Restriction map for pF1KB6415
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AlwI        AocI        
ApoI        AscI        AseI        AsnI        Asp718I     
AspI        AvrII       BamHI       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BpmI        BpuAI       
BsaAI       BsaBI       BsaWI       BseAI       BseRI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrGI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstYI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HgaI        HincII      HindII      HindIII     HpaI        
HphI        KpnI        MaeI        MaeII       MamI        
MfeI        MflI        MluI        MroI        MseI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SapI        ScaI        
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XhoII       


###################################
# ONE CUT ENZYMES (   68 enzymes) #
###################################

AccI          424  GTCTAC               GT^[AC][GT]AC
AcyI          294  GGCGCC               G[GA]^CG[CT]C
AfaI          504  GTAC                 GT^AC
AhdI          192  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        566  GTGCAC               G^TGCAC
AlwNI         721  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       836  AGCGCT               AGC^GCT
ApaI           87  GGGCCC               GGGCC^C
ApaLI         566  GTGCAC               G^TGCAC
Asp700I       834  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         192  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         566  GTGCAC               G[AT]GC[AT]^C
AviII         758  TGCGCA               TGC^GCA
BalI          476  TGGCCA               TGG^CCA
BanII          87  GGGCCC               G[GA]GC[CT]^C
BbeI          294  GGCGCC               GGCGC^C
BlpI          237  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      237  GCTCAGC              GC^T[ACGT]AGC
BsaHI         294  GGCGCC               G[GA]^CG[CT]C
BsaI          636  GGTCTC               GGTCTC(1/5)
BsgI          103  CTGCAC               GTGCAG(16/14)
BsiHKAI       566  GTGCAC               G[AT]GC[AT]^C
BspMI         137  ACCTGC               ACCTGC(4/8)
BsrBI          26  GAGCGG               CCGCTC(-3/-3)
BsrDI         704  CATTGC               GCAATG(2/0)
BsrFI         120  GCCGGT               [GA]^CCGG[CT]
BsrI          212  ACTGG                ACTGG(1/-1)
BssHII        322  GCGCGC               G^CGCGC
BstXI         389  CCATTGTAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         237  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        120  GCCGGT               [GA]^CCGG[CT]
DpnII         382  GATC                 ^GATC
Eam1105I      192  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          406  GAAGAG               CTCTTC(1/4)
Eco47III      836  AGCGCT               AGC^GCT
FspI          758  TGCGCA               TGC^GCA
Hin1I         294  GGCGCC               G[GA]^CG[CT]C
KasI          294  GGCGCC               G^GCGCC
Ksp632I       406  GAAGAG               CTCTTC(1/4)
MboI          382  GATC                 ^GATC
MluNI         476  TGGCCA               TGG^CCA
MscI          476  TGGCCA               TGG^CCA
NarI          294  GGCGCC               GG^CGCC
NcoI          479  CCATGG               C^CATGG
NdeII         382  GATC                 ^GATC
NotI          172  GCGGCCGC             GC^GGCCGC
NspI          116  GCATGC               [GA]CATG^[CT]
PflMI         468  CCACTGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          139  CTGCAG               CTGCA^G
PvuII         556  CAGCTG               CAG^CTG
RsaI          504  GTAC                 GT^AC
Sau3AI        382  GATC                 ^GATC
SbfI          138  CCTGCAGG             CCTGCA^GG
SexAI         617  ACCTGGT              A^CC[AT]GGT
SfcI          139  CTGCAG               C^T[GA][CT]AG
SfoI          294  GGCGCC               GGC^GCC
SmaI          156  CCCGGG               CCC^GGG
SphI          116  GCATGC               GCATG^C
Sse8387I      138  CCTGCAGG             CCTGCA^GG
StuI          536  AGGCCT               AGG^CCT
TaqI          530  TCGA                 T^CGA
Tsp45I        511  GTGAC                ^GT[GC]AC
TthHB8I       530  TCGA                 T^CGA
Van91I        468  CCACTGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          590  CCATCATCCTCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         156  CCCGGG               C^CCGGG
XmaI          156  CCCGGG               C^CCGGG
XmnI          834  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AvaI          156  CCCGGG               C^[CT]CG[GA]G
              217  CCCGAG              
BglI           65  GCCGTCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              163  GCCCTGACGGC         
BsiEI         173  CGGCCG               CG[GA][CT]^CG
              179  CGGCCG              
BsmAI         691  GAGAC                GTCTC(1/5)
              637  GTCTC               
BsoBI         156  CCCGGG               C^[CT]CG[GA]G
              217  CCCGAG              
BssSI         598  CTCGTG               CACGAG(-5/-1)
              629  CTCGTG              
EagI          173  CGGCCG               C^GGCCG
              179  CGGCCG              
EclXI         173  CGGCCG               C^GGCCG
              179  CGGCCG              
Eco52I        173  CGGCCG               C^GGCCG
              179  CGGCCG              
EcoT14I       479  CCATGG               C^C[AT][AT]GG
              819  CCTTGG              
KspI           39  CCGCGG               CCGC^GG
              176  CCGCGG              
MaeIII        511  GTGAC                ^GT[ACGT]AC
              863  GTAAC               
MboII         406  GAAGA                GAAGA(8/7)
              329  TCTTC               
PpuMI         190  GGGACCC              [GA]G^G[AT]CC[CT]
              198  GGGTCCC             
SacII          39  CCGCGG               CCGC^GG
              176  CCGCGG              
StyI          479  CCATGG               C^C[AT][AT]GG
              819  CCTTGG              
TspRI         469  CACTG                CA[GC]TG[ACGT][ACGT]^
              509  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BanI        
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DraII       DsaI        EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NlaIII      NlaIV       PleI        Sau96I      ScrFI       
SfaNI       TseI        
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