Restriction map for pF1KB8237
 (Linear) (Six-base) MAPSORT of: KIBB8237.seq  Check: 4859  from: 1  to: 777



KIBB8237.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     217     334     777 
   Size:         92     125     117     443

  Fragments arranged by size: 

                 443     125     117      92
 
AhdI GACnn_n'nnGTC

Cuts at:      0     270     777 
   Size:        270     507
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      65     775     777 
   Size:         65     710       2

  Fragments arranged by size: 

                 710      65       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0     109     777 
   Size:        109     668
 
AvaI C'yCGr_G

Cuts at:      0     629     777 
   Size:        629     148
 
BanI G'GyrC_C

Cuts at:      0     436     759     777 
   Size:        436     323      18

  Fragments arranged by size: 

                 436     323      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      29      80     777 
   Size:         29      51     697

  Fragments arranged by size: 

                 697      51      29
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     691     777 
   Size:        691      86
 
BglI GCCn_nnn'nGGC

Cuts at:      0     625     777 
   Size:        625     152
 
Bme1580I G_kGCm'C

Cuts at:      0     764     777 
   Size:        764      13
 
BmgBI CAC'GTC

Cuts at:      0     537     777 
   Size:        537     240
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     547     777 
   Size:        448      99     230

  Fragments arranged by size: 

                 448     230      99
 
Bpu10I CC'TnA_GC

Cuts at:      0     140     777 
   Size:        140     637
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721     777 
   Size:        721      56
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     403     777 
   Size:        403     374
 
BsaAI yAC'GTr

Cuts at:      0     603     777 
   Size:        603     174
 
BsaHI Gr'CG_yC

Cuts at:      0     571     777 
   Size:        571     206
 
BsaWI w'CCGG_w

Cuts at:      0     159     701     777 
   Size:        159     542      76

  Fragments arranged by size: 

                 542     159      76
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     777 
   Size:        100     677
 
BseYI C'CCAG_C

Cuts at:      0      11      38     777 
   Size:         11      27     739

  Fragments arranged by size: 

                 739      27      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     777 
   Size:        117     660
 
Bsp1286I G_dGCh'C

Cuts at:      0     764     777 
   Size:        764      13
 
BsrFI r'CCGG_y

Cuts at:      0     221     252     777 
   Size:        221      31     525

  Fragments arranged by size: 

                 525     221      31
 
BtgI C'CryG_G

Cuts at:      0     686     777 
   Size:        686      91
 
BtsI GCAGTG_nn'

Cuts at:      0     574     777 
   Size:        574     203
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     112     343     777 
   Size:        112     231     434

  Fragments arranged by size: 

                 434     231     112
 
EaeI y'GGCC_r

Cuts at:      0     254     485     777 
   Size:        254     231     292

  Fragments arranged by size: 

                 292     254     231
 
EarI CTCTTCn'nnn_

Cuts at:      0     748     777 
   Size:        748      29
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     381     777 
   Size:        381     396
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     217     334     448     547     777 
   Size:         92     125     117     114      99     230

  Fragments arranged by size: 

                 230     125     117     114      99      92
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66     777 
   Size:         66     711
 
HaeII r_GCGC'y

Cuts at:      0     476     638     777 
   Size:        476     162     139

  Fragments arranged by size: 

                 476     162     139
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     777 
   Size:        256      32     489

  Fragments arranged by size: 

                 489     256      32
 
HincII GTy'rAC

Cuts at:      0     348     777 
   Size:        348     429
 
MscI TGG'CCA

Cuts at:      0     487     777 
   Size:        487     290
 
MslI CAynn'nnrTG

Cuts at:      0     673     777 
   Size:        673     104
 
MspA1I CmG'CkG

Cuts at:      0     312     766     777 
   Size:        312     454      11

  Fragments arranged by size: 

                 454     312      11
 
NaeI GCC'GGC

Cuts at:      0     254     777 
   Size:        254     523
 
NgoMIV G'CCGG_C

Cuts at:      0     252     777 
   Size:        252     525
 
PasI CC'CwG_GG

Cuts at:      0     693     777 
   Size:        693      84
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     453     777 
   Size:        453     324
 
PmlI CAC'GTG

Cuts at:      0     603     777 
   Size:        603     174
 
PpuMI rG'GwC_Cy

Cuts at:      0      66     777 
   Size:         66     711
 
PstI C_TGCA'G

Cuts at:      0      21      96     777 
   Size:         21      75     681

  Fragments arranged by size: 

                 681      75      21
 
PvuII CAG'CTG

Cuts at:      0     312     777 
   Size:        312     465
 
SanDI GG'GwC_CC

Cuts at:      0      66     777 
   Size:         66     711
 
SapI GCTCTTCn'nnn_

Cuts at:      0     748     777 
   Size:        748      29
 
SexAI A'CCwGG_T

Cuts at:      0     755     777 
   Size:        755      22
 
SfcI C'TryA_G

Cuts at:      0      17      92     777 
   Size:         17      75     685

  Fragments arranged by size: 

                 685      75      17
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     625     777 
   Size:        625     152
 
SmlI C'TyrA_G

Cuts at:      0     736     777 
   Size:        736      41
 
StuI AGG'CCT

Cuts at:      0     619     777 
   Size:        619     158
 

 Enzymes that do cut:

     AcuI     AhdI     AlfI    AlwNI     AvaI     BanI     BbsI    BciVI
     BglI Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BseRI    BseYI     BsgI Bsp1286I    BsrFI     BtgI
     BtsI     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII     MscI     MslI   MspA1I     NaeI   NgoMIV     PasI
    PflMI     PmlI    PpuMI     PstI    PvuII    SanDI     SapI    SexAI
     SfcI     SfiI     SmlI     StuI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
     BclI    BfrBI    BglII     BlpI     BmrI     BmtI     BplI    BsaBI
    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PfoI     PmeI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI     SbfI     ScaI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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