Restriction map for pF1KB6534
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbsI        
BbvCI       BcgI        BciVI       BfaI        BfrI        
BglI        BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
FauI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        KasI        KpnI        MaeI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccII         961  CGCG                 CG^CG
AfaI          679  GTAC                 GT^AC
AlwI         1057  GGATC                GGATC(4/5)
AlwNI        1022  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BalI          601  TGGCCA               TGG^CCA
BbrPI         833  CACGTG               CAC^GTG
BclI          177  TGATCA               T^GATCA
BcnI          953  CCGGG                CC^[GC]GG
BglII         717  AGATCT               A^GATCT
BsaAI         833  CACGTG               [CT]AC^GT[GA]
BseRI        1012  GAGGAG               GAGGAG(10/8)
BsgI          146  CTGCAC               GTGCAG(16/14)
Bsp1407I      678  TGTACA               T^GTACA
BspDI         751  ATCGAT               AT^CGAT
BsrGI         678  TGTACA               T^GTACA
BssSI         967  CTCGTG               CACGAG(-5/-1)
BstAPI       1021  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         961  CGCG                 CG^CG
BstYI         717  AGATCT               [GA]^GATC[CT]
ClaI          751  ATCGAT               AT^CGAT
DraII         689  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII          8  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          601  TGGCCA               [CT]^GGCC[GA]
EcoNI         984  CCTACACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      689  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         916  GATATC               GAT^ATC
EcoT22I       290  ATGCAT               ATGCA^T
FbaI          177  TGATCA               T^GATCA
HapII         953  CCGG                 C^CGG
HpaII         953  CCGG                 C^CGG
KspI          960  CCGCGG               CCGC^GG
MflI          717  AGATCT               [GA]^GATC[CT]
MluNI         601  TGGCCA               TGG^CCA
MscI          601  TGGCCA               TGG^CCA
MspI          953  CCGG                 C^CGG
MvnI          961  CGCG                 CG^CG
NciI          953  CCGGG                CC^[GC]GG
NcoI          902  CCATGG               C^CATGG
NdeI          293  CATATG               CA^TATG
NsiI          290  ATGCAT               ATGCA^T
PleI          980  GAGTC                GAGTC(4/5)
PmaCI         833  CACGTG               CAC^GTG
PmlI          833  CACGTG               CAC^GTG
PpuMI         689  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         831  GACACGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1028  CTGCAG               CTGCA^G
PvuII        1031  CAGCTG               CAG^CTG
RsaI          679  GTAC                 GT^AC
SacII         960  CCGCGG               CCGC^GG
SapI          318  GCTCTTC              GCTCTTC(1/4)
SmlI          883  CTCAAG               C^T[CT][GA]AG
SspBI         678  TGTACA               T^GTACA
StuI          992  AGGCCT               AGG^CCT
Tsp45I        184  GTCAC                ^GT[GC]AC
XcmI          902  CCATGGGAATGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         717  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AvaII         120  GGTCC                G^G[AT]CC
              690  GGTCC               
BsaBI        1052  GATTCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1157  GATTCTCATC          
BsaI          757  GGTCTC               GGTCTC(1/5)
              964  GGTCTC              
BsiYI         703  CCATACAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              984  CCTACACAAGG         
BslI          703  CCATACAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              984  CCTACACAAGG         
BsrI          249  CCAGT                ACTGG(1/-1)
              386  CCAGT               
BstNI         446  CCTGG                CC^[AT]GG
              693  CCTGG               
BstXI         686  CCAAGGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              902  CCATGGGAATGG        
Cfr13I        120  GGTCC                G^G[ACGT]CC
              690  GGTCC               
DsaI          902  CCATGG               C^C[GA][CT]GG
              960  CCGCGG              
EcoRI           3  GAATTC               G^AATTC
              271  GAATTC              
EcoRII        446  CCTGG                ^CC[AT]GG
              693  CCTGG               
EcoT14I       686  CCAAGG               C^C[AT][AT]GG
              902  CCATGG              
HindIII       259  AAGCTT               A^AGCTT
              766  AAGCTT              
MaeIII        184  GTCAC                ^GT[ACGT]AC
             1006  GTTAC               
MamI         1052  GATTCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1157  GATTCTCATC          
MvaI          446  CCTGG                CC^[AT]GG
              693  CCTGG               
NlaIV         130  GGAGCC               GG[ACGT]^[ACGT]CC
              956  GGAGCC              
Sau96I        120  GGTCC                G^G[ACGT]CC
              690  GGTCC               
SfcI         1028  CTGCAG               C^T[GA][CT]AG
             1043  CTGTAG              
SspI          353  AATATT               AAT^ATT
              499  AATATT              
StyI          686  CCAAGG               C^C[AT][AT]GG
              902  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AcsI        AflIII      AluI        ApoI        
BbvI        BpmI        BsaJI       BsmAI       BssKI       
BstF5I      DdeI        DpnII       EarI        Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
Ksp632I     MaeII       MboI        MboII       MnlI        
MseI        MslI        MspA1I      MwoI        NdeII       
NlaIII      Sau3AI      ScrFI       SfaNI       TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     
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