Restriction map for pF1KE0205
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvrII       BamHI       
BanII       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaI        
BseAI       BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DdeI        DraI        DraII       
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HpaI        KpnI        
Ksp632I     KspI        MaeI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tsp45I      TspRI       Tth111I     TthHB8I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AcsI          538  GAATTT               [GA]^AATT[CT]
AgeI           26  ACCGGT               A^CCGGT
AlwI           90  GATCC                GGATC(4/5)
ApoI          538  GAATTT               [GA]^AATT[CT]
AvaI          380  CCCGGG               C^[CT]CG[GA]G
AvaII         384  GGTCC                G^G[AT]CC
AviII         455  TGCGCA               TGC^GCA
BbeI            5  GGCGCC               GGCGC^C
BbvI          174  GCAGC                GCAGC(8/12)
BglI          190  GCCCTGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI          26  ACCGGT               [AT]^CCGG[AT]
BseRI         433  GAGGAG               GAGGAG(10/8)
BsgI          634  CTGCAC               GTGCAG(16/14)
BsoBI         380  CCCGGG               C^[CT]CG[GA]G
BsrFI          26  ACCGGT               [GA]^CCGG[CT]
BsrI          602  CCAGT                ACTGG(1/-1)
BstYI          89  AGATCC               [GA]^GATC[CT]
Cac8I          16  GCTCGC               GC[ACGT]^[ACGT]GC
Cfr10I         26  ACCGGT               [GA]^CCGG[CT]
DpnII          90  GATC                 ^GATC
EcoNI         452  CCTTGCGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       614  CCTTGG               C^C[AT][AT]GG
FauI          627  GCGGG                CCCGC(4/6)
FspI          455  TGCGCA               TGC^GCA
HaeII           5  GGCGCC               [GA]GCGC^[CT]
HgaI          482  GACGC                GACGC(5/10)
HindIII       421  AAGCTT               A^AGCTT
HphI          228  GGTGA                GGTGA(8/7)
KasI            5  GGCGCC               G^GCGCC
MaeII         596  ACGT                 A^CGT
MaeIII         23  GTTAC                ^GT[ACGT]AC
MboI           90  GATC                 ^GATC
MflI           89  AGATCC               [GA]^GATC[CT]
MseI          527  TTAA                 T^TAA
MslI          258  CATGCGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        175  CAGCTG               C[AC]G^C[GT]G
NarI            5  GGCGCC               GG^CGCC
NdeII          90  GATC                 ^GATC
PinAI          26  ACCGGT               A^CCGGT
PleI          587  GAGTC                GAGTC(4/5)
PvuII         175  CAGCTG               CAG^CTG
Sau3AI         90  GATC                 ^GATC
SfcI          374  CTGTAG               C^T[GA][CT]AG
SfoI            5  GGCGCC               GGC^GCC
SmaI          380  CCCGGG               CCC^GGG
StyI          614  CCTTGG               C^C[AT][AT]GG
TfiI          492  GATTC                G^A[AT]TC
Tru9I         527  TTAA                 T^TAA
TseI          174  GCAGC                G^C[AT]GC
Tsp509I       539  AATT                 ^AATT
XcmI          215  CCAACAAATACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          89  AGATCC               [GA]^GATC[CT]
XmaCI         380  CCCGGG               C^CCGGG
XmaI          380  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI            5  GGCGCC               G[GA]^CG[CT]C
              482  GACGCC              
BalI          147  TGGCCA               TGG^CCA
              554  TGGCCA              
BanI            5  GGCGCC               G^G[CT][GA]CC
              349  GGTGCC              
BbsI          544  GAAGAC               GAAGAC(2/6)
              180  GTCTTC              
BcnI          380  CCCGG                CC^[GC]GG
              381  CCGGG               
BpmI          331  CTCCAG               CTGGAG(16/14)
              193  CTGGAG              
BpuAI         544  GAAGAC               GAAGAC(2/6)
              180  GTCTTC              
BsaBI          90  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              492  GATTCATATC          
BsaHI           5  GGCGCC               G[GA]^CG[CT]C
              482  GACGCC              
CfoI            6  GCGC                 GCG^C
              456  GCGC                
DraIII        140  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              151  CACATGGTG           
EaeI          147  TGGCCA               [CT]^GGCC[GA]
              554  TGGCCA              
HapII          27  CCGG                 C^CGG
              381  CCGG                
HhaI            6  GCGC                 GCG^C
              456  GCGC                
Hin1I           5  GGCGCC               G[GA]^CG[CT]C
              482  GACGCC              
HinP1I          6  GCGC                 G^CGC
              456  GCGC                
HinfI         492  GATTC                G^A[ACGT]TC
              587  GAGTC               
HpaII          27  CCGG                 C^CGG
              381  CCGG                
MamI           90  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              492  GATTCATATC          
MluNI         147  TGGCCA               TGG^CCA
              554  TGGCCA              
MscI          147  TGGCCA               TGG^CCA
              554  TGGCCA              
MspI           27  CCGG                 C^CGG
              381  CCGG                
NciI          380  CCCGG                CC^[GC]GG
              381  CCGGG               
NspI          257  ACATGC               [GA]CATG^[CT]
              438  GCATGT              
PflMI         139  CCACTCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              503  CCAAGAAGTGG         
Van91I        139  CCACTCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              503  CCAAGAAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AciI        AfaI        AluI        BsaJI       BsiYI       
BslI        BssKI       BstF5I      BstNI       Cfr13I      
EcoRII      Fnu4HI      FokI        HaeIII      ItaI        
MboII       MnlI        MvaI        MwoI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       SfaNI       
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