Restriction map for
pF1KE0223
(Circular) (Six-base) MAPSORT of: pF1KE0223.seq Check: 5768 from: 1 to: 3350
pF1KE0223 3350 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 388 388
Size: 3350
Acc65I G'GTAC_C
Cuts at: 366 366
Size: 3350
AclI AA'CG_TT
Cuts at: 3190 3190
Size: 3350
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 223 1175 1607 2354 2622 157
Size: 66 952 432 747 268 885
Fragments arranged by size:
952 885 747 432 268 66
AflIII A'CryG_T
Cuts at: 702 1806 2783 702
Size: 1104 977 1269
Fragments arranged by size:
1269 1104 977
AgeI A'CCGG_T
Cuts at: 1738 1738
Size: 3350
AhdI GACnn_n'nnGTC
Cuts at: 186 186
Size: 3350
AlwNI CAG_nnn'CTG
Cuts at: 655 2222 655
Size: 1567 1783
ApaLI G'TGCA_C
Cuts at: 2120 2120
Size: 3350
ApoI r'AATT_y
Cuts at: 223 313 354 2703 2959 223
Size: 90 41 2349 256 614
Fragments arranged by size:
2349 614 256 90 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 3350
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3350
AvaI C'yCGr_G
Cuts at: 370 370
Size: 3350
BamHI G'GATC_C
Cuts at: 375 375
Size: 3350
BanI G'GyrC_C
Cuts at: 366 780 1028 1063 366
Size: 414 248 35 2653
Fragments arranged by size:
2653 414 248 35
BanII G_rGCy'C
Cuts at: 364 1394 364
Size: 1030 2320
BbeI G_GCGC'C
Cuts at: 784 1032 784
Size: 248 3102
BciVI GTATCCnnnnn_n'
Cuts at: 1248 2009 3350 1248
Size: 761 1341 1248
Fragments arranged by size:
1341 1248 761
BclI T'GATC_A
Cuts at: 870 870
Size: 3350
BglII A'GATC_T
Cuts at: 865 2902 865
Size: 2037 1313
BlpI GC'TnA_GC
Cuts at: 462 462
Size: 3350
Bme1580I G_kGCm'C
Cuts at: 975 1068 2124 975
Size: 93 1056 2201
Fragments arranged by size:
2201 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 224 562 643 978 3122 224
Size: 338 81 335 2144 452
Fragments arranged by size:
2144 452 338 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1717 1717
Size: 3350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1405 1897 2195 526
Size: 879 492 298 1681
Fragments arranged by size:
1681 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3054 3054
Size: 3350
BsaAI yAC'GTr
Cuts at: 703 703
Size: 3350
BsaBI GATnn'nnATC
Cuts at: 6 864 2901 6
Size: 858 2037 455
Fragments arranged by size:
2037 858 455
BsaHI Gr'CG_yC
Cuts at: 781 1029 1731 3227 781
Size: 248 702 1496 904
Fragments arranged by size:
1496 904 702 248
BsaWI w'CCGG_w
Cuts at: 539 1060 1738 2012 2159 2570 539
Size: 521 678 274 147 411 1319
Fragments arranged by size:
1319 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 775 1533 2110 2606 775
Size: 758 577 496 1519
Fragments arranged by size:
1519 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 892 938 2146 104
Size: 788 46 1208 1308
Fragments arranged by size:
1308 1208 788 46
BsiHKAI G_wGCw'C
Cuts at: 364 1142 1332 2124 364
Size: 778 190 792 1590
Fragments arranged by size:
1590 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 364 975 1068 1142 1332 1394 2124 364
Size: 611 93 74 190 62 730 1590
Fragments arranged by size:
1590 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2570 2570
Size: 3350
BspHI T'CATG_A
Cuts at: 2526 2526
Size: 3350
BspMI ACCTGCnnnn'nnnn_
Cuts at: 400 614 916 1297 400
Size: 214 302 381 2453
Fragments arranged by size:
2453 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1642 1696 3180 3345 50
Size: 1592 54 1484 165 55
Fragments arranged by size:
1592 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1262 1262
Size: 3350
BsrFI r'CCGG_y
Cuts at: 1348 1529 1738 1348
Size: 181 209 2960
Fragments arranged by size:
2960 209 181
BssSI C'ACGA_G
Cuts at: 1621 1979 1621
Size: 358 2992
BstAPI GCAn_nnn'nTGC
Cuts at: 727 727
Size: 3350
BstBI TT'CG_AA
Cuts at: 1711 1711
Size: 3350
BstYI r'GATC_y
Cuts at: 1 375 842 865 1200 1446 2458 2544 2556
Size: 374 467 23 335 246 1012 86 12
Cuts at: 2556 2902 1
Size: 346 449
Fragments arranged by size:
1012 467 449 374 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 128 128
Size: 3350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1300 1481 1644 1300
Size: 181 163 3006
Fragments arranged by size:
3006 181 163
BtsI GCAGTG_nn'
Cuts at: 593 593
Size: 3350
DraI TTT'AAA
Cuts at: 349 349
Size: 3350
DrdI GACnn_nn'nnGTC
Cuts at: 1056 1914 1056
Size: 858 2492
EaeI y'GGCC_r
Cuts at: 935 1109 1500 1527 2872 935
Size: 174 391 27 1345 1413
Fragments arranged by size:
1413 1345 391 174 27
EagI C'GGCC_G
Cuts at: 935 935
Size: 3350
EarI CTCTTCn'nnn_
Cuts at: 133 1373 1583 133
Size: 1240 210 1900
Fragments arranged by size:
1900 1240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1558 1868 2014 3160 1558
Size: 310 146 1146 1748
Fragments arranged by size:
1748 1146 310 146
EcoICRI GAG'CTC
Cuts at: 362 362
Size: 3350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 223 1175 1607 2354 2622 157
Size: 66 952 432 747 268 885
Fragments arranged by size:
952 885 747 432 268 66
EcoO109I rG'GnC_Cy
Cuts at: 489 489
Size: 3350
EcoRI G'AATT_C
Cuts at: 223 313 354 223
Size: 90 41 3219
Fragments arranged by size:
3219 90 41
EcoRV GAT'ATC
Cuts at: 338 338
Size: 3350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1369 1369
Size: 3350
FspI TGC'GCA
Cuts at: 1131 1131
Size: 3350
HaeII r_GCGC'y
Cuts at: 784 1032 2054 3033 784
Size: 248 1022 979 1101
Fragments arranged by size:
1101 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 172 204 1192 1222 1224 1254 172
Size: 32 988 30 2 30 2268
Fragments arranged by size:
2268 988 32 30 30 2
HincII GTy'rAC
Cuts at: 389 389
Size: 3350
KasI G'GCGC_C
Cuts at: 780 1028 780
Size: 248 3102
KpnI G_GTAC'C
Cuts at: 370 370
Size: 3350
MluI A'CGCG_T
Cuts at: 2783 2783
Size: 3350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2021 2205 2021
Size: 184 3166
MscI TGG'CCA
Cuts at: 1111 1111
Size: 3350
MslI CAynn'nnrTG
Cuts at: 1339 1466 1339
Size: 127 3223
MspA1I CmG'CkG
Cuts at: 461 775 1135 2148 2393 461
Size: 314 360 1013 245 1418
Fragments arranged by size:
1418 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1531 1531
Size: 3350
NarI GG'CG_CC
Cuts at: 781 1029 781
Size: 248 3102
NgoMIV G'CCGG_C
Cuts at: 1529 1529
Size: 3350
NspI r_CATG'y
Cuts at: 403 903 1810 403
Size: 500 907 1943
Fragments arranged by size:
1943 907 500
PciI A'CATG_T
Cuts at: 1806 1806
Size: 3350
PmeI GTTT'AAAC
Cuts at: 349 349
Size: 3350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2489 2521 2489
Size: 32 3318
PstI C_TGCA'G
Cuts at: 397 1082 397
Size: 685 2665
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3350
PvuII CAG'CTG
Cuts at: 775 1135 775
Size: 360 2990
RsrII CG'GwC_CG
Cuts at: 1545 1545
Size: 3350
SacI G_AGCT'C
Cuts at: 364 364
Size: 3350
SalI G'TCGA_C
Cuts at: 387 387
Size: 3350
SapI GCTCTTCn'nnn_
Cuts at: 1373 1583 1373
Size: 210 3140
SbfI CC_TGCA'GG
Cuts at: 397 397
Size: 3350
ScaI AGT'ACT
Cuts at: 114 2867 114
Size: 2753 597
SfcI C'TryA_G
Cuts at: 33 393 1078 2071 2262 33
Size: 360 685 993 191 1121
Fragments arranged by size:
1121 993 685 360 191
SfoI GGC'GCC
Cuts at: 782 1030 782
Size: 248 3102
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3350
SmaI CCC'GGG
Cuts at: 372 372
Size: 3350
SmlI C'TyrA_G
Cuts at: 505 1420 1912 2174 505
Size: 915 492 262 1681
Fragments arranged by size:
1681 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3350
SphI G_CATG'C
Cuts at: 403 903 403
Size: 500 2850
StyI C'CwwG_G
Cuts at: 484 838 484
Size: 354 2996
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 933 1066 1525 1734 933
Size: 133 459 209 2549
Fragments arranged by size:
2549 459 209 133
TatI w'GTAC_w
Cuts at: 112 2865 112
Size: 2753 597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 3318
Tth111I GACn'n_nGTC
Cuts at: 1147 1147
Size: 3350
XbaI T'CTAG_A
Cuts at: 381 381
Size: 3350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 845 845
Size: 3350
XmaI C'CCGG_G
Cuts at: 370 370
Size: 3350
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI