Restriction map for
pF1KB8936
(Circular) (Six-base) MAPSORT of: pF1KB8936.seq Check: 3375 from: 1 to: 3968
pF1KB8936 3968 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 935 935
Size: 3968
AccI GT'mk_AC
Cuts at: 1006 1006
Size: 3968
Acc65I G'GTAC_C
Cuts at: 984 984
Size: 3968
AclI AA'CG_TT
Cuts at: 3808 3808
Size: 3968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 1793 2225 2972 3240 255
Size: 1538 432 747 268 983
Fragments arranged by size:
1538 983 747 432 268
AflII C'TTAA_G
Cuts at: 940 940
Size: 3968
AflIII A'CryG_T
Cuts at: 240 800 1320 2424 3401 240
Size: 560 520 1104 977 807
Fragments arranged by size:
1104 977 807 560 520
AgeI A'CCGG_T
Cuts at: 2356 2356
Size: 3968
AlwNI CAG_nnn'CTG
Cuts at: 867 1273 2840 867
Size: 406 1567 1995
Fragments arranged by size:
1995 1567 406
ApaI G_GGCC'C
Cuts at: 751 751
Size: 3968
ApaLI G'TGCA_C
Cuts at: 140 193 898 2738 140
Size: 53 705 1840 1370
Fragments arranged by size:
1840 1370 705 53
ApoI r'AATT_y
Cuts at: 972 3321 3577 972
Size: 2349 256 1363
Fragments arranged by size:
2349 1363 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3968
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
AvaI C'yCGr_G
Cuts at: 154 291 397 476 572 750 758 838 988
Size: 137 106 79 96 178 8 80 150
Cuts at: 988 154
Size: 3134
Fragments arranged by size:
3134 178 150 137 106 96 80 79
8
BamHI G'GATC_C
Cuts at: 993 993
Size: 3968
BanI G'GyrC_C
Cuts at: 199 984 1398 1646 1681 199
Size: 785 414 248 35 2486
Fragments arranged by size:
2486 785 414 248 35
BanII G_rGCy'C
Cuts at: 128 170 608 751 923 982 2012 128
Size: 42 438 143 172 59 1030 2084
Fragments arranged by size:
2084 1030 438 172 143 59 42
BbeI G_GCGC'C
Cuts at: 1402 1650 1402
Size: 248 3720
BbsI GAAGACnn'nnnn_
Cuts at: 294 435 294
Size: 141 3827
BciVI GTATCCnnnnn_n'
Cuts at: 1866 2627 3968 1866
Size: 761 1341 1866
Fragments arranged by size:
1866 1341 761
BclI T'GATC_A
Cuts at: 1488 1488
Size: 3968
BglI GCCn_nnn'nGGC
Cuts at: 385 587 704 385
Size: 202 117 3649
Fragments arranged by size:
3649 202 117
BglII A'GATC_T
Cuts at: 1483 3520 1483
Size: 2037 1931
BlpI GC'TnA_GC
Cuts at: 1080 1080
Size: 3968
Bme1580I G_kGCm'C
Cuts at: 144 197 476 751 902 1593 1686 2742 144
Size: 53 279 275 151 691 93 1056 1370
Fragments arranged by size:
1370 1056 691 279 275 151 93 53
BmgBI CAC'GTC
Cuts at: 801 801
Size: 3968
BmrI ACTGGGnnnn_n'
Cuts at: 1180 1261 1596 3740 1180
Size: 81 335 2144 1408
Fragments arranged by size:
2144 1408 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 3968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 2023 2515 2813 1144
Size: 879 492 298 2299
Fragments arranged by size:
2299 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3672 3672
Size: 3968
BsaAI yAC'GTr
Cuts at: 1321 1321
Size: 3968
BsaBI GATnn'nnATC
Cuts at: 6 1482 3519 6
Size: 1476 2037 455
Fragments arranged by size:
2037 1476 455
BsaHI Gr'CG_yC
Cuts at: 1399 1647 2349 3845 1399
Size: 248 702 1496 1522
Fragments arranged by size:
1522 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1157 1678 2356 2630 2777 3188 1157
Size: 521 678 274 147 411 1937
Fragments arranged by size:
1937 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 520 334
Size: 186 3782
BseYI C'CCAG_C
Cuts at: 330 511 1393 2151 2728 3224 330
Size: 181 882 758 577 496 1074
Fragments arranged by size:
1074 882 758 577 496 181
BsiEI CG_ry'CG
Cuts at: 104 659 713 1510 1556 2764 104
Size: 555 54 797 46 1208 1308
Fragments arranged by size:
1308 1208 797 555 54 46
BsiHKAI G_wGCw'C
Cuts at: 144 197 902 982 1760 1950 2742 144
Size: 53 705 80 778 190 792 1370
Fragments arranged by size:
1370 792 778 705 190 80 53
BsmI GAATG_Cn'
Cuts at: 566 566
Size: 3968
BsmBI CGTCTCn'nnnn_
Cuts at: 358 358
Size: 3968
Bsp1286I G_dGCh'C
Cuts at: 128 144 170 197 476 608 751 902 923
Size: 16 26 27 279 132 143 151 21
Cuts at: 923 982 1593 1686 1760 1950 2012 2742 128
Size: 59 611 93 74 190 62 730 1354
Fragments arranged by size:
1354 730 611 279 190 151 143 132
93 74 62 59 27 26 21 16
BspEI T'CCGG_A
Cuts at: 3188 3188
Size: 3968
BspHI T'CATG_A
Cuts at: 3144 3144
Size: 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 935 1018 1232 1534 1915 935
Size: 83 214 302 381 2988
Fragments arranged by size:
2988 381 302 214 83
BsrBI CCG'CTC
Cuts at: 50 2260 2314 3798 3963 50
Size: 2210 54 1484 165 55
Fragments arranged by size:
2210 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1880 1880
Size: 3968
BsrFI r'CCGG_y
Cuts at: 596 902 1966 2147 2356 596
Size: 306 1064 181 209 2208
Fragments arranged by size:
2208 1064 306 209 181
BssHII G'CGCG_C
Cuts at: 661 661
Size: 3968
BssSI C'ACGA_G
Cuts at: 532 2239 2597 532
Size: 1707 358 1903
Fragments arranged by size:
1903 1707 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1345 1345
Size: 3968
BstBI TT'CG_AA
Cuts at: 2329 2329
Size: 3968
BstYI r'GATC_y
Cuts at: 1 993 1460 1483 1818 2064 3076 3162 3174
Size: 992 467 23 335 246 1012 86 12
Cuts at: 3174 3520 1
Size: 346 449
Fragments arranged by size:
1012 992 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 128 258 107
Size: 21 130 3817
Fragments arranged by size:
3817 130 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1918 2099 2262 1918
Size: 181 163 3624
Fragments arranged by size:
3624 181 163
BtsI GCAGTG_nn'
Cuts at: 1211 1211
Size: 3968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 225 260 225
Size: 35 3933
DraI TTT'AAA
Cuts at: 967 967
Size: 3968
DraIII CAC_nnn'GTG
Cuts at: 264 264
Size: 3968
DrdI GACnn_nn'nnGTC
Cuts at: 350 1674 2532 350
Size: 1324 858 1786
Fragments arranged by size:
1786 1324 858
EaeI y'GGCC_r
Cuts at: 598 710 908 1553 1727 2118 2145 3490 598
Size: 112 198 645 174 391 27 1345 1076
Fragments arranged by size:
1345 1076 645 391 198 174 112 27
EagI C'GGCC_G
Cuts at: 710 1553 710
Size: 843 3125
EarI CTCTTCn'nnn_
Cuts at: 1991 2201 1991
Size: 210 3758
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 511 2176 2486 2632 3778 511
Size: 1665 310 146 1146 701
Fragments arranged by size:
1665 1146 701 310 146
EcoICRI GAG'CTC
Cuts at: 980 980
Size: 3968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 1793 2225 2972 3240 255
Size: 1538 432 747 268 983
Fragments arranged by size:
1538 983 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1107 1107
Size: 3968
EcoRI G'AATT_C
Cuts at: 972 972
Size: 3968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1987 1987
Size: 3968
FspI TGC'GCA
Cuts at: 550 776 1749 550
Size: 226 973 2769
Fragments arranged by size:
2769 973 226
HaeII r_GCGC'y
Cuts at: 162 758 1402 1650 2672 3651 162
Size: 596 644 248 1022 979 479
Fragments arranged by size:
1022 979 644 596 479 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1810 1840 1842 1872 1810
Size: 30 2 30 3906
Fragments arranged by size:
3906 30 30 2
HincII GTy'rAC
Cuts at: 423 1007 423
Size: 584 3384
KasI G'GCGC_C
Cuts at: 1398 1646 1398
Size: 248 3720
KpnI G_GTAC'C
Cuts at: 988 988
Size: 3968
MluI A'CGCG_T
Cuts at: 240 3401 240
Size: 3161 807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 547 679 2639 2823 547
Size: 132 1960 184 1692
Fragments arranged by size:
1960 1692 184 132
MscI TGG'CCA
Cuts at: 910 1729 910
Size: 819 3149
MslI CAynn'nnrTG
Cuts at: 211 1957 2084 211
Size: 1746 127 2095
Fragments arranged by size:
2095 1746 127
MspA1I CmG'CkG
Cuts at: 285 505 772 1079 1393 1753 2766 3011 285
Size: 220 267 307 314 360 1013 245 1242
Fragments arranged by size:
1242 1013 360 314 307 267 245 220
NaeI GCC'GGC
Cuts at: 598 2149 598
Size: 1551 2417
NarI GG'CG_CC
Cuts at: 1399 1647 1399
Size: 248 3720
NcoI C'CATG_G
Cuts at: 107 107
Size: 3968
NgoMIV G'CCGG_C
Cuts at: 596 2147 596
Size: 1551 2417
NspI r_CATG'y
Cuts at: 720 1021 1521 2428 720
Size: 301 500 907 2260
Fragments arranged by size:
2260 907 500 301
PciI A'CATG_T
Cuts at: 2424 2424
Size: 3968
PflMI CCAn_nnn'nTGG
Cuts at: 621 621
Size: 3968
PmeI GTTT'AAAC
Cuts at: 967 967
Size: 3968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3139 3107
Size: 32 3936
PspOMI G'GGCC_C
Cuts at: 747 747
Size: 3968
PspXI vC'TCGA_Gb
Cuts at: 154 838 154
Size: 684 3284
PstI C_TGCA'G
Cuts at: 863 1015 1700 863
Size: 152 685 3131
Fragments arranged by size:
3131 685 152
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3968
PvuII CAG'CTG
Cuts at: 772 1393 1753 772
Size: 621 360 2987
Fragments arranged by size:
2987 621 360
RsrII CG'GwC_CG
Cuts at: 2163 2163
Size: 3968
SacI G_AGCT'C
Cuts at: 982 982
Size: 3968
SalI G'TCGA_C
Cuts at: 1005 1005
Size: 3968
SapI GCTCTTCn'nnn_
Cuts at: 1991 2201 1991
Size: 210 3758
SbfI CC_TGCA'GG
Cuts at: 1015 1015
Size: 3968
ScaI AGT'ACT
Cuts at: 3485 3485
Size: 3968
SfcI C'TryA_G
Cuts at: 33 609 859 1011 1696 2689 2880 33
Size: 576 250 152 685 993 191 1121
Fragments arranged by size:
1121 993 685 576 250 191 152
SfoI GGC'GCC
Cuts at: 1400 1648 1400
Size: 248 3720
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
SmaI CCC'GGG
Cuts at: 293 478 574 760 990 293
Size: 185 96 186 230 3271
Fragments arranged by size:
3271 230 186 185 96
SmlI C'TyrA_G
Cuts at: 154 838 940 1123 2038 2530 2792 154
Size: 684 102 183 915 492 262 1330
Fragments arranged by size:
1330 915 684 492 262 183 102
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3968
SphI G_CATG'C
Cuts at: 1021 1521 1021
Size: 500 3468
StyI C'CwwG_G
Cuts at: 107 1102 1456 107
Size: 995 354 2619
Fragments arranged by size:
2619 995 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 346 667 1551 1684 2143 2352 346
Size: 321 884 133 459 209 1962
Fragments arranged by size:
1962 884 459 321 209 133
TatI w'GTAC_w
Cuts at: 3483 3483
Size: 3968
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 3936
Tth111I GACn'n_nGTC
Cuts at: 1765 1765
Size: 3968
XbaI T'CTAG_A
Cuts at: 999 999
Size: 3968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 210 841 1463 210
Size: 631 622 2715
Fragments arranged by size:
2715 631 622
XhoI C'TCGA_G
Cuts at: 154 838 154
Size: 684 3284
XmaI C'CCGG_G
Cuts at: 291 476 572 758 988 291
Size: 185 96 186 230 3271
Fragments arranged by size:
3271 230 186 185 96
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I BsaXI
BsgI BsiWI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI