Restriction map for pF1KE0349
 (Linear) (Six-base) MAPSORT of: KIEE0349.seq  Check: 2261  from: 1  to: 2154



KIEE0349.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     797    1777    1793    1820    2154 
   Size:        797     980      16      27     334

  Fragments arranged by size: 

                 980     797     334      27      16
 
AfeI AGC'GCT

Cuts at:      0    1390    2154 
   Size:       1390     764
 
AflIII A'CryG_T

Cuts at:      0     650    2154 
   Size:        650    1504
 
AlwNI CAG_nnn'CTG

Cuts at:      0     211     567    2154 
   Size:        211     356    1587

  Fragments arranged by size: 

                1587     356     211
 
ApoI r'AATT_y

Cuts at:      0     247     708    2126    2154 
   Size:        247     461    1418      28

  Fragments arranged by size: 

                1418     461     247      28
 
AvaI C'yCGr_G

Cuts at:      0    1211    2154 
   Size:       1211     943
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     813     846    2154 
   Size:        813      33    1308

  Fragments arranged by size: 

                1308     813      33
 
BanI G'GyrC_C

Cuts at:      0     154     436    1275    2154 
   Size:        154     282     839     879

  Fragments arranged by size: 

                 879     839     282     154
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1781    2154 
   Size:       1781     373
 
BbvCI CC'TCA_GC

Cuts at:      0    2005    2154 
   Size:       2005     149
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     106     956    2154 
   Size:        106     850    1198

  Fragments arranged by size: 

                1198     850     106
 
BclI T'GATC_A

Cuts at:      0     575    1259    2154 
   Size:        575     684     895

  Fragments arranged by size: 

                 895     684     575
 
BglI GCCn_nnn'nGGC

Cuts at:      0      37    2154 
   Size:         37    2117
 
Bme1580I G_kGCm'C

Cuts at:      0     441     588    1479    2154 
   Size:        441     147     891     675

  Fragments arranged by size: 

                 891     675     441     147
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1345    1837    2154 
   Size:       1345     492     317

  Fragments arranged by size: 

                1345     492     317
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1689    2154 
   Size:       1689     465
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     233    2154 
   Size:        233    1921
 
Bpu10I CC'TnA_GC

Cuts at:      0    2005    2154 
   Size:       2005     149
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1732    2018    2154 
   Size:       1732     286     136

  Fragments arranged by size: 

                1732     286     136
 
BsaBI GATnn'nnATC

Cuts at:      0    1808    2154 
   Size:       1808     346
 
BsaWI w'CCGG_w

Cuts at:      0     305     523    2154 
   Size:        305     218    1631

  Fragments arranged by size: 

                1631     305     218
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     518     548    1587    1617    1876    1906    2154 
   Size:        518      30    1039      30     259      30     248

  Fragments arranged by size: 

                1039     518     259     248      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1846    2028    2053    2154 
   Size:       1846     182      25     101

  Fragments arranged by size: 

                1846     182     101      25
 
BseYI C'CCAG_C

Cuts at:      0     807    2154 
   Size:        807    1347
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     868    2154 
   Size:        868    1286
 
BsiHKAI G_wGCw'C

Cuts at:      0     191    1004    2154 
   Size:        191     813    1150

  Fragments arranged by size: 

                1150     813     191
 
BsmI GAATG_Cn'

Cuts at:      0    1712    2154 
   Size:       1712     442
 
Bsp1286I G_dGCh'C

Cuts at:      0     191     441     588    1004    1479    2154 
   Size:        191     250     147     416     475     675

  Fragments arranged by size: 

                 675     475     416     250     191     147
 
BspHI T'CATG_A

Cuts at:      0     572    2154 
   Size:        572    1582
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1831    2154 
   Size:       1831     323
 
BsrDI GCAATG_nn'

Cuts at:      0      26     872    1401    2154 
   Size:         26     846     529     753

  Fragments arranged by size: 

                 846     753     529      26
 
BsrGI T'GTAC_A

Cuts at:      0    1181    2154 
   Size:       1181     973
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      28     567    1008    2154 
   Size:         28     539     441    1146

  Fragments arranged by size: 

                1146     539     441      28
 
BstBI TT'CG_AA

Cuts at:      0    1623    2154 
   Size:       1623     531
 
BstEII G'GTnAC_C

Cuts at:      0    1818    2154 
   Size:       1818     336
 
BstYI r'GATC_y

Cuts at:      0     208     423    2154 
   Size:        208     215    1731

  Fragments arranged by size: 

                1731     215     208
 
Bsu36I CC'TnA_GG

Cuts at:      0    1854    2154 
   Size:       1854     300
 
BtsI GCAGTG_nn'

Cuts at:      0     239     545    1002    1112    1731    2154 
   Size:        239     306     457     110     619     423

  Fragments arranged by size: 

                 619     457     423     306     239     110
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1268    1303    2154 
   Size:       1268      35     851

  Fragments arranged by size: 

                1268     851      35
 
DraI TTT'AAA

Cuts at:      0    1992    2154 
   Size:       1992     162
 
EaeI y'GGCC_r

Cuts at:      0     354    1541    2154 
   Size:        354    1187     613

  Fragments arranged by size: 

                1187     613     354
 
EarI CTCTTCn'nnn_

Cuts at:      0    2030    2060    2154 
   Size:       2030      30      94

  Fragments arranged by size: 

                2030      94      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     233     797    1777    1793    1820    2154 
   Size:        233     564     980      16      27     334

  Fragments arranged by size: 

                 980     564     334     233      27      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     855    1788    2154 
   Size:        855     933     366

  Fragments arranged by size: 

                 933     855     366
 
EcoRI G'AATT_C

Cuts at:      0     247    2154 
   Size:        247    1907
 
HaeII r_GCGC'y

Cuts at:      0     132    1392    2154 
   Size:        132    1260     762

  Fragments arranged by size: 

                1260     762     132
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1104    1123    1136    1155    1876    1908    2154 
   Size:       1104      19      13      19     721      32     246

  Fragments arranged by size: 

                1104     721     246      32      19      19      13
 
HincII GTy'rAC

Cuts at:      0    1884    2154 
   Size:       1884     270
 
MfeI C'AATT_G

Cuts at:      0     149    2154 
   Size:        149    2005
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     725     784    2154 
   Size:        445     280      59    1370

  Fragments arranged by size: 

                1370     445     280      59
 
MscI TGG'CCA

Cuts at:      0     356    1543    2154 
   Size:        356    1187     611

  Fragments arranged by size: 

                1187     611     356
 
MslI CAynn'nnrTG

Cuts at:      0     499    1012    2154 
   Size:        499     513    1142

  Fragments arranged by size: 

                1142     513     499
 
NspI r_CATG'y

Cuts at:      0     654    1011    2154 
   Size:        654     357    1143

  Fragments arranged by size: 

                1143     654     357
 
PciI A'CATG_T

Cuts at:      0     650    2154 
   Size:        650    1504
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1637    2154 
   Size:       1637     517
 
PfoI T'CCnGG_A

Cuts at:      0     449     958    2154 
   Size:        449     509    1196

  Fragments arranged by size: 

                1196     509     449
 
PpuMI rG'GwC_Cy

Cuts at:      0     855    2154 
   Size:        855    1299
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1951    1983    2154 
   Size:       1951      32     171

  Fragments arranged by size: 

                1951     171      32
 
PstI C_TGCA'G

Cuts at:      0     563    1828    2154 
   Size:        563    1265     326

  Fragments arranged by size: 

                1265     563     326
 
SexAI A'CCwGG_T

Cuts at:      0    1652    2154 
   Size:       1652     502
 
SfcI C'TryA_G

Cuts at:      0     559    1824    2154 
   Size:        559    1265     330

  Fragments arranged by size: 

                1265     559     330
 
SmaI CCC'GGG

Cuts at:      0    1213    2154 
   Size:       1213     941
 
SphI G_CATG'C

Cuts at:      0    1011    2154 
   Size:       1011    1143
 
SspI AAT'ATT

Cuts at:      0     177    1044    2097    2154 
   Size:        177     867    1053      57

  Fragments arranged by size: 

                1053     867     177      57
 
StuI AGG'CCT

Cuts at:      0     530    2154 
   Size:        530    1624
 
StyI C'CwwG_G

Cuts at:      0     778    2154 
   Size:        778    1376
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1263    1906    2154 
   Size:       1263     643     248

  Fragments arranged by size: 

                1263     643     248
 
TatI w'GTAC_w

Cuts at:      0       9    1181    2154 
   Size:          9    1172     973

  Fragments arranged by size: 

                1172     973       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     218     250    1587    1619    2154 
   Size:        218      32    1337      32     535

  Fragments arranged by size: 

                1337     535     218      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     838    2154 
   Size:        838    1316
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     417     815    2154 
   Size:        417     398    1339

  Fragments arranged by size: 

                1339     417     398
 
XmaI C'CCGG_G

Cuts at:      0    1211    2154 
   Size:       1211     943
 
XmnI GAAnn'nnTTC

Cuts at:      0     773     954    1619    2154 
   Size:        773     181     665     535

  Fragments arranged by size: 

                 773     665     535     181
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII    AlwNI     ApoI     AvaI     BaeI     BanI
     BbsI    BbvCI    BciVI     BclI     BglI Bme1580I     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtsI    CspCI     DraI
     EaeI     EarI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I   HincII
     MfeI     MmeI     MscI     MslI     NspI     PciI    PflMI     PfoI
    PpuMI     PsrI     PstI    SexAI     SfcI     SmaI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII    BamHI    BanII     BbeI     BcgI    BfrBI    BglII     BlpI
    BmgBI     BmtI    BpuEI    BsaAI    BsaHI    BsiEI    BsiWI    BsmBI
    BspEI    BsrBI    BsrFI   BssHII    BssSI    BstXI  BstZ17I     BtgI
    BtgZI     ClaI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI     SwaI
     XbaI     XhoI     ZraI
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