Restriction map for pF1KB6428
 (Linear) (Six-base) MAPSORT of: KIBB6428.seq  Check: 9038  from: 1  to: 969



KIBB6428.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AfeI AGC'GCT

Cuts at:      0     812     969 
   Size:        812     157
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     764     969 
   Size:        764     205
 
ApaI G_GGCC'C

Cuts at:      0     395     969 
   Size:        395     574
 
ApoI r'AATT_y

Cuts at:      0      64     532     614     849     969 
   Size:         64     468      82     235     120

  Fragments arranged by size: 

                 468     235     120      82      64
 
AscI GG'CGCG_CC

Cuts at:      0     208     969 
   Size:        208     761
 
BanII G_rGCy'C

Cuts at:      0     395     969 
   Size:        395     574
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     438     689     969 
   Size:        438     251     280

  Fragments arranged by size: 

                 438     280     251
 
BbvCI CC'TCA_GC

Cuts at:      0     240     969 
   Size:        240     729
 
BclI T'GATC_A

Cuts at:      0     155     969 
   Size:        155     814
 
BglII A'GATC_T

Cuts at:      0     603     969 
   Size:        603     366
 
Bme1580I G_kGCm'C

Cuts at:      0     395     969 
   Size:        395     574
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     881     969 
   Size:        881      88
 
Bpu10I CC'TnA_GC

Cuts at:      0     240     969 
   Size:        240     729
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     969 
   Size:        578     391
 
BsaAI yAC'GTr

Cuts at:      0     117     452     957     969 
   Size:        117     335     505      12

  Fragments arranged by size: 

                 505     335     117      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     200     969 
   Size:        200     769
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     734     969 
   Size:        218     516     235

  Fragments arranged by size: 

                 516     235     218
 
Bsp1286I G_dGCh'C

Cuts at:      0     395     969 
   Size:        395     574
 
BssHII G'CGCG_C

Cuts at:      0     208     969 
   Size:        208     761
 
BstYI r'GATC_y

Cuts at:      0     603     969 
   Size:        603     366
 
BtgI C'CryG_G

Cuts at:      0     583     969 
   Size:        583     386
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     365     969 
   Size:        365     604
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     881     969 
   Size:        881      88
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     399     969 
   Size:        399     570
 
EcoO109I rG'GnC_Cy

Cuts at:      0     392     969 
   Size:        392     577
 
EcoRI G'AATT_C

Cuts at:      0      64     969 
   Size:         64     905
 
EcoRV GAT'ATC

Cuts at:      0     549     969 
   Size:        549     420
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     937     969 
   Size:        937      32
 
HaeII r_GCGC'y

Cuts at:      0     814     969 
   Size:        814     155
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     644     676     854     886     969 
   Size:        644      32     178      32      83

  Fragments arranged by size: 

                 644     178      83      32      32
 
HindIII A'AGCT_T

Cuts at:      0     335     969 
   Size:        335     634
 
MslI CAynn'nnrTG

Cuts at:      0     802     969 
   Size:        802     167
 
MspA1I CmG'CkG

Cuts at:      0     194     439     585     762     969 
   Size:        194     245     146     177     207

  Fragments arranged by size: 

                 245     207     194     177     146
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     757     789     969 
   Size:        757      32     180

  Fragments arranged by size: 

                 757     180      32
 
PspOMI G'GGCC_C

Cuts at:      0     391     969 
   Size:        391     578
 
PvuII CAG'CTG

Cuts at:      0     762     969 
   Size:        762     207
 
SacII CC_GC'GG

Cuts at:      0     586     969 
   Size:        586     383
 
SfcI C'TryA_G

Cuts at:      0     329     969 
   Size:        329     640
 
SmlI C'TyrA_G

Cuts at:      0     593     969 
   Size:        593     376
 
SnaBI TAC'GTA

Cuts at:      0     957     969 
   Size:        957      12
 
SspI AAT'ATT

Cuts at:      0      56     969 
   Size:         56     913
 
StuI AGG'CCT

Cuts at:      0     137     969 
   Size:        137     832
 
StyI C'CwwG_G

Cuts at:      0     261     395     969 
   Size:        261     134     574

  Fragments arranged by size: 

                 574     261     134
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     224     766     969 
   Size:        224     542     203

  Fragments arranged by size: 

                 542     224     203
 

 Enzymes that do cut:

     AfeI     AlfI     ApaI     ApoI     AscI    BanII     BbsI    BbvCI
     BclI    BglII Bme1580I     BpmI   Bpu10I    BpuEI    BsaAI    BseRI
     BsgI Bsp1286I   BssHII    BstYI     BtgI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I  HindIII     MslI
   MspA1I     PpiI   PspOMI    PvuII    SacII     SfcI     SmlI    SnaBI
     SspI     StuI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SpeI     SphI     SrfI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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