Restriction map for pF1KB8939
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbrPI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    EarI        Eco47III    Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
ScaI        SexAI       SfaNI       SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp45I      Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI          340  AAATTC               [GA]^AATT[CT]
AfaI          426  GTAC                 GT^AC
AlwI          204  GATCC                GGATC(4/5)
ApaI          750  GGGCCC               GGGCC^C
ApoI          340  AAATTC               [GA]^AATT[CT]
BbeI          310  GGCGCC               GGCGC^C
BbsI          645  GAAGAC               GAAGAC(2/6)
BbvCI         696  CCTCAGC              CCTCAGC(-5/-2)
BpmI          208  CTGGAG               CTGGAG(16/14)
BpuAI         645  GAAGAC               GAAGAC(2/6)
BsaI          376  GAGACC               GGTCTC(1/5)
BsgI          727  CTGCAC               GTGCAG(16/14)
BsmAI         376  GAGAC                GTCTC(1/5)
BssSI         856  CTCGTG               CACGAG(-5/-1)
BstYI         203  AGATCC               [GA]^GATC[CT]
DdeI          697  CTCAG                C^T[ACGT]AG
DpnII         204  GATC                 ^GATC
DraII         750  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      750  GGGCCCC              [GA]G^G[ACGT]CC[CT]
HaeII         310  GGCGCC               [GA]GCGC^[CT]
HgaI          660  GACGC                GACGC(5/10)
KasI          310  GGCGCC               G^GCGCC
KspI          177  CCGCGG               CCGC^GG
MboI          204  GATC                 ^GATC
MboII         645  GAAGA                GAAGA(8/7)
MflI          203  AGATCC               [GA]^GATC[CT]
NarI          310  GGCGCC               GG^CGCC
NcoI          813  CCATGG               C^CATGG
NdeII         204  GATC                 ^GATC
PvuII         253  CAGCTG               CAG^CTG
RsaI          426  GTAC                 GT^AC
SacI          193  GAGCTC               GAGCT^C
SacII         177  CCGCGG               CCGC^GG
Sau3AI        204  GATC                 ^GATC
SbfI          819  CCTGCAGG             CCTGCA^GG
SfiI          159  GGCCGTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          310  GGCGCC               GGC^GCC
Sse8387I      819  CCTGCAGG             CCTGCA^GG
XcmI          155  CCAAGGCCGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         203  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          310  GGCGCC               G[GA]^CG[CT]C
              660  GACGCC              
AvaII         795  GGACC                G^G[AT]CC
              861  GGACC               
BanI          310  GGCGCC               G^G[CT][GA]CC
              774  GGTGCC              
BcnI          537  CCGGG                CC^[GC]GG
              748  CCGGG               
BglI          160  GCCGTGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              708  GCCCCCTGGGC         
BsaHI         310  GGCGCC               G[GA]^CG[CT]C
              660  GACGCC              
BseRI         694  CTCCTC               GAGGAG(10/8)
              373  GAGGAG              
BsrBI          82  GAGCGG               CCGCTC(-3/-3)
              791  GAGCGG              
BsrFI         521  ACCGGC               [GA]^CCGG[CT]
              771  GCCGGT              
BstXI         155  CCAAGGCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              851  CCAACCTCGTGG        
Cfr10I        521  ACCGGC               [GA]^CCGG[CT]
              771  GCCGGT              
EaeI          297  CGGCCG               [CT]^GGCC[GA]
              542  CGGCCG              
EagI          297  CGGCCG               C^GGCCG
              542  CGGCCG              
EclXI         297  CGGCCG               C^GGCCG
              542  CGGCCG              
Eco52I        297  CGGCCG               C^GGCCG
              542  CGGCCG              
Hin1I         310  GGCGCC               G[GA]^CG[CT]C
              660  GACGCC              
HphI          448  TCACC                GGTGA(8/7)
              488  TCACC               
MslI          130  CACAAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              808  CACCACCATG          
NciI          537  CCGGG                CC^[GC]GG
              748  CCGGG               
SmlI          148  CTCAAG               C^T[CT][GA]AG
              262  CTCAAG              
TaqI           80  TCGA                 T^CGA
              278  TCGA                
Tsp509I       341  AATT                 ^AATT
              580  AATT                
TthHB8I        80  TCGA                 T^CGA
              278  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AspHI       
BanII       BbvI        BmyI        BsaJI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DsaI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
ItaI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NlaIII      NlaIV       PleI        
PstI        Sau96I      ScrFI       SfcI        StyI        
TseI        TspRI       
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