Restriction map for pF1KB6470
 (Linear) (Six-base) MAPSORT of: KIBB6470.seq  Check: 4400  from: 1  to: 1062



KIBB6470.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

Acc65I G'GTAC_C

Cuts at:      0     630    1062 
   Size:        630     432
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54    1062 
   Size:         54    1008
 
AflIII A'CryG_T

Cuts at:      0     749    1062 
   Size:        749     313
 
AlwNI CAG_nnn'CTG

Cuts at:      0     696    1062 
   Size:        696     366
 
ApaI G_GGCC'C

Cuts at:      0     229     276     286    1062 
   Size:        229      47      10     776

  Fragments arranged by size: 

                 776     229      47      10
 
AvaI C'yCGr_G

Cuts at:      0     269    1062 
   Size:        269     793
 
BanI G'GyrC_C

Cuts at:      0     630    1062 
   Size:        630     432
 
BanII G_rGCy'C

Cuts at:      0     229     276     286     450     539     773    1062 
   Size:        229      47      10     164      89     234     289

  Fragments arranged by size: 

                 289     234     229     164      89      47      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     167    1045    1062 
   Size:        167     878      17

  Fragments arranged by size: 

                 878     167      17
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     955    1062 
   Size:        955     107
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1054    1062 
   Size:       1054       8
 
Bme1580I G_kGCm'C

Cuts at:      0     209     229     276     286    1062 
   Size:        209      20      47      10     776

  Fragments arranged by size: 

                 776     209      47      20      10
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     961    1062 
   Size:        215     746     101

  Fragments arranged by size: 

                 746     215     101
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     480    1062 
   Size:        480     582
 
BsaHI Gr'CG_yC

Cuts at:      0     584    1062 
   Size:        584     478
 
BsaWI w'CCGG_w

Cuts at:      0     563    1062 
   Size:        563     499
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     477    1062 
   Size:        477     585
 
BseYI C'CCAG_C

Cuts at:      0     279     285     449    1062 
   Size:        279       6     164     613

  Fragments arranged by size: 

                 613     279     164       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     575    1062 
   Size:        575     487
 
BsmI GAATG_Cn'

Cuts at:      0     254     819    1062 
   Size:        254     565     243

  Fragments arranged by size: 

                 565     254     243
 
Bsp1286I G_dGCh'C

Cuts at:      0     209     229     276     286     450     539     773    1062 
   Size:        209      20      47      10     164      89     234     289

  Fragments arranged by size: 

                 289     234     209     164      89      47      20      10
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     713    1062 
   Size:        713     349
 
BsrDI GCAATG_nn'

Cuts at:      0     218    1062 
   Size:        218     844
 
BsrFI r'CCGG_y

Cuts at:      0      91     238    1062 
   Size:         91     147     824

  Fragments arranged by size: 

                 824     147      91
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     260    1062 
   Size:        260     802
 
BstYI r'GATC_y

Cuts at:      0     590    1062 
   Size:        590     472
 
BtgI C'CryG_G

Cuts at:      0     109     470    1062 
   Size:        109     361     592

  Fragments arranged by size: 

                 592     361     109
 
BtsI GCAGTG_nn'

Cuts at:      0     778    1062 
   Size:        778     284
 
DraIII CAC_nnn'GTG

Cuts at:      0     810    1062 
   Size:        810     252
 
EaeI y'GGCC_r

Cuts at:      0      93    1055    1062 
   Size:         93     962       7

  Fragments arranged by size: 

                 962      93       7
 
EarI CTCTTCn'nnn_

Cuts at:      0     392    1062 
   Size:        392     670
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     215     961    1062 
   Size:         54     161     746     101

  Fragments arranged by size: 

                 746     161     101      54
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78     226     273    1062 
   Size:         78     148      47     789

  Fragments arranged by size: 

                 789     148      78      47
 
EcoRV GAT'ATC

Cuts at:      0     882    1062 
   Size:        882     180
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     403    1062 
   Size:        403     659
 
HaeII r_GCGC'y

Cuts at:      0      92     697    1062 
   Size:         92     605     365

  Fragments arranged by size: 

                 605     365      92
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     392     424     831     863    1062 
   Size:        392      32     407      32     199

  Fragments arranged by size: 

                 407     392     199      32      32
 
KpnI G_GTAC'C

Cuts at:      0     634    1062 
   Size:        634     428
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     287    1062 
   Size:        287     775
 
MscI TGG'CCA

Cuts at:      0    1057    1062 
   Size:       1057       5
 
MslI CAynn'nnrTG

Cuts at:      0     970    1062 
   Size:        970      92
 
MspA1I CmG'CkG

Cuts at:      0     551    1062 
   Size:        551     511
 
NaeI GCC'GGC

Cuts at:      0      93    1062 
   Size:         93     969
 
NcoI C'CATG_G

Cuts at:      0     470    1062 
   Size:        470     592
 
NgoMIV G'CCGG_C

Cuts at:      0      91    1062 
   Size:         91     971
 
PasI CC'CwG_GG

Cuts at:      0     675    1062 
   Size:        675     387
 
PfoI T'CCnGG_A

Cuts at:      0     231    1062 
   Size:        231     831
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     736     768    1062 
   Size:        736      32     294

  Fragments arranged by size: 

                 736     294      32
 
PspOMI G'GGCC_C

Cuts at:      0     225     272     282    1062 
   Size:        225      47      10     780

  Fragments arranged by size: 

                 780     225      47      10
 
PstI C_TGCA'G

Cuts at:      0     710    1062 
   Size:        710     352
 
SfcI C'TryA_G

Cuts at:      0     706    1062 
   Size:        706     356
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1054    1062 
   Size:       1054       8
 
SmaI CCC'GGG

Cuts at:      0     271    1062 
   Size:        271     791
 
SmlI C'TyrA_G

Cuts at:      0     459    1062 
   Size:        459     603
 
SrfI GCCC'GGGC

Cuts at:      0     271    1062 
   Size:        271     791
 
StuI AGG'CCT

Cuts at:      0    1016    1062 
   Size:       1016      46
 
StyI C'CwwG_G

Cuts at:      0     470    1062 
   Size:        470     592
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     454     595     791    1062 
   Size:        454     141     196     271

  Fragments arranged by size: 

                 454     271     196     141
 
XmaI C'CCGG_G

Cuts at:      0     269    1062 
   Size:        269     793
 
XmnI GAAnn'nnTTC

Cuts at:      0     751    1062 
   Size:        751     311
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII    AlwNI     ApaI     AvaI     BanI    BanII
     BbsI    BciVI     BglI Bme1580I     BpmI    BpuEI    BsaHI    BsaWI
    BseRI    BseYI     BsgI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI
   BstAPI    BstYI     BtgI     BtsI   DraIII     EaeI     EarI  Eco57MI
 EcoO109I    EcoRV     FalI    HaeII    Hin4I     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     PasI     PfoI     PpiI
   PspOMI     PstI     SfcI     SfiI     SmaI     SmlI     SrfI     StuI
     StyI    TaqII     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     MfeI     MluI     NarI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI
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