Restriction map for pF1KE0538
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BsrFI       BsrI        BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       BstUI       
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HincII      HindII      
HinfI       HpaI        HpaII       KasI        KspI        
MaeI        MamI        MfeI        MluI        MroI        
MseI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
TfiI        Tru9I       Tth111I     Van91I      XbaI        
XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I        490  GGTACC               G^GTACC
AccI          345  GTATAC               GT^[AC][GT]AC
AciI          243  GCGG                 CCGC(-3/-1)
AlwI          375  GATCC                GGATC(4/5)
AlwNI         145  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       504  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       490  GGTACC               G^GTACC
AvaI          586  CTCGAG               C^[CT]CG[GA]G
AvaII          33  GGACC                G^G[AT]CC
BalI          392  TGGCCA               TGG^CCA
BanI          490  GGTACC               G^G[CT][GA]CC
BanII         583  GAGCTC               G[GA]GC[CT]^C
BbsI            7  GAAGAC               GAAGAC(2/6)
BcgI          641  GCAGCAGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI           7  GAAGAC               GAAGAC(2/6)
BsaAI         156  CACGTA               [CT]AC^GT[GA]
BsaI          652  GAGACC               GGTCTC(1/5)
BsiWI         158  CGTACG               C^GTACG
BsiYI         657  CCCATCAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          657  CCCATCAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI         474  CGTCTC               CGTCTC(1/5)
BsmFI         224  GGGAC                GGGAC(10/14)
BsoBI         586  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      173  TGTACA               T^GTACA
BsrBI         241  GAGCGG               CCGCTC(-3/-3)
BsrGI         173  TGTACA               T^GTACA
Bst1107I      345  GTATAC               GTA^TAC
BstYI         374  AGATCC               [GA]^GATC[CT]
BstZ17I       345  GTATAC               GTA^TAC
CfoI           89  GCGC                 GCG^C
DdeI          334  CTGAG                C^T[ACGT]AG
DsaI          188  CCACGG               C^C[GA][CT]GG
EaeI          392  TGGCCA               [CT]^GGCC[GA]
EcoT14I        36  CCAAGG               C^C[AT][AT]GG
HhaI           89  GCGC                 GCG^C
HinP1I         89  GCGC                 G^CGC
HindIII       432  AAGCTT               A^AGCTT
HphI          320  TCACC                GGTGA(8/7)
KpnI          490  GGTACC               GGTAC^C
MflI          374  AGATCC               [GA]^GATC[CT]
MluNI         392  TGGCCA               TGG^CCA
MscI          392  TGGCCA               TGG^CCA
MspA1I        148  CAGCTG               C[AC]G^C[GT]G
PaeR7I        586  CTCGAG               C^TCGAG
PvuII         148  CAGCTG               CAG^CTG
SacI          583  GAGCTC               GAGCT^C
SapI          507  GCTCTTC              GCTCTTC(1/4)
SexAI         340  ACCTGGT              A^CC[AT]GGT
SfcI          324  CTATAG               C^T[GA][CT]AG
SmlI          586  CTCGAG               C^T[CT][GA]AG
SspBI         173  TGTACA               T^GTACA
StyI           36  CCAAGG               C^C[AT][AT]GG
TspRI          92  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoI          586  CTCGAG               C^TCGAG
XhoII         374  AGATCC               [GA]^GATC[CT]
XmnI          504  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AspHI         336  GAGCAC               G[AT]GC[AT]^C
              583  GAGCTC              
BmyI          336  GAGCAC               G[AGT]GC[ACT]^C
              583  GAGCTC              
BseRI         613  GAGGAG               GAGGAG(10/8)
              664  GAGGAG              
BsiHKAI       336  GAGCAC               G[AT]GC[AT]^C
              583  GAGCTC              
Bsp1286I      336  GAGCAC               G[AGT]GC[ACT]^C
              583  GAGCTC              
BstXI         104  CCAAACTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              206  CCATCCTTGTGG        
Cfr13I         33  GGACC                G^G[ACGT]CC
              279  GGCCC               
DpnII          69  GATC                 ^GATC
              375  GATC                
EarI          508  CTCTTC               CTCTTC(1/4)
              610  GAAGAG              
EcoT22I       256  ATGCAT               ATGCA^T
              381  ATGCAT              
Ksp632I       508  CTCTTC               CTCTTC(1/4)
              610  GAAGAG              
MaeIII        319  GTCAC                ^GT[ACGT]AC
              421  GTGAC               
MboI           69  GATC                 ^GATC
              375  GATC                
MwoI           89  GCGCAGTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              141  GCCACAGCAGC         
NdeII          69  GATC                 ^GATC
              375  GATC                
NsiI          256  ATGCAT               ATGCA^T
              381  ATGCAT              
NspI          254  GCATGC               [GA]CATG^[CT]
              258  GCATGC              
Sau3AI         69  GATC                 ^GATC
              375  GATC                
Sau96I         33  GGACC                G^G[ACGT]CC
              279  GGCCC               
SphI          254  GCATGC               GCATG^C
              258  GCATGC              
Tsp45I        319  GTCAC                ^GT[GC]AC
              421  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AfaI        AluI        BbvI        BsaJI       BsmAI       
BssKI       BstF5I      BstNI       Cac8I       EcoRII      
Fnu4HI      FokI        HaeIII      ItaI        MaeII       
MboII       MnlI        MslI        MvaI        NlaIII      
NlaIV       RsaI        ScrFI       SfaNI       TaqI        
TseI        Tsp509I     TthHB8I     
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