Restriction map for pF1KE0117
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BclI        BcnI        BfaI        BfrI        
BglI        BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstF5I      BstPI       BstZ17I     Bsu36I      Cac8I       
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FokI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        MaeI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AflIII        327  ACACGT               A^C[GA][CT]GT
AseI          472  ATTAAT               AT^TAAT
AsnI          472  ATTAAT               AT^TAAT
Asp700I       483  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          461  CCCGAG               C^[CT]CG[GA]G
AvaII         284  GGTCC                G^G[AT]CC
BbsI          254  GTCTTC               GAAGAC(2/6)
BcgI          393  GCAAGTGACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         182  AGATCT               A^GATCT
BpmI          178  CTCCAG               CTGGAG(16/14)
BpuAI         254  GTCTTC               GAAGAC(2/6)
BsaBI         537  GATTACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          316  GAGACC               GGTCTC(1/5)
BseRI         624  GAGGAG               GAGGAG(10/8)
BsoBI         461  CCCGAG               C^[CT]CG[GA]G
BspMI         281  GCAGGT               ACCTGC(4/8)
BssSI         406  CACGAG               CACGAG(-5/-1)
BstAPI         59  GCAAGTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         470  CCATTAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          227  GCGC                 GCG^C
Cfr13I        284  GGTCC                G^G[ACGT]CC
DdeI          243  CTCAG                C^T[ACGT]AG
DraII         283  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoNI         230  CCTACTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      283  AGGTCCT              [GA]G^G[ACGT]CC[CT]
HapII          27  CCGG                 C^CGG
HhaI          227  GCGC                 GCG^C
HinP1I        227  GCGC                 G^CGC
HpaII          27  CCGG                 C^CGG
KspI          194  CCGCGG               CCGC^GG
MamI          537  GATTACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        194  CCGCGG               C[AC]G^C[GT]G
MspI           27  CCGG                 C^CGG
PleI          399  GACTC                GAGTC(4/5)
PpuMI         283  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI         472  ATTAAT               AT^TAAT
SacII         194  CCGCGG               CCGC^GG
Sau96I        284  GGTCC                G^G[ACGT]CC
SfcI          234  CTACAG               C^T[GA][CT]AG
TaqI          155  TCGA                 T^CGA
TfiI           46  GATTC                G^A[AT]TC
Tsp509I       345  AATT                 ^AATT
TspRI          53  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       155  TCGA                 T^CGA
XcmI          116  CCACCGTGGGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          483  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         195  CGCG                 CG^CG
              226  CGCG                
AluI          176  AGCT                 AG^CT
              518  AGCT                
AlwI           72  GATCC                GGATC(4/5)
              215  GATCC               
BbvI          424  GCTGC                GCAGC(8/12)
              427  GCTGC               
BciVI         189  GGATAC               GTATCC(6/5)
              381  GGATAC              
BsmAI         316  GAGAC                GTCTC(1/5)
              506  GAGAC               
BsrI          420  ACTGG                ACTGG(1/-1)
               21  CCAGT               
BstUI         195  CGCG                 CG^CG
              226  CGCG                
BstYI         182  AGATCT               [GA]^GATC[CT]
              214  AGATCC              
DsaI          119  CCGTGG               C^C[GA][CT]GG
              194  CCGCGG              
FauI          140  CCCGC                CCCGC(4/6)
              196  GCGGG               
HaeIII          6  GGCC                 GG^CC
              411  GGCC                
HinfI          46  GATTC                G^A[ACGT]TC
              399  GACTC               
HphI          564  GGTGA                GGTGA(8/7)
               83  TCACC               
MaeII         329  ACGT                 A^CGT
              454  ACGT                
MflI          182  AGATCT               [GA]^GATC[CT]
              214  AGATCC              
MseI          473  TTAA                 T^TAA
              524  TTAA                
MslI          367  CACAAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              471  CATTAATGTG          
MvnI          195  CGCG                 CG^CG
              226  CGCG                
NlaIII        301  CATG                 CATG^
              438  CATG                
SfaNI         466  GATGC                GCATC(5/9)
              170  GCATC               
Tru9I         473  TTAA                 T^TAA
              524  TTAA                
TseI          424  GCTGC                G^C[AT]GC
              427  GCTGC               
XhoII         182  AGATCT               [GA]^GATC[CT]
              214  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AfaI        BsaJI       BsiYI       BslI        
BssKI       BstNI       DpnII       EarI        EcoRII      
Fnu4HI      ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
RsaI        Sau3AI      ScrFI       Tsp45I      
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