Restriction map for pF1KB6470
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        AspEI       AspHI       AspI        AvaII       
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

Acc65I        630  GGTACC               G^GTACC
AccII          27  CGCG                 CG^CG
AcyI          583  GACGCC               G[GA]^CG[CT]C
AflIII        749  ACGTGT               A^C[GA][CT]GT
AlwNI         691  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       747  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       630  GGTACC               G^GTACC
AvaI          269  CCCGGG               C^[CT]CG[GA]G
BalI         1055  TGGCCA               TGG^CCA
BanI          630  GGTACC               G^G[CT][GA]CC
BbvI           86  GCAGC                GCAGC(8/12)
BciVI         961  GGATAC               GTATCC(6/5)
BglI         1048  GCCCCGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         583  GACGCC               G[GA]^CG[CT]C
BsaWI         563  TCCGGT               [AT]^CCGG[AT]
BseRI         462  GAGGAG               GAGGAG(10/8)
BsgI          554  GTGCAG               GTGCAG(16/14)
BsmAI         680  GAGAC                GTCTC(1/5)
BsmFI         546  GTCCC                GGGAC(10/14)
BsoBI         269  CCCGGG               C^[CT]CG[GA]G
BspMI         704  ACCTGC               ACCTGC(4/8)
BsrDI         219  CATTGC               GCAATG(2/0)
BstAPI        254  GCATTCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        420  GGATG                GGATG(2/0)
BstUI          27  CGCG                 CG^CG
BstYI         590  AGATCC               [GA]^GATC[CT]
DdeI          720  CTCAG                C^T[ACGT]AG
DraIII        805  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          386  CTCTTC               CTCTTC(1/4)
EcoRV         880  GATATC               GAT^ATC
EcoT14I       470  CCATGG               C^C[AT][AT]GG
Fnu4HI         86  GCAGC                GC^[ACGT]GC
FokI          420  GGATG                GGATG(9/13)
HgaI          583  GACGC                GACGC(5/10)
Hin1I         583  GACGCC               G[GA]^CG[CT]C
ItaI           86  GCAGC                GC^[ACGT]GC
KpnI          630  GGTACC               GGTAC^C
Ksp632I       386  CTCTTC               CTCTTC(1/4)
MflI          590  AGATCC               [GA]^GATC[CT]
MluNI        1055  TGGCCA               TGG^CCA
MscI         1055  TGGCCA               TGG^CCA
MslI          966  CACTCACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        549  CCGCTG               C[AC]G^C[GT]G
MvnI           27  CGCG                 CG^CG
NaeI           91  GCCGGC               GCC^GGC
NcoI          470  CCATGG               C^CATGG
NgoMIV         91  GCCGGC               G^CCGGC
PleI           49  GACTC                GAGTC(4/5)
PstI          706  CTGCAG               CTGCA^G
SfcI          706  CTGCAG               C^T[GA][CT]AG
SfiI         1047  GGCCCCGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          269  CCCGGG               CCC^GGG
SmlI          459  CTTGAG               C^T[CT][GA]AG
StuI         1014  AGGCCT               AGG^CCT
StyI          470  CCATGG               C^C[AT][AT]GG
TfiI          840  GATTC                G^A[AT]TC
TseI           86  GCAGC                G^C[AT]GC
XhoII         590  AGATCC               [GA]^GATC[CT]
XmaCI         269  CCCGGG               C^CCGGG
XmaI          269  CCCGGG               C^CCGGG
XmnI          747  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          567  GTAC                 GT^AC
              631  GTAC                
AluI          137  AGCT                 AG^CT
              575  AGCT                
AlwI          591  GATCC                GGATC(4/5)
              798  GATCC               
BbsI          160  GAAGAC               GAAGAC(2/6)
             1038  GAAGAC              
BfaI          178  CTAG                 C^TAG
              652  CTAG                
BpmI          230  CTCCAG               CTGGAG(16/14)
              940  CTGGAG              
BpuAI         160  GAAGAC               GAAGAC(2/6)
             1038  GAAGAC              
BsmI          813  GAATGC               GAATGC(1/-1)
              254  GCATTC              
BsrFI          91  GCCGGC               [GA]^CCGG[CT]
              238  ACCGGC              
Cfr10I         91  GCCGGC               [GA]^CCGG[CT]
              238  ACCGGC              
DsaI          109  CCGTGG               C^C[GA][CT]GG
              470  CCATGG              
EaeI           93  CGGCCA               [CT]^GGCC[GA]
             1055  TGGCCA              
HaeII          88  AGCGCC               [GA]GCGC^[CT]
              693  GGCGCT              
HinfI          49  GACTC                G^A[ACGT]TC
              840  GATTC               
HphI          596  TCACC                GGTGA(8/7)
              871  TCACC               
MaeI          178  CTAG                 C^TAG
              652  CTAG                
MaeII         341  ACGT                 A^CGT
              749  ACGT                
MaeIII        382  GTCAC                ^GT[ACGT]AC
              764  GTGAC               
MseI          359  TTAA                 T^TAA
              737  TTAA                
RsaI          567  GTAC                 GT^AC
              631  GTAC                
SfaNI         298  GATGC                GCATC(5/9)
              858  GCATC               
Tru9I         359  TTAA                 T^TAA
              737  TTAA                
Tsp45I        382  GTCAC                ^GT[GC]AC
              764  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        ApaI        BanII       BcnI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstNI       Cac8I       CfoI        Cfr13I      
DpnII       DraII       EcoO109I    EcoRII      FauI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
MboI        MboII       MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       TaqI        Tsp509I     
TspRI       TthHB8I     
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