Restriction map for pF1KB6392
 (Linear) (Six-base) MAPSORT of: KIBB6392.seq  Check: 6694  from: 1  to: 909



KIBB6392.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

Acc65I G'GTAC_C

Cuts at:      0     282     909 
   Size:        282     627
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     345     377     909 
   Size:        345      32     532

  Fragments arranged by size: 

                 532     345      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     114     909 
   Size:        114     795
 
ApoI r'AATT_y

Cuts at:      0     319     451     909 
   Size:        319     132     458

  Fragments arranged by size: 

                 458     319     132
 
AvaI C'yCGr_G

Cuts at:      0     399     909 
   Size:        399     510
 
BanI G'GyrC_C

Cuts at:      0     282     909 
   Size:        282     627
 
BanII G_rGCy'C

Cuts at:      0       7     540     909 
   Size:          7     533     369

  Fragments arranged by size: 

                 533     369       7
 
BlpI GC'TnA_GC

Cuts at:      0       6     909 
   Size:          6     903
 
Bme1580I G_kGCm'C

Cuts at:      0     391     580     909 
   Size:        391     189     329

  Fragments arranged by size: 

                 391     329     189
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     574     909 
   Size:        574     335
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     118     652     909 
   Size:         77      41     534     257

  Fragments arranged by size: 

                 534     257      77      41
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     723     909 
   Size:        723     186
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      53     215     495     909 
   Size:         53     162     280     414

  Fragments arranged by size: 

                 414     280     162      53
 
BsiHKAI G_wGCw'C

Cuts at:      0     533     909 
   Size:        533     376
 
BsmI GAATG_Cn'

Cuts at:      0     145     436     909 
   Size:        145     291     473

  Fragments arranged by size: 

                 473     291     145
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     391     533     540     580     909 
   Size:          7     384     142       7      40     329

  Fragments arranged by size: 

                 384     329     142      40       7       7
 
BsrDI GCAATG_nn'

Cuts at:      0     612     800     909 
   Size:        612     188     109

  Fragments arranged by size: 

                 612     188     109
 
BssSI C'ACGA_G

Cuts at:      0     459     909 
   Size:        459     450
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      95     155     164     638     909 
   Size:         95      60       9     474     271

  Fragments arranged by size: 

                 474     271      95      60       9
 
BtgI C'CryG_G

Cuts at:      0     246     909 
   Size:        246     663
 
BtsI GCAGTG_nn'

Cuts at:      0     580     909 
   Size:        580     329
 
EaeI y'GGCC_r

Cuts at:      0     732     909 
   Size:        732     177
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     118     652     909 
   Size:         77      41     534     257

  Fragments arranged by size: 

                 534     257      77      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     251     909 
   Size:        251     658
 
KpnI G_GTAC'C

Cuts at:      0     286     909 
   Size:        286     623
 
MscI TGG'CCA

Cuts at:      0     734     909 
   Size:        734     175
 
MspA1I CmG'CkG

Cuts at:      0      10     186     909 
   Size:         10     176     723

  Fragments arranged by size: 

                 723     176      10
 
NspI r_CATG'y

Cuts at:      0     183     484     909 
   Size:        183     301     425

  Fragments arranged by size: 

                 425     301     183
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     675     909 
   Size:        675     234
 
PfoI T'CCnGG_A

Cuts at:      0      54     134     909 
   Size:         54      80     775

  Fragments arranged by size: 

                 775      80      54
 
PpuMI rG'GwC_Cy

Cuts at:      0     251     909 
   Size:        251     658
 
PspXI vC'TCGA_Gb

Cuts at:      0     399     909 
   Size:        399     510
 
PstI C_TGCA'G

Cuts at:      0     260     909 
   Size:        260     649
 
PvuII CAG'CTG

Cuts at:      0      10     186     909 
   Size:         10     176     723

  Fragments arranged by size: 

                 723     176      10
 
SanDI GG'GwC_CC

Cuts at:      0     251     909 
   Size:        251     658
 
SbfI CC_TGCA'GG

Cuts at:      0     260     909 
   Size:        260     649
 
SfcI C'TryA_G

Cuts at:      0     256     495     909 
   Size:        256     239     414

  Fragments arranged by size: 

                 414     256     239
 
SmlI C'TyrA_G

Cuts at:      0     399     738     909 
   Size:        399     339     171

  Fragments arranged by size: 

                 399     339     171
 
SphI G_CATG'C

Cuts at:      0     484     909 
   Size:        484     425
 
StyI C'CwwG_G

Cuts at:      0     806     909 
   Size:        806     103
 
TatI w'GTAC_w

Cuts at:      0     176     909 
   Size:        176     733
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     130     162     404     436     883     909 
   Size:        130      32     242      32     447      26

  Fragments arranged by size: 

                 447     242     130      32      32      26
 
XhoI C'TCGA_G

Cuts at:      0     399     909 
   Size:        399     510
 

 Enzymes that do cut:

   Acc65I     AloI    AlwNI     ApoI     AvaI     BanI    BanII     BlpI
 Bme1580I     BmrI     BpmI    BpuEI     BsaI  BsiHKAI     BsmI Bsp1286I
    BsrDI    BssSI   BstAPI     BtgI     BtsI     EaeI  Eco57MI EcoO109I
     KpnI     MscI   MspA1I     NspI    PflMI     PfoI    PpuMI    PspXI
     PstI    PvuII    SanDI     SbfI     SfcI     SmlI     SphI     StyI
     TatI     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI     BplI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SapI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XmaI     XmnI
     ZraI
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