Restriction map for pF1KB6428
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        ApaLI       
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BcgI        BciVI       BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoO65I     EcoT22I     
FokI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
Ksp632I     MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SpeI        SphI        
Sse8387I    SspBI       SwaI        TspRI       Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AfaI          453  GTAC                 GT^AC
Aor51HI       810  AGCGCT               AGC^GCT
ApaI          391  GGGCCC               GGGCC^C
AscI          207  GGCGCGCC             GG^CGCGCC
AvaII          91  GGACC                G^G[AT]CC
BanII         391  GGGCCC               G[GA]GC[CT]^C
BbvCI         239  CCTCAGC              CCTCAGC(-5/-2)
BclI          155  TGATCA               T^GATCA
BcnI          164  CCGGG                CC^[GC]GG
BglII         603  AGATCT               A^GATCT
BmyI          391  GGGCCC               G[AGT]GC[ACT]^C
BpmI          860  CTGGAG               CTGGAG(16/14)
BseRI         185  GAGGAG               GAGGAG(10/8)
BsmAI         863  GAGAC                GTCTC(1/5)
BsmFI         468  GGGAC                GGGAC(10/14)
Bsp1286I      391  GGGCCC               G[AGT]GC[ACT]^C
BssHII        208  GCGCGC               G^CGCGC
BstYI         603  AGATCT               [GA]^GATC[CT]
DraII         391  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          583  CCGCGG               C^C[GA][CT]GG
Eco47III      810  AGCGCT               AGC^GCT
EcoNI         395  CCTTGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      391  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI          64  GAATTC               G^AATTC
EcoRV         547  GATATC               GAT^ATC
FauI          194  GCGGG                CCCGC(4/6)
FbaI          155  TGATCA               T^GATCA
HaeII         810  AGCGCT               [GA]GCGC^[CT]
HapII         164  CCGG                 C^CGG
HindIII       335  AAGCTT               A^AGCTT
HpaII         164  CCGG                 C^CGG
KspI          583  CCGCGG               CCGC^GG
MflI          603  AGATCT               [GA]^GATC[CT]
MseI           58  TTAA                 T^TAA
MslI          798  CATAGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          164  CCGG                 C^CGG
NciI          164  CCGGG                CC^[GC]GG
NlaIII        963  CATG                 CATG^
PleI          470  GACTC                GAGTC(4/5)
PvuII         760  CAGCTG               CAG^CTG
RsaI          453  GTAC                 GT^AC
SacII         583  CCGCGG               CCGC^GG
SfcI          329  CTGTAG               C^T[GA][CT]AG
SmlI          593  CTCAAG               C^T[CT][GA]AG
SnaBI         955  TACGTA               TAC^GTA
SspI           54  AATATT               AAT^ATT
StuI          135  AGGCCT               AGG^CCT
TaqI          924  TCGA                 T^CGA
Tru9I          58  TTAA                 T^TAA
TthHB8I       924  TCGA                 T^CGA
XhoII         603  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII         209  CGCG                 CG^CG
              584  CGCG                
BbsI          445  GTCTTC               GAAGAC(2/6)
              696  GTCTTC              
BpuAI         445  GTCTTC               GAAGAC(2/6)
              696  GTCTTC              
BsgI          233  CTGCAC               GTGCAG(16/14)
              749  CTGCAC              
BsiYI         362  CCCTCGGAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              395  CCTTGGGAAGG         
BslI          362  CCCTCGGAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              395  CCTTGGGAAGG         
BsrI          934  ACTGG                ACTGG(1/-1)
              754  CCAGT               
BstNI         171  CCTGG                CC^[AT]GG
              838  CCTGG               
BstUI         209  CGCG                 CG^CG
              584  CGCG                
DdeI          240  CTCAG                C^T[ACGT]AG
              427  CTGAG               
EcoRII        171  CCTGG                ^CC[AT]GG
              838  CCTGG               
EcoT14I       261  CCTTGG               C^C[AT][AT]GG
              395  CCTTGG              
HphI          174  GGTGA                GGTGA(8/7)
              303  GGTGA               
MvaI          171  CCTGG                CC^[AT]GG
              838  CCTGG               
MvnI          209  CGCG                 CG^CG
              584  CGCG                
NlaIV         167  GGTTCC               GG[ACGT]^[ACGT]CC
              391  GGGCCC              
StyI          261  CCTTGG               C^C[AT][AT]GG
              395  CCTTGG              
Tsp45I        175  GTGAC                ^GT[GC]AC
              805  GTGAC               
XcmI          217  CCAGATGGAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              759  CCAGCTGAACTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BbvI        
BsaAI       BsaJI       BssKI       Cac8I       CfoI        
Cfr13I      DpnII       Fnu4HI      HaeIII      HhaI        
HinP1I      HinfI       ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MspA1I      MwoI        
NdeII       Sau3AI      Sau96I      ScrFI       TfiI        
TseI        Tsp509I     
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