################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AhdI AlwI AscI AseI AsnI Asp700I AspEI AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstYI BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KspI MaeI MamI MfeI MflI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 1053 GGTACC G^GTACC AccII 893 CGCG CG^CG AcsI 348 GAATTT [GA]^AATT[CT] AcyI 520 GGCGTC G[GA]^CG[CT]C Alw44I 125 GTGCAC G^TGCAC AocI 1474 CCTGAGG CC^T[ACGT]AGG Aor51HI 1110 AGCGCT AGC^GCT ApaLI 125 GTGCAC G^TGCAC ApoI 348 GAATTT [GA]^AATT[CT] Asp718I 1053 GGTACC G^GTACC AspI 1349 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BlpI 1071 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1071 GCTGAGC GC^T[ACGT]AGC BsaHI 520 GGCGTC G[GA]^CG[CT]C BsaI 181 GAGACC GGTCTC(1/5) BseRI 1367 CTCCTC GAGGAG(10/8) BsmFI 1180 GGGAC GGGAC(10/14) BsmI 1149 GCATTC GAATGC(1/-1) BstAPI 1433 GCACAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 352 TTCGAA TT^CGAA BstUI 893 CGCG CG^CG BstXI 284 CCATGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1474 CCTGAGG CC^T[ACGT]AGG CelII 1071 GCTGAGC GC^T[ACGT]AGC DpnII 1223 GATC ^GATC DraIII 730 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1110 AGCGCT AGC^GCT Eco81I 1474 CCTGAGG CC^T[ACGT]AGG HgaI 521 GCGTC GACGC(5/10) Hin1I 520 GGCGTC G[GA]^CG[CT]C KpnI 1053 GGTACC GGTAC^C MboI 1223 GATC ^GATC MspA1I 1551 CAGCTG C[AC]G^C[GT]G MvnI 893 CGCG CG^CG NdeII 1223 GATC ^GATC NspV 352 TTCGAA TT^CGAA PflFI 1349 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 482 AGGACCT [GA]G^G[AT]CC[CT] PstI 1226 CTGCAG CTGCA^G PvuII 1551 CAGCTG CAG^CTG Sau3AI 1223 GATC ^GATC SfuI 352 TTCGAA TT^CGAA SmlI 760 CTCAAG C^T[CT][GA]AG TaqI 353 TCGA T^CGA Tth111I 1349 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 353 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AvaI 704 CCCGGG C^[CT]CG[GA]G 1253 CCCGGG AviII 865 TGCGCA TGC^GCA 974 TGCGCA BalI 657 TGGCCA TGG^CCA 677 TGGCCA BsmAI 181 GAGAC GTCTC(1/5) 1122 GAGAC BsoBI 704 CCCGGG C^[CT]CG[GA]G 1253 CCCGGG BstEII 816 GGTGACC G^GT[ACGT]ACC 948 GGTGACC BstPI 816 GGTGACC G^GT[ACGT]ACC 948 GGTGACC DsaI 852 CCATGG C^C[GA][CT]GG 959 CCATGG EaeI 657 TGGCCA [CT]^GGCC[GA] 677 TGGCCA EcoNI 222 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1470 CCTACCTGAGG EcoO65I 816 GGTGACC G^GT[ACGT]ACC 948 GGTGACC FspI 865 TGCGCA TGC^GCA 974 TGCGCA HaeII 662 AGCGCC [GA]GCGC^[CT] 1110 AGCGCT MluNI 657 TGGCCA TGG^CCA 677 TGGCCA MscI 657 TGGCCA TGG^CCA 677 TGGCCA MseI 83 TTAA T^TAA 208 TTAA MslI 285 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1194 CACCCTTGTG NcoI 852 CCATGG C^CATGG 959 CCATGG NspI 548 GCATGT [GA]CATG^[CT] 1438 ACATGC PleI 333 GACTC GAGTC(4/5) 1404 GACTC SacI 685 GAGCTC GAGCT^C 1382 GAGCTC SapI 687 GCTCTTC GCTCTTC(1/4) 1205 GCTCTTC SfcI 752 CTACAG C^T[GA][CT]AG 1226 CTGCAG SmaI 704 CCCGGG CCC^GGG 1253 CCCGGG Tru9I 83 TTAA T^TAA 208 TTAA Tsp45I 817 GTGAC ^GT[GC]AC 949 GTGAC XmaCI 704 CCCGGG C^CCGGG 1253 CCCGGG XmaI 704 CCCGGG C^CCGGG 1253 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AspHI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PflMI RsaI Sau96I ScrFI SfaNI StyI TfiI TseI Tsp509I TspRI Van91I XcmI