Restriction map for pF1KB8237
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspHI       AspI        AviII       AvrII       
BamHI       BanII       BbeI        BbvCI       BcgI        
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MflI        
MluI        MroI        MseI        MunI        NarI        
NcoI        NdeI        NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        Tru9I       Tsp509I     Tth111I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   58 enzymes) #
###################################

AcyI          570  GACGCC               G[GA]^CG[CT]C
AfaI          582  GTAC                 GT^AC
AhdI          265  GACTCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         104  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         265  GACTCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          629  CCCGAG               C^[CT]CG[GA]G
BalI          485  TGGCCA               TGG^CCA
BbrPI         601  CACGTG               CAC^GTG
BciVI         697  GGATAC               GTATCC(6/5)
BcnI           79  CCCGG                CC^[GC]GG
BfaI          262  CTAG                 C^TAG
BglI          619  GCCTTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          760  GTGCCC               G[AGT]GC[ACT]^C
BsaAI         601  CACGTG               [CT]AC^GT[GA]
BsaHI         570  GACGCC               G[GA]^CG[CT]C
BsaI          409  GAGACC               GGTCTC(1/5)
BseRI          85  GAGGAG               GAGGAG(10/8)
BsgI          132  CTGCAC               GTGCAG(16/14)
BsoBI         629  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      760  GTGCCC               G[AGT]GC[ACT]^C
BsrI          167  CCAGT                ACTGG(1/-1)
DpnII         720  GATC                 ^GATC
DraII          65  GGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          686  CCGTGG               C^C[GA][CT]GG
Eam1105I      265  GACTCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          742  CTCTTC               CTCTTC(1/4)
EcoO109I       65  GGGACCC              [GA]G^G[ACGT]CC[CT]
HgaI          570  GACGC                GACGC(5/10)
Hin1I         570  GACGCC               G[GA]^CG[CT]C
HincII        346  GTCAAC               GT[CT]^[GA]AC
HindII        346  GTCAAC               GT[CT]^[GA]AC
Ksp632I       742  CTCTTC               CTCTTC(1/4)
MaeI          262  CTAG                 C^TAG
MboI          720  GATC                 ^GATC
MluNI         485  TGGCCA               TGG^CCA
MscI          485  TGGCCA               TGG^CCA
MslI          669  CACCCCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          252  GCCGGC               GCC^GGC
NciI           79  CCCGG                CC^[GC]GG
NdeII         720  GATC                 ^GATC
NgoMIV        252  GCCGGC               G^CCGGC
PflMI         447  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         601  CACGTG               CAC^GTG
PmlI          601  CACGTG               CAC^GTG
PpuMI          65  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         310  CAGCTG               CAG^CTG
RsaI          582  GTAC                 GT^AC
SapI          741  GCTCTTC              GCTCTTC(1/4)
Sau3AI        720  GATC                 ^GATC
SexAI         755  ACCTGGT              A^CC[AT]GGT
SfaNI         163  GATGC                GCATC(5/9)
SfiI          618  GGCCTTGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          736  CTCAAG               C^T[CT][GA]AG
StuI          617  AGGCCT               AGG^CCT
TaqI          722  TCGA                 T^CGA
TfiI          150  GAATC                G^A[AT]TC
TthHB8I       722  TCGA                 T^CGA
Van91I        447  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         364  CGCG                 CG^CG
              592  CGCG                
BanI          436  GGTGCC               G^G[CT][GA]CC
              759  GGTGCC              
BbsI           22  GAAGAC               GAAGAC(2/6)
               73  GAAGAC              
BpmI          427  CTGGAG               CTGGAG(16/14)
              526  CTGGAG              
BpuAI          22  GAAGAC               GAAGAC(2/6)
               73  GAAGAC              
BsaWI         159  ACCGGA               [AT]^CCGG[AT]
              701  ACCGGA              
BsmAI         409  GAGAC                GTCTC(1/5)
              122  GTCTC               
BsmFI          65  GGGAC                GGGAC(10/14)
              568  GGGAC               
BsrFI         221  ACCGGC               [GA]^CCGG[CT]
              252  GCCGGC              
BstUI         364  CGCG                 CG^CG
              592  CGCG                
Cfr10I        221  ACCGGC               [GA]^CCGG[CT]
              252  GCCGGC              
DrdI          106  GACACTGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              337  GACAACCTCGTC        
EaeI          254  CGGCCA               [CT]^GGCC[GA]
              485  TGGCCA              
FauI          763  CCCGC                CCCGC(4/6)
              593  GCGGG               
HaeII         472  AGCGCC               [GA]GCGC^[CT]
              634  GGCGCT              
MaeII         536  ACGT                 A^CGT
              602  ACGT                
MaeIII        335  GTGAC                ^GT[ACGT]AC
              533  GTGAC               
MspA1I        310  CAGCTG               C[AC]G^C[GT]G
              764  CCGCTG              
MvnI          364  CGCG                 CG^CG
              592  CGCG                
PleI          265  GACTC                GAGTC(4/5)
              658  GACTC               
PstI           17  CTGCAG               CTGCA^G
               92  CTGCAG              
SfcI           17  CTGCAG               C^T[GA][CT]AG
               92  CTGCAG              
Tsp45I        335  GTGAC                ^GT[GC]AC
              533  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BsaJI       
BsiYI       BslI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MboII       MnlI        MspI        MvaI        MwoI        
NlaIV       Sau96I      ScrFI       TseI        TspRI       
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