Restriction map for pF1KB5186
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        Alw44I      AlwNI       AocI        
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AspI        
AviII       AvrII       BamHI       BbrPI       BbsI        
BbvCI       BcgI        BfaI        BfrI        BglI        
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaWI       BseAI       BsiHKAI     
BsiWI       BsmFI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BssSI       
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraII       DraIII      DrdI        Eco81I      EcoNI       
EcoO109I    EcoRI       EcoRV       EcoT22I     FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        MaeI        MamI        MfeI        MluI        
MroI        MseI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SalI        SbfI        SexAI       
SfcI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TfiI        Tru9I       Tsp509I     TspRI       
Tth111I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          211  GTCTAC               GT^[AC][GT]AC
AflIII        233  ACACGT               A^C[GA][CT]GT
AhdI          367  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          360  GATCC                GGATC(4/5)
AspEI         367  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          581  TGGCCA               TGG^CCA
BanII         603  GAGCCC               G[GA]GC[CT]^C
BbeI          266  GGCGCC               GGCGC^C
BciVI          53  GTATCC               GTATCC(6/5)
BclI           17  TGATCA               T^GATCA
BglII         524  AGATCT               A^GATCT
BsaI          624  GGTCTC               GGTCTC(1/5)
BseRI         334  GAGGAG               GAGGAG(10/8)
BsiEI         138  CGGCCG               CG[GA][CT]^CG
BsmBI         287  GAGACG               CGTCTC(1/5)
Bsp1407I      779  TGTACA               T^GTACA
BspMI         611  ACCTGC               ACCTGC(4/8)
BsrGI         779  TGTACA               T^GTACA
BsrI            6  ACTGG                ACTGG(1/-1)
BstEII        439  GGTGACC              G^GT[ACGT]ACC
BstPI         439  GGTGACC              G^GT[ACGT]ACC
EagI          138  CGGCCG               C^GGCCG
Eam1105I      367  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          571  CTCTTC               CTCTTC(1/4)
EclXI         138  CGGCCG               C^GGCCG
Eco52I        138  CGGCCG               C^GGCCG
EcoO65I       439  GGTGACC              G^GT[ACGT]ACC
FbaI           17  TGATCA               T^GATCA
KasI          266  GGCGCC               G^GCGCC
Ksp632I       571  CTCTTC               CTCTTC(1/4)
KspI          201  CCGCGG               CCGC^GG
MaeIII        440  GTGAC                ^GT[ACGT]AC
MluNI         581  TGGCCA               TGG^CCA
MscI          581  TGGCCA               TGG^CCA
MslI          297  CATCCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          266  GGCGCC               GG^CGCC
NcoI          578  CCATGG               C^CATGG
PvuII         484  CAGCTG               CAG^CTG
SacII         201  CCGCGG               CCGC^GG
SapI          570  GCTCTTC              GCTCTTC(1/4)
ScaI          635  AGTACT               AGT^ACT
SfaNI         731  GCATC                GCATC(5/9)
SfoI          266  GGCGCC               GGC^GCC
SmaI          714  CCCGGG               CCC^GGG
SmlI          382  CTCAAG               C^T[CT][GA]AG
SspBI         779  TGTACA               T^GTACA
Tsp45I        440  GTGAC                ^GT[GC]AC
XcmI          395  CCAAGGAGAACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         714  CCCGGG               C^CCGGG
XmaI          714  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

Aor51HI       506  AGCGCT               AGC^GCT
              827  AGCGCT              
AvaI           33  CCCGAG               C^[CT]CG[GA]G
              714  CCCGGG              
AvaII         465  GGACC                G^G[AT]CC
              552  GGACC               
BanI          266  GGCGCC               G^G[CT][GA]CC
              738  GGCACC              
BsmAI         287  GAGAC                GTCTC(1/5)
              625  GTCTC               
BsoBI          33  CCCGAG               C^[CT]CG[GA]G
              714  CCCGGG              
BssHII        566  GCGCGC               G^CGCGC
              748  GCGCGC              
BstYI         359  AGATCC               [GA]^GATC[CT]
              524  AGATCT              
DdeI           49  CTGAG                C^T[ACGT]AG
              349  CTCAG               
DsaI          201  CCGCGG               C^C[GA][CT]GG
              578  CCATGG              
Eco47III      506  AGCGCT               AGC^GCT
              827  AGCGCT              
FauI          256  CCCGC                CCCGC(4/6)
              684  GCGGG               
HinfI          40  GACTC                G^A[ACGT]TC
              325  GACTC               
HphI          439  GGTGA                GGTGA(8/7)
              134  TCACC               
MflI          359  AGATCC               [GA]^GATC[CT]
              524  AGATCT              
NlaIV         266  GGCGCC               GG[ACGT]^[ACGT]CC
              738  GGCACC              
PleI           40  GACTC                GAGTC(4/5)
              325  GACTC               
XhoII         359  AGATCC               [GA]^GATC[CT]
              524  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
BbvI        BcnI        BmyI        BsaHI       BsaJI       
BsgI        BsiYI       BslI        Bsp1286I    BsrFI       
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DpnII       EaeI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       ItaI        MaeII       MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      RsaI        Sau3AI      Sau96I      
ScrFI       StyI        TaqI        TseI        TthHB8I     
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