts at: 1098 1435 1789 1098
Size: 337 354 3610
Fragments arranged by size:
3610 354 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 354 1884 2017 2476 2685 354
Size: 1530 133 459 209 1970
Fragments arranged by size:
1970 1530 459 209 133
TatI w'GTAC_w
Cuts at: 926 1176 3816 926
Size: 250 2640 1411
Fragments arranged by size:
2640 1411 250
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4269
Tth111I GACn'n_nGTC
Cuts at: 2098 2098
Size: 4301
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1796 1796
Size: 4301
XmaI C'CCGG_G
Cuts at: 1321 1321
Size: 4301
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI
BsaXI BsgI BsiWI BsmI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI