Restriction map for
pF1KE0364
(Circular) (Six-base) MAPSORT of: pF1KE0364.seq Check: 334 from: 1 to: 5183
pF1KE0364 5183 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1815 1815
Size: 5183
AccI GT'mk_AC
Cuts at: 1400 2221 1400
Size: 821 4362
Acc65I G'GTAC_C
Cuts at: 2199 2199
Size: 5183
AclI AA'CG_TT
Cuts at: 5023 5023
Size: 5183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 604 3008 3440 4187 4455 433
Size: 171 2404 432 747 268 1161
Fragments arranged by size:
2404 1161 747 432 268 171
AfeI AGC'GCT
Cuts at: 1372 1372
Size: 5183
AflIII A'CryG_T
Cuts at: 229 1790 1850 2535 3639 4616 229
Size: 1561 60 685 1104 977 796
Fragments arranged by size:
1561 1104 977 796 685 60
AgeI A'CCGG_T
Cuts at: 3571 3571
Size: 5183
AlwNI CAG_nnn'CTG
Cuts at: 190 937 2488 4055 190
Size: 747 1551 1567 1318
Fragments arranged by size:
1567 1551 1318 747
ApaI G_GGCC'C
Cuts at: 582 774 1458 2115 582
Size: 192 684 657 3650
Fragments arranged by size:
3650 684 657 192
ApaLI G'TGCA_C
Cuts at: 3953 3953
Size: 5183
ApoI r'AATT_y
Cuts at: 2187 4536 4792 2187
Size: 2349 256 2578
Fragments arranged by size:
2578 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5183
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5183
AvaI C'yCGr_G
Cuts at: 557 773 993 1640 1759 2203 557
Size: 216 220 647 119 444 3537
Fragments arranged by size:
3537 647 444 220 216 119
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1260 1293 1260
Size: 33 5150
BamHI G'GATC_C
Cuts at: 222 1142 2208 222
Size: 920 1066 3197
Fragments arranged by size:
3197 1066 920
BanI G'GyrC_C
Cuts at: 285 2199 2613 2861 2896 285
Size: 1914 414 248 35 2572
Fragments arranged by size:
2572 1914 414 248 35
BanII G_rGCy'C
Cuts at: 582 766 774 897 1262 1458 2115 2197 3227
Size: 184 8 123 365 196 657 82 1030
Cuts at: 3227 582
Size: 2538
Fragments arranged by size:
2538 1030 657 365 196 184 123 82
8
BbeI G_GCGC'C
Cuts at: 2617 2865 2617
Size: 248 4935
BbsI GAAGACnn'nnnn_
Cuts at: 2047 2047
Size: 5183
BbvCI CC'TCA_GC
Cuts at: 862 862
Size: 5183
BciVI GTATCCnnnnn_n'
Cuts at: 3081 3842 5183 3081
Size: 761 1341 3081
Fragments arranged by size:
3081 1341 761
BclI T'GATC_A
Cuts at: 2703 2703
Size: 5183
BglI GCCn_nnn'nGGC
Cuts at: 180 294 342 568 586 180
Size: 114 48 226 18 4777
Fragments arranged by size:
4777 226 114 48 18
BglII A'GATC_T
Cuts at: 1158 2698 4735 1158
Size: 1540 2037 1606
Fragments arranged by size:
2037 1606 1540
BlpI GC'TnA_GC
Cuts at: 298 590 760 2295 298
Size: 292 170 1535 3186
Fragments arranged by size:
3186 1535 292 170
Bme1580I G_kGCm'C
Cuts at: 290 501 582 774 1458 2115 2808 2901 3957
Size: 211 81 192 684 657 693 93 1056
Cuts at: 3957 290
Size: 1516
Fragments arranged by size:
1516 1056 693 684 657 211 192 93
81
BmrI ACTGGGnnnn_n'
Cuts at: 925 2109 2395 2476 2811 4955 925
Size: 1184 286 81 335 2144 1153
Fragments arranged by size:
2144 1184 1153 335 286 81
BmtI G_CTAG'C
Cuts at: 1912 1912
Size: 5183
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3550 3550
Size: 5183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 2190 832
Size: 1358 3825
Bpu10I CC'TnA_GC
Cuts at: 862 862
Size: 5183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 450 2359 3238 3730 4028 450
Size: 1909 879 492 298 1605
Fragments arranged by size:
1909 1605 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1351 4887 1351
Size: 3536 1647
BsaAI yAC'GTr
Cuts at: 1122 2536 1122
Size: 1414 3769
BsaBI GATnn'nnATC
Cuts at: 6 2697 4734 6
Size: 2691 2037 455
Fragments arranged by size:
2691 2037 455
BsaHI Gr'CG_yC
Cuts at: 2006 2614 2862 3564 5060 2006
Size: 608 248 702 1496 2129
Fragments arranged by size:
2129 1496 702 608 248
BsaWI w'CCGG_w
Cuts at: 1301 2372 2893 3571 3845 3992 4403 1301
Size: 1071 521 678 274 147 411 2081
Fragments arranged by size:
2081 1071 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 314 344 1801 1831 1927 1957 314
Size: 30 1457 30 96 30 3540
Fragments arranged by size:
3540 1457 96 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 829 1468 829
Size: 639 4544
BseYI C'CCAG_C
Cuts at: 594 681 696 952 1286 2608 3366 3943 4439
Size: 87 15 256 334 1322 758 577 496
Cuts at: 4439 594
Size: 1338
Fragments arranged by size:
1338 1322 758 577 496 334 256 87
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1528 1924 1528
Size: 396 4787
BsiEI CG_ry'CG
Cuts at: 104 1755 2725 2771 3979 104
Size: 1651 970 46 1208 1308
Fragments arranged by size:
1651 1308 1208 970 46
BsiHKAI G_wGCw'C
Cuts at: 480 766 1262 2197 2975 3165 3957 480
Size: 286 496 935 778 190 792 1706
Fragments arranged by size:
1706 935 792 778 496 286 190
BsmI GAATG_Cn'
Cuts at: 1490 1490
Size: 5183
Bsp1286I G_dGCh'C
Cuts at: 290 480 501 582 766 774 897 1262 1458
Size: 190 21 81 184 8 123 365 196
Cuts at: 1458 2115 2197 2808 2901 2975 3165 3227 3957
Size: 657 82 611 93 74 190 62 730
Cuts at: 3957 290
Size: 1516
Fragments arranged by size:
1516 730 657 611 365 196 190 190
184 123 93 82 81 74 62 21
8
BspEI T'CCGG_A
Cuts at: 4403 4403
Size: 5183
BspHI T'CATG_A
Cuts at: 4359 4359
Size: 5183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 953 1815 2233 2447 2749 3130 953
Size: 862 418 214 302 381 3006
Fragments arranged by size:
3006 862 418 381 302 214
BsrBI CCG'CTC
Cuts at: 50 471 1038 3475 3529 5013 5178 50
Size: 421 567 2437 54 1484 165 55
Fragments arranged by size:
2437 1484 567 421 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1331 1338 3095 1331
Size: 7 1757 3419
Fragments arranged by size:
3419 1757 7
BsrFI r'CCGG_y
Cuts at: 369 434 526 3181 3362 3571 369
Size: 65 92 2655 181 209 1981
Fragments arranged by size:
2655 1981 209 181 92 65
BssSI C'ACGA_G
Cuts at: 479 1801 3454 3812 479
Size: 1322 1653 358 1850
Fragments arranged by size:
1850 1653 1322 358
BstAPI GCAn_nnn'nTGC
Cuts at: 756 2560 756
Size: 1804 3379
BstBI TT'CG_AA
Cuts at: 3544 3544
Size: 5183
BstEII G'GTnAC_C
Cuts at: 598 2016 598
Size: 1418 3765
BstXI CCAn_nnnn'nTGG
Cuts at: 713 713
Size: 5183
BstYI r'GATC_y
Cuts at: 1 222 395 818 1142 1158 1388 2208 2675
Size: 221 173 423 324 16 230 820 467
Cuts at: 2675 2698 3033 3279 4291 4377 4389 4735 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 820 467 449 423 346 335 324
246 230 221 173 86 23 16 12
BtgI C'CryG_G
Cuts at: 107 291 1632 1721 2009 107
Size: 184 1341 89 288 3281
Fragments arranged by size:
3281 1341 288 184 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3133 3314 3477 3133
Size: 181 163 4839
Fragments arranged by size:
4839 181 163
BtsI GCAGTG_nn'
Cuts at: 2426 2426
Size: 5183
DraI TTT'AAA
Cuts at: 2182 2182
Size: 5183
DraIII CAC_nnn'GTG
Cuts at: 756 933 1806 756
Size: 177 873 4133
Fragments arranged by size:
4133 873 177
DrdI GACnn_nn'nnGTC
Cuts at: 2889 3747 2889
Size: 858 4325
EaeI y'GGCC_r
Cuts at: 181 251 389 689 729 1718 1917 2768 2942
Size: 70 138 300 40 989 199 851 174
Cuts at: 2942 3333 3360 4705 181
Size: 391 27 1345 659
Fragments arranged by size:
1345 989 851 659 391 300 199 174
138 70 40 27
EagI C'GGCC_G
Cuts at: 2768 2768
Size: 5183
EarI CTCTTCn'nnn_
Cuts at: 985 3206 3416 985
Size: 2221 210 2752
Fragments arranged by size:
2752 2221 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1306 1543 3391 3701 3847 4993 1306
Size: 237 1848 310 146 1146 1496
Fragments arranged by size:
1848 1496 1146 310 237 146
EcoICRI GAG'CTC
Cuts at: 764 1260 2195 764
Size: 496 935 3752
Fragments arranged by size:
3752 935 496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 604 832 2190 3008 3440 4187 4455 433
Size: 171 228 1358 818 432 747 268 1161
Fragments arranged by size:
1358 1161 818 747 432 268 228 171
EcoNI CCTnn'n_nnAGG
Cuts at: 881 881
Size: 5183
EcoO109I rG'GnC_Cy
Cuts at: 162 517 579 770 1454 1455 2322 162
Size: 355 62 191 684 1 867 3023
Fragments arranged by size:
3023 867 684 355 191 62 1
EcoRI G'AATT_C
Cuts at: 2187 2187
Size: 5183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3202 3202
Size: 5183
FspI TGC'GCA
Cuts at: 1669 2964 1669
Size: 1295 3888
HaeII r_GCGC'y
Cuts at: 922 1374 1449 2617 2865 3887 4866 922
Size: 452 75 1168 248 1022 979 1239
Fragments arranged by size:
1239 1168 1022 979 452 248 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1530 1562 1777 1809 3025 3055 3057 3087 1530
Size: 32 215 32 1216 30 2 30 3626
Fragments arranged by size:
3626 1216 215 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1434 1848 2085 2222 1434
Size: 414 237 137 4395
Fragments arranged by size:
4395 414 237 137
HpaI GTT'AAC
Cuts at: 1848 1848
Size: 5183
KasI G'GCGC_C
Cuts at: 2613 2861 2613
Size: 248 4935
KpnI G_GTAC'C
Cuts at: 2203 2203
Size: 5183
MluI A'CGCG_T
Cuts at: 4616 4616
Size: 5183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1631 3854 4038 1631
Size: 2223 184 2776
Fragments arranged by size:
2776 2223 184
MscI TGG'CCA
Cuts at: 183 253 391 691 1720 2944 183
Size: 70 138 300 1029 1224 2422
Fragments arranged by size:
2422 1224 1029 300 138 70
MslI CAynn'nnrTG
Cuts at: 3172 3299 3172
Size: 127 5056
MspA1I CmG'CkG
Cuts at: 310 851 1084 1634 2294 2608 2968 3981 4226
Size: 541 233 550 660 314 360 1013 245
Cuts at: 4226 310
Size: 1267
Fragments arranged by size:
1267 1013 660 550 541 360 314 245
233
NaeI GCC'GGC
Cuts at: 371 436 528 3364 371
Size: 65 92 2836 2190
Fragments arranged by size:
2836 2190 92 65
NarI GG'CG_CC
Cuts at: 2614 2862 2614
Size: 248 4935
NcoI C'CATG_G
Cuts at: 107 291 1721 2009 107
Size: 184 1430 288 3281
Fragments arranged by size:
3281 1430 288 184
NgoMIV G'CCGG_C
Cuts at: 369 434 526 3362 369
Size: 65 92 2836 2190
Fragments arranged by size:
2836 2190 92 65
NheI G'CTAG_C
Cuts at: 1908 1908
Size: 5183
NspI r_CATG'y
Cuts at: 233 2236 2736 3643 233
Size: 2003 500 907 1773
Fragments arranged by size:
2003 1773 907 500
PasI CC'CwG_GG
Cuts at: 281 1458 281
Size: 1177 4006
PciI A'CATG_T
Cuts at: 229 3639 229
Size: 3410 1773
PflMI CCAn_nnn'nTGG
Cuts at: 1125 1125
Size: 5183
PfoI T'CCnGG_A
Cuts at: 800 2000 800
Size: 1200 3983
PmeI GTTT'AAAC
Cuts at: 2182 2182
Size: 5183
PmlI CAC'GTG
Cuts at: 1122 1122
Size: 5183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4322 4354 4322
Size: 32 5151
PspOMI G'GGCC_C
Cuts at: 578 770 1454 2111 578
Size: 192 684 657 3650
Fragments arranged by size:
3650 684 657 192
PstI C_TGCA'G
Cuts at: 836 1049 2230 2915 836
Size: 213 1181 685 3104
Fragments arranged by size:
3104 1181 685 213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5183
PvuII CAG'CTG
Cuts at: 310 1084 2608 2968 310
Size: 774 1524 360 2525
Fragments arranged by size:
2525 1524 774 360
RsrII CG'GwC_CG
Cuts at: 3378 3378
Size: 5183
SacI G_AGCT'C
Cuts at: 766 1262 2197 766
Size: 496 935 3752
Fragments arranged by size:
3752 935 496
SacII CC_GC'GG
Cuts at: 1635 1635
Size: 5183
SalI G'TCGA_C
Cuts at: 2220 2220
Size: 5183
SapI GCTCTTCn'nnn_
Cuts at: 3206 3416 3206
Size: 210 4973
SbfI CC_TGCA'GG
Cuts at: 2230 2230
Size: 5183
ScaI AGT'ACT
Cuts at: 4700 4700
Size: 5183
SfcI C'TryA_G
Cuts at: 33 832 1045 1565 2226 2911 3904 4095 33
Size: 799 213 520 661 685 993 191 1121
Fragments arranged by size:
1121 993 799 685 661 520 213 191
SfoI GGC'GCC
Cuts at: 2615 2863 2615
Size: 248 4935
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5183
SmaI CCC'GGG
Cuts at: 559 775 2205 559
Size: 216 1430 3537
Fragments arranged by size:
3537 1430 216
SmlI C'TyrA_G
Cuts at: 429 2338 3253 3745 4007 429
Size: 1909 915 492 262 1605
Fragments arranged by size:
1909 1605 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5183
SphI G_CATG'C
Cuts at: 2236 2736 2236
Size: 500 4683
SrfI GCCC'GGGC
Cuts at: 559 559
Size: 5183
StyI C'CwwG_G
Cuts at: 107 291 1598 1721 2009 2317 2671 107
Size: 184 1307 123 288 308 354 2619
Fragments arranged by size:
2619 1307 354 308 288 184 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 273 586 944 1366 2766 2899 3358 3567
Size: 173 313 358 422 1400 133 459 209
Cuts at: 3567 100
Size: 1716
Fragments arranged by size:
1716 1400 459 422 358 313 209 173
133
TatI w'GTAC_w
Cuts at: 2097 4698 2097
Size: 2601 2582
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 314 346 1298 1330 4700 4732 314
Size: 32 952 32 3370 32 765
Fragments arranged by size:
3370 952 765 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2980 2980
Size: 5183
XbaI T'CTAG_A
Cuts at: 2214 2214
Size: 5183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 359 1176 1803 2678 359
Size: 817 627 875 2864
Fragments arranged by size:
2864 875 817 627
XmaI C'CCGG_G
Cuts at: 557 773 2203 557
Size: 216 1430 3537
Fragments arranged by size:
3537 1430 216
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BcgI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII MfeI
NdeI NotI NruI NsiI PacI PpuMI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI ZraI