Restriction map for pF1KE0363
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BglII       BlnI        BsaAI       
BsaHI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BsrBI       BsrGI       BssHII      BssSI       BstBI       
BstEII      BstPI       BstXI       ClaI        CpoI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoT14I     
FauI        FseI        FspI        HaeII       HgaI        
Hin1I       HpaI        KasI        KpnI        KspI        
MaeII       MluI        MluNI       MroI        MscI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         2013  GTATAC               GT^[AC][GT]AC
AccII          66  CGCG                 CG^CG
AflIII       2100  ACATGT               A^C[GA][CT]GT
AgeI          280  ACCGGT               A^CCGGT
AlwNI         203  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
BanII          25  GAGCCC               G[GA]GC[CT]^C
BclI         2073  TGATCA               T^GATCA
BcnI         1117  CCCGG                CC^[GC]GG
BlpI         2189  GCTTAGC              GC^T[ACGT]AGC
BmyI           25  GAGCCC               G[AGT]GC[ACT]^C
BpmI         1815  CTGGAG               CTGGAG(16/14)
Bpu1102I     2189  GCTTAGC              GC^T[ACGT]AGC
BsaBI         944  GATTGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         280  ACCGGT               [AT]^CCGG[AT]
BsmI          872  GAATGC               GAATGC(1/-1)
Bsp1286I       25  GAGCCC               G[AGT]GC[ACT]^C
BspHI         138  TCATGA               T^CATGA
BspLU11I     2100  ACATGT               A^CATGT
BspMI        1044  ACCTGC               ACCTGC(4/8)
BsrFI         280  ACCGGT               [GA]^CCGG[CT]
Bst1107I     2013  GTATAC               GTA^TAC
BstNI          29  CCTGG                CC^[AT]GG
BstUI          66  CGCG                 CG^CG
BstYI         204  AGATCC               [GA]^GATC[CT]
BstZ17I      2013  GTATAC               GTA^TAC
CelII        2189  GCTTAGC              GC^T[ACGT]AGC
Cfr10I        280  ACCGGT               [GA]^CCGG[CT]
DraI          333  TTTAAA               TTT^AAA
EcoRII         29  CCTGG                ^CC[AT]GG
EcoRV        1810  GATATC               GAT^ATC
EcoT22I      1326  ATGCAT               ATGCA^T
FbaI         2073  TGATCA               T^GATCA
HincII       1591  GTTGAC               GT[CT]^[GA]AC
HindII       1591  GTTGAC               GT[CT]^[GA]AC
HindIII      2187  AAGCTT               A^AGCTT
HphI         1267  GGTGA                GGTGA(8/7)
MamI          944  GATTGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1232  CAATTG               C^AATTG
MflI          204  AGATCC               [GA]^GATC[CT]
MslI          843  CATGAATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1232  CAATTG               C^AATTG
MvaI           29  CCTGG                CC^[AT]GG
MvnI           66  CGCG                 CG^CG
NciI         1117  CCCGG                CC^[GC]GG
NsiI         1326  ATGCAT               ATGCA^T
PinAI         280  ACCGGT               A^CCGGT
RcaI          138  TCATGA               T^CATGA
SapI         1149  GCTCTTC              GCTCTTC(1/4)
SfcI         2174  CTGTAG               C^T[GA][CT]AG
SmlI          226  CTCAAG               C^T[CT][GA]AG
Tsp45I       2097  GTCAC                ^GT[GC]AC
XhoII         204  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AocI           16  CCTCAGG              CC^T[ACGT]AGG
             1771  CCTGAGG             
BbsI          276  GAAGAC               GAAGAC(2/6)
              905  GTCTTC              
BpuAI         276  GAAGAC               GAAGAC(2/6)
              905  GTCTTC              
BsaJI          28  CCCTGG               C^C[ACGT][ACGT]GG
               29  CCTGGG              
BsmFI          32  GGGAC                GGGAC(10/14)
             1946  GGGAC               
BssKI          29  CCTGG                ^CC[ACGT]GG
             1117  CCCGG               
Bsu36I         16  CCTCAGG              CC^T[ACGT]AGG
             1771  CCTGAGG             
CfoI           65  GCGC                 GCG^C
             1416  GCGC                
Eco81I         16  CCTCAGG              CC^T[ACGT]AGG
             1771  CCTGAGG             
HapII         281  CCGG                 C^CGG
             1118  CCGG                
HhaI           65  GCGC                 GCG^C
             1416  GCGC                
HinP1I         65  GCGC                 G^CGC
             1416  GCGC                
HpaII         281  CCGG                 C^CGG
             1118  CCGG                
MspI          281  CCGG                 C^CGG
             1118  CCGG                
NspI         1332  ACATGC               [GA]CATG^[CT]
             2100  ACATGT              
ScrFI          29  CCTGG                CC^[ACGT]GG
             1117  CCCGG               
TaqI          342  TCGA                 T^CGA
             1716  TCGA                
TthHB8I       342  TCGA                 T^CGA
             1716  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbvI        BfaI        BsaI        
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsrDI       BsrI        BstAPI      BstF5I      Cac8I       
Cfr13I      DdeI        DpnII       EarI        Fnu4HI      
FokI        HaeIII      HinfI       ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MspA1I      MwoI        NdeI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      SfaNI       SspI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       
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