Restriction map for
pF1KB5988
(Circular) (Six-base) MAPSORT of: pF1KB5988.seq Check: 6759 from: 1 to: 4787
pF1KB5988 4787 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AccI GT'mk_AC
Cuts at: 768 1022 1662 1825 768
Size: 254 640 163 3730
Fragments arranged by size:
3730 640 254 163
Acc65I G'GTAC_C
Cuts at: 1803 1803
Size: 4787
AclI AA'CG_TT
Cuts at: 4627 4627
Size: 4787
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 244 656 2612 3044 3791 4059 160
Size: 84 412 1956 432 747 268 888
Fragments arranged by size:
1956 888 747 432 412 268 84
AflIII A'CryG_T
Cuts at: 1065 1467 2139 3243 4220 1065
Size: 402 672 1104 977 1632
Fragments arranged by size:
1632 1104 977 672 402
AgeI A'CCGG_T
Cuts at: 3175 3175
Size: 4787
AlwNI CAG_nnn'CTG
Cuts at: 1267 2092 3659 1267
Size: 825 1567 2395
Fragments arranged by size:
2395 1567 825
ApaLI G'TGCA_C
Cuts at: 3557 3557
Size: 4787
ApoI r'AATT_y
Cuts at: 353 984 1186 1791 4140 4396 353
Size: 631 202 605 2349 256 744
Fragments arranged by size:
2349 744 631 605 256 202
AseI AT'TA_AT
Cuts at: 20 1507 20
Size: 1487 3300
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
AvaI C'yCGr_G
Cuts at: 1807 1807
Size: 4787
BamHI G'GATC_C
Cuts at: 932 1812 932
Size: 880 3907
BanI G'GyrC_C
Cuts at: 1803 2217 2465 2500 1803
Size: 414 248 35 4090
Fragments arranged by size:
4090 414 248 35
BanII G_rGCy'C
Cuts at: 444 827 1801 2831 444
Size: 383 974 1030 2400
Fragments arranged by size:
2400 1030 974 383
BbeI G_GCGC'C
Cuts at: 2221 2469 2221
Size: 248 4539
BbsI GAAGACnn'nnnn_
Cuts at: 284 284
Size: 4787
BciVI GTATCCnnnnn_n'
Cuts at: 1713 2685 3446 4787 1713
Size: 972 761 1341 1713
Fragments arranged by size:
1713 1341 972 761
BclI T'GATC_A
Cuts at: 2307 2307
Size: 4787
BglI GCCn_nnn'nGGC
Cuts at: 1744 1744
Size: 4787
BglII A'GATC_T
Cuts at: 840 1542 2302 4339 840
Size: 702 760 2037 1288
Fragments arranged by size:
2037 1288 760 702
BlpI GC'TnA_GC
Cuts at: 482 1899 482
Size: 1417 3370
Bme1580I G_kGCm'C
Cuts at: 1113 1531 2412 2505 3561 1113
Size: 418 881 93 1056 2339
Fragments arranged by size:
2339 1056 881 418 93
BmrI ACTGGGnnnn_n'
Cuts at: 535 1999 2080 2415 4559 535
Size: 1464 81 335 2144 763
Fragments arranged by size:
2144 1464 763 335 81
BmtI G_CTAG'C
Cuts at: 1588 1588
Size: 4787
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1619 3154 1619
Size: 1535 3252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 986 986
Size: 4787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1963 2842 3334 3632 616
Size: 1347 879 492 298 1771
Fragments arranged by size:
1771 1347 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4491 4491
Size: 4787
BsaAI yAC'GTr
Cuts at: 2140 2140
Size: 4787
BsaBI GATnn'nnATC
Cuts at: 6 2301 4338 6
Size: 2295 2037 455
Fragments arranged by size:
2295 2037 455
BsaHI Gr'CG_yC
Cuts at: 2218 2466 3168 4664 2218
Size: 248 702 1496 2341
Fragments arranged by size:
2341 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1976 2497 3175 3449 3596 4007 1976
Size: 521 678 274 147 411 2756
Fragments arranged by size:
2756 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1162 1739 2212 2970 3547 4043 1162
Size: 577 473 758 577 496 1906
Fragments arranged by size:
1906 758 577 577 496 473
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1441 412
Size: 1029 3758
BsiEI CG_ry'CG
Cuts at: 104 2329 2375 3583 104
Size: 2225 46 1208 1308
Fragments arranged by size:
2225 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 827 1605 1644 1801 2579 2769 3561 827
Size: 778 39 157 778 190 792 2053
Fragments arranged by size:
2053 792 778 778 190 157 39
Bsp1286I G_dGCh'C
Cuts at: 444 827 1113 1531 1605 1644 1801 2412 2505
Size: 383 286 418 74 39 157 611 93
Cuts at: 2505 2579 2769 2831 3561 444
Size: 74 190 62 730 1670
Fragments arranged by size:
1670 730 611 418 383 286 190 157
93 74 74 62 39
BspEI T'CCGG_A
Cuts at: 4007 4007
Size: 4787
BspHI T'CATG_A
Cuts at: 1719 3963 1719
Size: 2244 2543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1837 2051 2353 2734 1837
Size: 214 302 381 3890
Fragments arranged by size:
3890 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3079 3133 4617 4782 50
Size: 3029 54 1484 165 55
Fragments arranged by size:
3029 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 629 2699 629
Size: 2070 2717
BsrFI r'CCGG_y
Cuts at: 2785 2966 3175 2785
Size: 181 209 4397
Fragments arranged by size:
4397 209 181
BsrGI T'GTAC_A
Cuts at: 1563 1563
Size: 4787
BssSI C'ACGA_G
Cuts at: 3058 3416 3058
Size: 358 4429
BstAPI GCAn_nnn'nTGC
Cuts at: 536 988 1108 1267 2164 536
Size: 452 120 159 897 3159
Fragments arranged by size:
3159 897 452 159 120
BstBI TT'CG_AA
Cuts at: 3148 3148
Size: 4787
BstXI CCAn_nnnn'nTGG
Cuts at: 250 250
Size: 4787
BstYI r'GATC_y
Cuts at: 1 840 932 1542 1651 1812 2279 2302 2637
Size: 839 92 610 109 161 467 23 335
Cuts at: 2637 2883 3895 3981 3993 4339 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 839 610 467 449 346 335 246
161 109 92 86 23 12
BtgI C'CryG_G
Cuts at: 107 1451 1625 107
Size: 1344 174 3269
Fragments arranged by size:
3269 1344 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2737 2918 3081 2737
Size: 181 163 4443
Fragments arranged by size:
4443 181 163
BtsI GCAGTG_nn'
Cuts at: 534 1529 2030 534
Size: 995 501 3291
Fragments arranged by size:
3291 995 501
DraI TTT'AAA
Cuts at: 1786 1786
Size: 4787
DraIII CAC_nnn'GTG
Cuts at: 1108 1108
Size: 4787
DrdI GACnn_nn'nnGTC
Cuts at: 288 2493 3351 288
Size: 2205 858 1724
Fragments arranged by size:
2205 1724 858
EaeI y'GGCC_r
Cuts at: 1124 2372 2546 2937 2964 4309 1124
Size: 1248 174 391 27 1345 1602
Fragments arranged by size:
1602 1345 1248 391 174 27
EagI C'GGCC_G
Cuts at: 2372 2372
Size: 4787
EarI CTCTTCn'nnn_
Cuts at: 632 658 2810 3020 632
Size: 26 2152 210 2399
Fragments arranged by size:
2399 2152 210 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2995 3305 3451 4597 2995
Size: 310 146 1146 3185
Fragments arranged by size:
3185 1146 310 146
EcoICRI GAG'CTC
Cuts at: 825 1799 825
Size: 974 3813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 244 656 986 2612 3044 3791 4059 160
Size: 84 412 330 1626 432 747 268 888
Fragments arranged by size:
1626 888 747 432 412 330 268 84
EcoO109I rG'GnC_Cy
Cuts at: 1926 1926
Size: 4787
EcoRI G'AATT_C
Cuts at: 353 1791 353
Size: 1438 3349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 442 502 2806 442
Size: 60 2304 2423
Fragments arranged by size:
2423 2304 60
FspI TGC'GCA
Cuts at: 2568 2568
Size: 4787
HaeII r_GCGC'y
Cuts at: 2221 2469 3491 4470 2221
Size: 248 1022 979 2538
Fragments arranged by size:
2538 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1626 1658 1687 1719 2629 2659 2661 2691 1626
Size: 32 29 32 910 30 2 30 3722
Fragments arranged by size:
3722 910 32 32 30 30 29 2
HincII GTy'rAC
Cuts at: 156 281 1826 156
Size: 125 1545 3117
Fragments arranged by size:
3117 1545 125
KasI G'GCGC_C
Cuts at: 2217 2465 2217
Size: 248 4539
KpnI G_GTAC'C
Cuts at: 1807 1807
Size: 4787
MfeI C'AATT_G
Cuts at: 977 1276 977
Size: 299 4488
MluI A'CGCG_T
Cuts at: 4220 4220
Size: 4787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3458 3642 3458
Size: 184 4603
MscI TGG'CCA
Cuts at: 1126 2548 1126
Size: 1422 3365
MslI CAynn'nnrTG
Cuts at: 1340 2776 2903 1340
Size: 1436 127 3224
Fragments arranged by size:
3224 1436 127
MspA1I CmG'CkG
Cuts at: 151 264 396 741 1162 1898 2212 2572 3585
Size: 113 132 345 421 736 314 360 1013
Cuts at: 3585 3830 151
Size: 245 1108
Fragments arranged by size:
1108 1013 736 421 360 345 314 245
132 113
NaeI GCC'GGC
Cuts at: 2968 2968
Size: 4787
NarI GG'CG_CC
Cuts at: 2218 2466 2218
Size: 248 4539
NcoI C'CATG_G
Cuts at: 107 1451 1625 107
Size: 1344 174 3269
Fragments arranged by size:
3269 1344 174
NdeI CA'TA_TG
Cuts at: 454 1016 454
Size: 562 4225
NgoMIV G'CCGG_C
Cuts at: 2966 2966
Size: 4787
NheI G'CTAG_C
Cuts at: 1584 1584
Size: 4787
NspI r_CATG'y
Cuts at: 1069 1099 1471 1840 2340 3247 1069
Size: 30 372 369 500 907 2609
Fragments arranged by size:
2609 907 500 372 369 30
PciI A'CATG_T
Cuts at: 1065 1467 3243 1065
Size: 402 1776 2609
Fragments arranged by size:
2609 1776 402
PmeI GTTT'AAAC
Cuts at: 1786 1786
Size: 4787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 4755
PshAI GACnn'nnGTC
Cuts at: 1368 1368
Size: 4787
PsiI TTA'TAA
Cuts at: 1138 1138
Size: 4787
PstI C_TGCA'G
Cuts at: 1834 2519 1834
Size: 685 4102
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4787
PvuII CAG'CTG
Cuts at: 151 264 396 741 1162 2212 2572 151
Size: 113 132 345 421 1050 360 2366
Fragments arranged by size:
2366 1050 421 360 345 132 113
RsrII CG'GwC_CG
Cuts at: 2982 2982
Size: 4787
SacI G_AGCT'C
Cuts at: 827 1801 827
Size: 974 3813
SalI G'TCGA_C
Cuts at: 1824 1824
Size: 4787
SapI GCTCTTCn'nnn_
Cuts at: 632 2810 3020 632
Size: 2178 210 2399
Fragments arranged by size:
2399 2178 210
SbfI CC_TGCA'GG
Cuts at: 1834 1834
Size: 4787
ScaI AGT'ACT
Cuts at: 4304 4304
Size: 4787
SfcI C'TryA_G
Cuts at: 33 736 955 1830 2515 3508 3699 33
Size: 703 219 875 685 993 191 1121
Fragments arranged by size:
1121 993 875 703 685 219 191
SfoI GGC'GCC
Cuts at: 2219 2467 2219
Size: 248 4539
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
SmaI CCC'GGG
Cuts at: 1809 1809
Size: 4787
SmlI C'TyrA_G
Cuts at: 595 1942 2857 3349 3611 595
Size: 1347 915 492 262 1771
Fragments arranged by size:
1771 1347 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1459 67
Size: 1392 3395
SphI G_CATG'C
Cuts at: 1099 1840 2340 1099
Size: 741 500 3546
Fragments arranged by size:
3546 741 500
StuI AGG'CCT
Cuts at: 755 1747 755
Size: 992 3795
StyI C'CwwG_G
Cuts at: 107 1451 1625 1921 2275 107
Size: 1344 174 296 354 2619
Fragments arranged by size:
2619 1344 354 296 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 487 2370 2503 2962 3171 487
Size: 1883 133 459 209 2103
Fragments arranged by size:
2103 1883 459 209 133
TatI w'GTAC_w
Cuts at: 622 1089 1563 1674 4302 622
Size: 467 474 111 2628 1107
Fragments arranged by size:
2628 1107 474 467 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4304 4336 4304
Size: 32 4755
Tth111I GACn'n_nGTC
Cuts at: 2584 2584
Size: 4787
XbaI T'CTAG_A
Cuts at: 1545 1692 1818 1545
Size: 147 126 4514
Fragments arranged by size:
4514 147 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2282 2282
Size: 4787
XmaI C'CCGG_G
Cuts at: 1807 1807
Size: 4787
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NspI PciI PmeI PpiI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI