Restriction map for pF1KB6392
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MroI        MslI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XcmI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        282  GGTACC               G^GTACC
AlwNI         109  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       282  GGTACC               G^GTACC
AspHI         529  GAGCAC               G[AT]GC[AT]^C
AvaI          399  CTCGAG               C^[CT]CG[GA]G
BalI          732  TGGCCA               TGG^CCA
BanI          282  GGTACC               G^G[CT][GA]CC
BcnI          588  CCGGG                CC^[GC]GG
BlpI            5  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I        5  GCTCAGC              GC^T[ACGT]AGC
BsiHKAI       529  GAGCAC               G[AT]GC[AT]^C
BsoBI         399  CTCGAG               C^[CT]CG[GA]G
BssSI         459  CTCGTG               CACGAG(-5/-1)
CelII           5  GCTCAGC              GC^T[ACGT]AGC
CfoI          617  GCGC                 GCG^C
DraII         250  GGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          246  CCACGG               C^C[GA][CT]GG
EaeI          732  TGGCCA               [CT]^GGCC[GA]
EcoO109I      250  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       806  CCTTGG               C^C[AT][AT]GG
HhaI          617  GCGC                 GCG^C
HinP1I        617  GCGC                 G^CGC
HinfI         780  GATTC                G^A[ACGT]TC
HphI          817  TCACC                GGTGA(8/7)
KpnI          282  GGTACC               GGTAC^C
MboII         882  GAAGA                GAAGA(8/7)
MluNI         732  TGGCCA               TGG^CCA
MscI          732  TGGCCA               TGG^CCA
NciI          588  CCGGG                CC^[GC]GG
PaeR7I        399  CTCGAG               C^TCGAG
PflMI         669  CCAGAACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         250  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          256  CTGCAG               CTGCA^G
SbfI          255  CCTGCAGG             CCTGCA^GG
SphI          480  GCATGC               GCATG^C
Sse8387I      255  CCTGCAGG             CCTGCA^GG
StyI          806  CCTTGG               C^C[AT][AT]GG
TfiI          780  GATTC                G^A[AT]TC
Tsp45I        404  GTGAC                ^GT[GC]AC
Van91I        669  CCAGAACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          399  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          319  AAATTT               [GA]^AATT[CT]
              451  AAATTC              
ApoI          319  AAATTT               [GA]^AATT[CT]
              451  AAATTC              
BanII           3  GGGCTC               G[GA]GC[CT]^C
              536  GAGCCC              
BbvI          183  GCAGC                GCAGC(8/12)
              614  GCTGC               
BsmFI         250  GGGAC                GGGAC(10/14)
              547  GGGAC               
BsmI          139  GAATGC               GAATGC(1/-1)
              430  GAATGC              
BsrDI         801  CATTGC               GCAATG(2/0)
              605  GCAATG              
Fnu4HI        183  GCAGC                GC^[ACGT]GC
              614  GCTGC               
HapII          70  CCGG                 C^CGG
              588  CCGG                
HpaII          70  CCGG                 C^CGG
              588  CCGG                
ItaI          183  GCAGC                GC^[ACGT]GC
              614  GCTGC               
MseI          316  TTAA                 T^TAA
              569  TTAA                
MspA1I          8  CAGCTG               C[AC]G^C[GT]G
              184  CAGCTG              
MspI           70  CCGG                 C^CGG
              588  CCGG                
NspI          179  ACATGC               [GA]CATG^[CT]
              480  GCATGC              
PvuII           8  CAGCTG               CAG^CTG
              184  CAGCTG              
SfcI          256  CTGCAG               C^T[GA][CT]AG
              495  CTGTAG              
SmlI          399  CTCGAG               C^T[CT][GA]AG
              738  CTCAAG              
TaqI          400  TCGA                 T^CGA
              599  TCGA                
Tru9I         316  TTAA                 T^TAA
              569  TTAA                
TseI          183  GCAGC                G^C[AT]GC
              614  GCTGC               
Tsp509I       320  AATT                 ^AATT
              452  AATT                
TthHB8I       400  TCGA                 T^CGA
              599  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AfaI        AluI        AvaII       BmyI        BpmI        
BsaI        BsaJI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstAPI      BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        EcoRII      
FokI        HaeIII      MaeIII      MnlI        MvaI        
MwoI        NlaIII      NlaIV       RsaI        Sau96I      
ScrFI       SfaNI       TspRI       
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