Restriction map for pF1KB5186
 (Linear) (Six-base) MAPSORT of: KIBB5186.seq  Check: 142  from: 1  to: 843



KIBB5186.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0     212     843 
   Size:        212     631
 
AfeI AGC'GCT

Cuts at:      0     508     829     843 
   Size:        508     321      14

  Fragments arranged by size: 

                 508     321      14
 
AflIII A'CryG_T

Cuts at:      0     233     843 
   Size:        233     610
 
AhdI GACnn_n'nnGTC

Cuts at:      0     372     843 
   Size:        372     471
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     544     576     843 
   Size:        544      32     267

  Fragments arranged by size: 

                 544     267      32
 
AvaI C'yCGr_G

Cuts at:      0      33     714     843 
   Size:         33     681     129

  Fragments arranged by size: 

                 681     129      33
 
BanI G'GyrC_C

Cuts at:      0     266     738     843 
   Size:        266     472     105

  Fragments arranged by size: 

                 472     266     105
 
BanII G_rGCy'C

Cuts at:      0     607     843 
   Size:        607     236
 
BbeI G_GCGC'C

Cuts at:      0     270     843 
   Size:        270     573
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      64     843 
   Size:         64     779
 
BclI T'GATC_A

Cuts at:      0      17     843 
   Size:         17     826
 
BglII A'GATC_T

Cuts at:      0     524     843 
   Size:        524     319
 
Bme1580I G_kGCm'C

Cuts at:      0      34     416     843 
   Size:         34     382     427

  Fragments arranged by size: 

                 427     382      34
 
BmgBI CAC'GTC

Cuts at:      0     236     411     645     843 
   Size:        236     175     234     198

  Fragments arranged by size: 

                 236     234     198     175
 
Bpu10I CC'TnA_GC

Cuts at:      0     349     843 
   Size:        349     494
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     843 
   Size:        367     476
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     630     843 
   Size:        630     213
 
BsaHI Gr'CG_yC

Cuts at:      0     152     267     722     843 
   Size:        152     115     455     121

  Fragments arranged by size: 

                 455     152     121     115
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     310     340     843 
   Size:        310      30     503

  Fragments arranged by size: 

                 503     310      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     349     843 
   Size:        349     494
 
BseYI C'CCAG_C

Cuts at:      0     503     592     757     843 
   Size:        503      89     165      86

  Fragments arranged by size: 

                 503     165      89      86
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     598     634     652     843 
   Size:        361     237      36      18     191

  Fragments arranged by size: 

                 361     237     191      36      18
 
BsiEI CG_ry'CG

Cuts at:      0     141     843 
   Size:        141     702
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     281     843 
   Size:        281     562
 
Bsp1286I G_dGCh'C

Cuts at:      0      34     416     607     843 
   Size:         34     382     191     236

  Fragments arranged by size: 

                 382     236     191      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     620     843 
   Size:        620     223
 
BsrFI r'CCGG_y

Cuts at:      0     136     688     752     802     843 
   Size:        136     552      64      50      41

  Fragments arranged by size: 

                 552     136      64      50      41
 
BsrGI T'GTAC_A

Cuts at:      0     779     843 
   Size:        779      64
 
BssHII G'CGCG_C

Cuts at:      0     566     748     843 
   Size:        566     182      95

  Fragments arranged by size: 

                 566     182      95
 
BstEII G'GTnAC_C

Cuts at:      0     439     843 
   Size:        439     404
 
BstYI r'GATC_y

Cuts at:      0     359     524     843 
   Size:        359     165     319

  Fragments arranged by size: 

                 359     319     165
 
BtgI C'CryG_G

Cuts at:      0     201     578     843 
   Size:        201     377     265

  Fragments arranged by size: 

                 377     265     201
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      28     843 
   Size:         28     815
 
EaeI y'GGCC_r

Cuts at:      0       8      59     113     138     530     581     843 
   Size:          8      51      54      25     392      51     262

  Fragments arranged by size: 

                 392     262      54      51      51      25       8
 
EagI C'GGCC_G

Cuts at:      0     138     843 
   Size:        138     705
 
EarI CTCTTCn'nnn_

Cuts at:      0     577     843 
   Size:        577     266
 
HaeII r_GCGC'y

Cuts at:      0     270     510     664     803     831     843 
   Size:        270     240     154     139      28      12

  Fragments arranged by size: 

                 270     240     154     139      28      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     358     390     843 
   Size:        358      32     453

  Fragments arranged by size: 

                 453     358      32
 
KasI G'GCGC_C

Cuts at:      0     266     843 
   Size:        266     577
 
MscI TGG'CCA

Cuts at:      0     583     843 
   Size:        583     260
 
MslI CAynn'nnrTG

Cuts at:      0     301     843 
   Size:        301     542
 
MspA1I CmG'CkG

Cuts at:      0     203     345     486     695     843 
   Size:        203     142     141     209     148

  Fragments arranged by size: 

                 209     203     148     142     141
 
NaeI GCC'GGC

Cuts at:      0     690     754     804     843 
   Size:        690      64      50      39

  Fragments arranged by size: 

                 690      64      50      39
 
NarI GG'CG_CC

Cuts at:      0     267     843 
   Size:        267     576
 
NcoI C'CATG_G

Cuts at:      0     578     843 
   Size:        578     265
 
NgoMIV G'CCGG_C

Cuts at:      0     688     752     802     843 
   Size:        688      64      50      41

  Fragments arranged by size: 

                 688      64      50      41
 
PvuII CAG'CTG

Cuts at:      0     486     843 
   Size:        486     357
 
SacII CC_GC'GG

Cuts at:      0     204     843 
   Size:        204     639
 
SapI GCTCTTCn'nnn_

Cuts at:      0     577     843 
   Size:        577     266
 
ScaI AGT'ACT

Cuts at:      0     637     843 
   Size:        637     206
 
SfoI GGC'GCC

Cuts at:      0     268     843 
   Size:        268     575
 
SmaI CCC'GGG

Cuts at:      0     716     843 
   Size:        716     127
 
SmlI C'TyrA_G

Cuts at:      0     382     843 
   Size:        382     461
 
StyI C'CwwG_G

Cuts at:      0      98     395     578     815     843 
   Size:         98     297     183     237      28

  Fragments arranged by size: 

                 297     237     183      98      28
 
TatI w'GTAC_w

Cuts at:      0     635     779     843 
   Size:        635     144      64

  Fragments arranged by size: 

                 635     144      64
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     402     843 
   Size:        402     441
 
XmaI C'CCGG_G

Cuts at:      0     714     843 
   Size:        714     129
 

 Enzymes that do cut:

     AccI     AfeI   AflIII     AhdI     AloI     AvaI     BanI    BanII
     BbeI    BciVI     BclI    BglII Bme1580I    BmgBI   Bpu10I    BpuEI
     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsmBI
 Bsp1286I    BspMI    BsrFI    BsrGI   BssHII   BstEII    BstYI     BtgI
    BtgZI     EaeI     EagI     EarI    HaeII    Hin4I     KasI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV    PvuII    SacII
     SapI     ScaI     SfoI     SmaI     SmlI     StyI     TatI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BfrBI     BglI
     BlpI     BmrI     BmtI     BplI     BpmI    BsaAI    BsaBI    BsaWI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI     SalI    SanDI     SbfI
    SexAI     SfcI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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