Restriction map for pF1KE0409
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BpmI        BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MunI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TseI        Tth111I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          191  GTCTAC               GT^[AC][GT]AC
AccII          39  CGCG                 CG^CG
AcsI          107  GAATTC               [GA]^AATT[CT]
AfaI          380  GTAC                 GT^AC
AlwNI         353  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          230  GGGCCC               GGGCC^C
ApoI          107  GAATTC               [GA]^AATT[CT]
AspHI          22  GAGCTC               G[AT]GC[AT]^C
BanI          573  GGTGCC               G^G[CT][GA]CC
BbsI          509  GAAGAC               GAAGAC(2/6)
BbvCI         342  CCTCAGC              CCTCAGC(-5/-2)
BlpI           72  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       72  GCTGAGC              GC^T[ACGT]AGC
BpuAI         509  GAAGAC               GAAGAC(2/6)
BseRI          19  GAGGAG               GAGGAG(10/8)
BsgI           13  GTGCAG               GTGCAG(16/14)
BsiHKAI        22  GAGCTC               G[AT]GC[AT]^C
BsmFI          94  GGGAC                GGGAC(10/14)
BspHI         300  TCATGA               T^CATGA
BsrBI          78  CCGCTC               CCGCTC(-3/-3)
BssSI          58  CACGAG               CACGAG(-5/-1)
BstUI          39  CGCG                 CG^CG
CelII          72  GCTGAGC              GC^T[ACGT]AGC
DpnII         439  GATC                 ^GATC
DsaI          401  CCATGG               C^C[GA][CT]GG
EaeI           35  TGGCCG               [CT]^GGCC[GA]
EcoRI         107  GAATTC               G^AATTC
EcoT14I       401  CCATGG               C^C[AT][AT]GG
HincII        222  GTTGAC               GT[CT]^[GA]AC
HindII        222  GTTGAC               GT[CT]^[GA]AC
MboI          439  GATC                 ^GATC
MboII         509  GAAGA                GAAGA(8/7)
MslI          396  CACGACCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           39  CGCG                 CG^CG
MwoI           15  GCAGGAGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          401  CCATGG               C^CATGG
NdeII         439  GATC                 ^GATC
PflMI         352  CCAGAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI           48  CTGCAG               CTGCA^G
RcaI          300  TCATGA               T^CATGA
RsaI          380  GTAC                 GT^AC
SacI           22  GAGCTC               GAGCT^C
Sau3AI        439  GATC                 ^GATC
SexAI         195  ACCTGGT              A^CC[AT]GGT
SfaNI         136  GATGC                GCATC(5/9)
StyI          401  CCATGG               C^C[AT][AT]GG
TaqI          205  TCGA                 T^CGA
Tsp45I        241  GTGAC                ^GT[GC]AC
TthHB8I       205  TCGA                 T^CGA
Van91I        352  CCAGAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          348  CCAACCAGAAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AvaII          30  GGTCC                G^G[AT]CC
               95  GGACC               
BanII          22  GAGCTC               G[GA]GC[CT]^C
              230  GGGCCC              
BmyI           22  GAGCTC               G[AGT]GC[ACT]^C
              230  GGGCCC              
BsaJI         227  CCAGGG               C^C[ACGT][ACGT]GG
              401  CCATGG              
BsmAI          85  GAGAC                GTCTC(1/5)
              554  GAGAC               
Bsp1286I       22  GAGCTC               G[AGT]GC[ACT]^C
              230  GGGCCC              
BstF5I        135  GGATG                GGATG(2/0)
              405  GGATG               
Cac8I          50  GCAGGC               GC[ACGT]^[ACGT]GC
              267  GCAAGC              
DraII          52  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              229  AGGGCCC             
EcoO109I       52  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              229  AGGGCCC             
Fnu4HI         37  GCCGC                GC^[ACGT]GC
               77  GCCGC               
FokI          135  GGATG                GGATG(9/13)
              405  GGATG               
HinfI         318  GATTC                G^A[ACGT]TC
              327  GAATC               
ItaI           37  GCCGC                GC^[ACGT]GC
               77  GCCGC               
MaeIII        241  GTGAC                ^GT[ACGT]AC
              258  GTTAC               
MseI          209  TTAA                 T^TAA
              496  TTAA                
SfcI           48  CTGCAG               C^T[GA][CT]AG
              547  CTACAG              
SmlI          262  CTTGAG               C^T[CT][GA]AG
              568  CTCAAG              
TfiI          318  GATTC                G^A[AT]TC
              327  GAATC               
Tru9I         209  TTAA                 T^TAA
              496  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AciI        AluI        BsiYI       BslI        BsrI        
BssKI       BstNI       Cfr13I      DdeI        EcoRII      
HaeIII      MnlI        MvaI        NlaIII      NlaIV       
Sau96I      ScrFI       Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com