Restriction map for pF1KB6415
 (Linear) (Six-base) MAPSORT of: KIBB6415.seq  Check: 6380  from: 1  to: 924



KIBB6415.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AccI GT'mk_AC

Cuts at:      0     425     924 
   Size:        425     499
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     924 
   Size:        374     550
 
AfeI AGC'GCT

Cuts at:      0     838     924 
   Size:        838      86
 
AhdI GACnn_n'nnGTC

Cuts at:      0     197     924 
   Size:        197     727
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     536     568     924 
   Size:        536      32     356

  Fragments arranged by size: 

                 536     356      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     726     924 
   Size:        726     198
 
ApaI G_GGCC'C

Cuts at:      0      91     924 
   Size:         91     833
 
ApaLI G'TGCA_C

Cuts at:      0     566     924 
   Size:        566     358
 
AvaI C'yCGr_G

Cuts at:      0     156     217     924 
   Size:        156      61     707

  Fragments arranged by size: 

                 707     156      61
 
BanI G'GyrC_C

Cuts at:      0     160     294     799     924 
   Size:        160     134     505     125

  Fragments arranged by size: 

                 505     160     134     125
 
BanII G_rGCy'C

Cuts at:      0      91     924 
   Size:         91     833
 
BbeI G_GCGC'C

Cuts at:      0     298     924 
   Size:        298     626
 
BglI GCCn_nnn'nGGC

Cuts at:      0      71     169     924 
   Size:         71      98     755

  Fragments arranged by size: 

                 755      98      71
 
BlpI GC'TnA_GC

Cuts at:      0     238     924 
   Size:        238     686
 
Bme1580I G_kGCm'C

Cuts at:      0      91     165     374     570     590     924 
   Size:         91      74     209     196      20     334

  Fragments arranged by size: 

                 334     209     196      91      74      20
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     555     924 
   Size:        555     369
 
Bpu10I CC'TnA_GC

Cuts at:      0     243     924 
   Size:        243     681
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     642     924 
   Size:        642     282
 
BsaHI Gr'CG_yC

Cuts at:      0     295     924 
   Size:        295     629
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      27      57     924 
   Size:         27      30     867

  Fragments arranged by size: 

                 867      30      27
 
BseYI C'CCAG_C

Cuts at:      0     288     924 
   Size:        288     636
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     924 
   Size:         88     836
 
BsiEI CG_ry'CG

Cuts at:      0     176     182     924 
   Size:        176       6     742

  Fragments arranged by size: 

                 742     176       6
 
BsiHKAI G_wGCw'C

Cuts at:      0     570     924 
   Size:        570     354
 
Bsp1286I G_dGCh'C

Cuts at:      0      91     165     374     570     590     924 
   Size:         91      74     209     196      20     334

  Fragments arranged by size: 

                 334     209     196      91      74      20
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     146     924 
   Size:        146     778
 
BsrBI CCG'CTC

Cuts at:      0      28     924 
   Size:         28     896
 
BsrDI GCAATG_nn'

Cuts at:      0     703     924 
   Size:        703     221
 
BsrFI r'CCGG_y

Cuts at:      0     120     924 
   Size:        120     804
 
BssHII G'CGCG_C

Cuts at:      0     322     924 
   Size:        322     602
 
BssSI C'ACGA_G

Cuts at:      0     598     629     924 
   Size:        598      31     295

  Fragments arranged by size: 

                 598     295      31
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     396     924 
   Size:        396     528
 
BtgI C'CryG_G

Cuts at:      0      39     176     479     924 
   Size:         39     137     303     445

  Fragments arranged by size: 

                 445     303     137      39
 
BtsI GCAGTG_nn'

Cuts at:      0     468     924 
   Size:        468     456
 
EaeI y'GGCC_r

Cuts at:      0     173     179     465     476     924 
   Size:        173       6     286      11     448

  Fragments arranged by size: 

                 448     286     173      11       6
 
EagI C'GGCC_G

Cuts at:      0     173     179     924 
   Size:        173       6     745

  Fragments arranged by size: 

                 745     173       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     401     924 
   Size:        401     523
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     924 
   Size:        374     550
 
EcoO109I rG'GnC_Cy

Cuts at:      0      87      88     191     199     924 
   Size:         87       1     103       8     725

  Fragments arranged by size: 

                 725     103      87       8       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     543     858     897     924 
   Size:        543     315      39      27

  Fragments arranged by size: 

                 543     315      39      27
 
FspI TGC'GCA

Cuts at:      0     760     924 
   Size:        760     164
 
HaeII r_GCGC'y

Cuts at:      0      66     151     298     317     727     840     924 
   Size:         66      85     147      19     410     113      84

  Fragments arranged by size: 

                 410     147     113      85      84      66      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     183     215     924 
   Size:        183      32     709

  Fragments arranged by size: 

                 709     183      32
 
KasI G'GCGC_C

Cuts at:      0     294     924 
   Size:        294     630
 
MscI TGG'CCA

Cuts at:      0     478     924 
   Size:        478     446
 
MslI CAynn'nnrTG

Cuts at:      0     121     377     394     478     598     713     924 
   Size:        121     256      17      84     120     115     211

  Fragments arranged by size: 

                 256     211     121     120     115      84      17
 
MspA1I CmG'CkG

Cuts at:      0      23      41     102     178     455     558     700     924 
   Size:         23      18      61      76     277     103     142     224

  Fragments arranged by size: 

                 277     224     142     103      76      61      23      18
            
 
NarI GG'CG_CC

Cuts at:      0     295     924 
   Size:        295     629
 
NcoI C'CATG_G

Cuts at:      0     479     924 
   Size:        479     445
 
NotI GC'GGCC_GC

Cuts at:      0     173     924 
   Size:        173     751
 
NspI r_CATG'y

Cuts at:      0     120     924 
   Size:        120     804
 
PasI CC'CwG_GG

Cuts at:      0      83     195     924 
   Size:         83     112     729

  Fragments arranged by size: 

                 729     112      83
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     474     924 
   Size:        474     450
 
PpuMI rG'GwC_Cy

Cuts at:      0     191     199     924 
   Size:        191       8     725

  Fragments arranged by size: 

                 725     191       8
 
PspOMI G'GGCC_C

Cuts at:      0      87     924 
   Size:         87     837
 
PstI C_TGCA'G

Cuts at:      0     143     924 
   Size:        143     781
 
PvuII CAG'CTG

Cuts at:      0     558     924 
   Size:        558     366
 
SacII CC_GC'GG

Cuts at:      0      42     179     924 
   Size:         42     137     745

  Fragments arranged by size: 

                 745     137      42
 
SanDI GG'GwC_CC

Cuts at:      0     191     199     924 
   Size:        191       8     725

  Fragments arranged by size: 

                 725     191       8
 
SbfI CC_TGCA'GG

Cuts at:      0     143     924 
   Size:        143     781
 
SexAI A'CCwGG_T

Cuts at:      0     617     924 
   Size:        617     307
 
SfcI C'TryA_G

Cuts at:      0     139     924 
   Size:        139     785
 
SfoI GGC'GCC

Cuts at:      0     296     924 
   Size:        296     628
 
SmaI CCC'GGG

Cuts at:      0     158     924 
   Size:        158     766
 
SphI G_CATG'C

Cuts at:      0     120     924 
   Size:        120     804
 
StuI AGG'CCT

Cuts at:      0     538     924 
   Size:        538     386
 
StyI C'CwwG_G

Cuts at:      0     479     819     924 
   Size:        479     340     105

  Fragments arranged by size: 

                 479     340     105
 
TatI w'GTAC_w

Cuts at:      0     503     924 
   Size:        503     421
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27      59     924 
   Size:         27      32     865

  Fragments arranged by size: 

                 865      32      27
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     597     924 
   Size:        597     327
 
XmaI C'CCGG_G

Cuts at:      0     156     924 
   Size:        156     768
 
XmnI GAAnn'nnTTC

Cuts at:      0     838     924 
   Size:        838      86
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AhdI     AloI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII     BbeI     BglI     BlpI Bme1580I     BplI
   Bpu10I     BsaI    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstXI
     BtgI     BtsI     EaeI     EagI     EarI  Eco57MI EcoO109I     FalI
     FspI    HaeII    Hin4I     KasI     MscI     MslI   MspA1I     NarI
     NcoI     NotI     NspI     PasI    PflMI    PpuMI   PspOMI     PstI
    PvuII    SacII    SanDI     SbfI    SexAI     SfcI     SfoI     SmaI
     SphI     StuI     StyI     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AleI
     AlfI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmrI     BmtI     BpmI    BpuEI    BsaAI    BsaBI    BsaWI    BseRI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrGI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     NaeI     NdeI   NgoMIV     NheI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI     SalI     SapI     ScaI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII
  Tth111I     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com