Restriction map for pF1KE0538
 (Linear) (Six-base) MAPSORT of: KIEE0538.seq  Check: 9651  from: 1  to: 687



KIEE0538.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AccI GT'mk_AC

Cuts at:      0     346     687 
   Size:        346     341
 
Acc65I G'GTAC_C

Cuts at:      0     490     687 
   Size:        490     197
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     579     687 
   Size:        579     108
 
AlwNI CAG_nnn'CTG

Cuts at:      0     150     687 
   Size:        150     537
 
AvaI C'yCGr_G

Cuts at:      0     586     687 
   Size:        586     101
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     482     515     687 
   Size:        482      33     172

  Fragments arranged by size: 

                 482     172      33
 
BanI G'GyrC_C

Cuts at:      0     490     687 
   Size:        490     197
 
BanII G_rGCy'C

Cuts at:      0     587     687 
   Size:        587     100
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      14     687 
   Size:         14     673
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     630     664     687 
   Size:        630      34      23

  Fragments arranged by size: 

                 630      34      23
 
BfrBI ATG'CAT

Cuts at:      0     258     383     687 
   Size:        258     125     304

  Fragments arranged by size: 

                 304     258     125
 
BmgBI CAC'GTC

Cuts at:      0     621     687 
   Size:        621      66
 
Bpu10I CC'TnA_GC

Cuts at:      0     334     687 
   Size:        334     353
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     646     687 
   Size:        646      41
 
BsaAI yAC'GTr

Cuts at:      0     158     687 
   Size:        158     529
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     628     679     687 
   Size:        628      51       8

  Fragments arranged by size: 

                 628      51       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     340     587     687 
   Size:        340     247     100

  Fragments arranged by size: 

                 340     247     100
 
BsiWI C'GTAC_G

Cuts at:      0     158     687 
   Size:        158     529
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     480     687 
   Size:        480     207
 
Bsp1286I G_dGCh'C

Cuts at:      0     340     587     687 
   Size:        340     247     100

  Fragments arranged by size: 

                 340     247     100
 
BsrBI CCG'CTC

Cuts at:      0     243     687 
   Size:        243     444
 
BsrGI T'GTAC_A

Cuts at:      0     173     687 
   Size:        173     514
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     111     213     687 
   Size:        111     102     474

  Fragments arranged by size: 

                 474     111     102
 
BstYI r'GATC_y

Cuts at:      0     374     687 
   Size:        374     313
 
BstZ17I GTA'TAC

Cuts at:      0     347     687 
   Size:        347     340
 
BtgI C'CryG_G

Cuts at:      0     188     687 
   Size:        188     499
 
BtsI GCAGTG_nn'

Cuts at:      0      98     687 
   Size:         98     589
 
EaeI y'GGCC_r

Cuts at:      0     392     687 
   Size:        392     295
 
EarI CTCTTCn'nnn_

Cuts at:      0     514     605     687 
   Size:        514      91      82

  Fragments arranged by size: 

                 514      91      82
 
EcoICRI GAG'CTC

Cuts at:      0     585     687 
   Size:        585     102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     579     687 
   Size:        579     108
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     643     675     687 
   Size:        643      32      12

  Fragments arranged by size: 

                 643      32      12
 
HindIII A'AGCT_T

Cuts at:      0     432     687 
   Size:        432     255
 
KpnI G_GTAC'C

Cuts at:      0     494     687 
   Size:        494     193
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      11     687 
   Size:         11     676
 
MscI TGG'CCA

Cuts at:      0     394     687 
   Size:        394     293
 
MslI CAynn'nnrTG

Cuts at:      0     211     295     388     418     687 
   Size:        211      84      93      30     269

  Fragments arranged by size: 

                 269     211      93      84      30
 
MspA1I CmG'CkG

Cuts at:      0     150     687 
   Size:        150     537
 
NsiI A_TGCA'T

Cuts at:      0     260     385     687 
   Size:        260     125     302

  Fragments arranged by size: 

                 302     260     125
 
NspI r_CATG'y

Cuts at:      0     258     262     687 
   Size:        258       4     425

  Fragments arranged by size: 

                 425     258       4
 
PvuII CAG'CTG

Cuts at:      0     150     687 
   Size:        150     537
 
SacI G_AGCT'C

Cuts at:      0     587     687 
   Size:        587     100
 
SapI GCTCTTCn'nnn_

Cuts at:      0     514     687 
   Size:        514     173
 
SexAI A'CCwGG_T

Cuts at:      0     340     687 
   Size:        340     347
 
SfcI C'TryA_G

Cuts at:      0     324     687 
   Size:        324     363
 
SmlI C'TyrA_G

Cuts at:      0     586     687 
   Size:        586     101
 
SphI G_CATG'C

Cuts at:      0     258     262     687 
   Size:        258       4     425

  Fragments arranged by size: 

                 425     258       4
 
StyI C'CwwG_G

Cuts at:      0      36     687 
   Size:         36     651
 
TatI w'GTAC_w

Cuts at:      0     173     687 
   Size:        173     514
 
XhoI C'TCGA_G

Cuts at:      0     586     687 
   Size:        586     101
 
XmnI GAAnn'nnTTC

Cuts at:      0     508     687 
   Size:        508     179
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AlwNI     AvaI     BaeI     BanI    BanII
     BbsI     BcgI    BfrBI    BmgBI   Bpu10I     BsaI    BsaAI    BseRI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BsrBI    BsrGI    BstXI    BstYI
  BstZ17I     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI    Hin4I
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NsiI     NspI
    PvuII     SacI     SapI    SexAI     SfcI     SmlI     SphI     StyI
     TatI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII    BamHI     BbeI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI     BsmI
    BspEI    BspHI    BspMI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XmaI
     ZraI
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