Restriction map for pF1KB6355
 (Linear) (Six-base) MAPSORT of: KIBB6355.seq  Check: 9619  from: 1  to: 798



KIBB6355.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

ApoI r'AATT_y

Cuts at:      0     119     165     242     315     417     475     606     798 
   Size:        119      46      77      73     102      58     131     192

  Fragments arranged by size: 

                 192     131     119     102      77      73      58      46
            
 
BanI G'GyrC_C

Cuts at:      0     566     798 
   Size:        566     232
 
BanII G_rGCy'C

Cuts at:      0      74     798 
   Size:         74     724
 
BbeI G_GCGC'C

Cuts at:      0     570     798 
   Size:        570     228
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     542     719     798 
   Size:        542     177      79

  Fragments arranged by size: 

                 542     177      79
 
BclI T'GATC_A

Cuts at:      0     660     798 
   Size:        660     138
 
BglII A'GATC_T

Cuts at:      0     397     798 
   Size:        397     401
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     555     798 
   Size:        555     243
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     753     798 
   Size:        395     358      45

  Fragments arranged by size: 

                 395     358      45
 
BsaHI Gr'CG_yC

Cuts at:      0     567     798 
   Size:        567     231
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      82     798 
   Size:         82     716
 
BseYI C'CCAG_C

Cuts at:      0      97     798 
   Size:         97     701
 
BsiHKAI G_wGCw'C

Cuts at:      0      74     798 
   Size:         74     724
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     798 
   Size:         74     724
 
BspHI T'CATG_A

Cuts at:      0     723     798 
   Size:        723      75
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     335     798 
   Size:        335     463
 
BsrDI GCAATG_nn'

Cuts at:      0     203     798 
   Size:        203     595
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      97     798 
   Size:         97     701
 
BstYI r'GATC_y

Cuts at:      0     397     798 
   Size:        397     401
 
BtsI GCAGTG_nn'

Cuts at:      0     440     798 
   Size:        440     358
 
EarI CTCTTCn'nnn_

Cuts at:      0     411     750     798 
   Size:        411     339      48

  Fragments arranged by size: 

                 411     339      48
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      31     798 
   Size:         31     767
 
EcoICRI GAG'CTC

Cuts at:      0      72     798 
   Size:         72     726
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     753     798 
   Size:        395     358      45

  Fragments arranged by size: 

                 395     358      45
 
EcoRI G'AATT_C

Cuts at:      0     606     798 
   Size:        606     192
 
HaeII r_GCGC'y

Cuts at:      0     570     786     798 
   Size:        570     216      12

  Fragments arranged by size: 

                 570     216      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     652     684     798 
   Size:        652      32     114

  Fragments arranged by size: 

                 652     114      32
 
KasI G'GCGC_C

Cuts at:      0     566     798 
   Size:        566     232
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     798 
   Size:        291     507
 
MslI CAynn'nnrTG

Cuts at:      0     743     798 
   Size:        743      55
 
NarI GG'CG_CC

Cuts at:      0     567     798 
   Size:        567     231
 
NdeI CA'TA_TG

Cuts at:      0     465     798 
   Size:        465     333
 
PshAI GACnn'nnGTC

Cuts at:      0     459     798 
   Size:        459     339
 
SacI G_AGCT'C

Cuts at:      0      74     798 
   Size:         74     724
 
SfoI GGC'GCC

Cuts at:      0     568     798 
   Size:        568     230
 
StuI AGG'CCT

Cuts at:      0     234     798 
   Size:        234     564
 
StyI C'CwwG_G

Cuts at:      0     570     798 
   Size:        570     228
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      79     798 
   Size:         79     719
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     307     798 
   Size:        307     491
 
XmnI GAAnn'nnTTC

Cuts at:      0     419     606     798 
   Size:        419     187     192

  Fragments arranged by size: 

                 419     192     187
 

 Enzymes that do cut:

     ApoI     BanI    BanII     BbeI     BbsI     BclI    BglII     BmrI
     BpmI    BsaHI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrDI    BstXI    BstYI     BtsI     EarI     EciI  EcoICRI  Eco57MI
    EcoRI    HaeII    Hin4I     KasI     MmeI     MslI     NarI     NdeI
    PshAI     SacI     SfoI     StuI     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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