Restriction map for pF1KB3558
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AlwI        AocI        ApoI        
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfaI        BfrI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaWI       BseAI       
BseRI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeIII      MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tsp45I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII          69  GACGTC               GACGT^C
AccI           72  GTCGAC               GT^[AC][GT]AC
AcyI           69  GACGTC               G[GA]^CG[CT]C
AhdI          718  GACCTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        763  GTGCAC               G^TGCAC
ApaI          223  GGGCCC               GGGCC^C
ApaLI         763  GTGCAC               G^TGCAC
Asp700I       828  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         718  GACCTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          241  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          955  CCCGAG               C^[CT]CG[GA]G
BalI          770  TGGCCA               TGG^CCA
BanI          583  GGTGCC               G^G[CT][GA]CC
BbsI          405  GAAGAC               GAAGAC(2/6)
BclI          593  TGATCA               T^GATCA
BglI          850  GCCGCCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         524  AGATCT               A^GATCT
BpuAI         405  GAAGAC               GAAGAC(2/6)
BsaAI         397  TACGTG               [CT]AC^GT[GA]
BsaHI          69  GACGTC               G[GA]^CG[CT]C
BsgI          622  GTGCAG               GTGCAG(16/14)
BsiEI         240  CGACCG               CG[GA][CT]^CG
BsoBI         955  CCCGAG               C^[CT]CG[GA]G
BsrDI         920  GCAATG               GCAATG(2/0)
BsrFI          10  GCCGGT               [GA]^CCGG[CT]
BssSI         700  CTCGTG               CACGAG(-5/-1)
BstAPI        867  GCAGTCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         524  AGATCT               [GA]^GATC[CT]
Cfr10I         10  GCCGGT               [GA]^CCGG[CT]
Eam1105I      718  GACCTTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI          593  TGATCA               T^GATCA
Hin1I          69  GACGTC               G[GA]^CG[CT]C
HincII         72  GTCGAC               GT[CT]^[GA]AC
HindII         72  GTCGAC               GT[CT]^[GA]AC
MflI          524  AGATCT               [GA]^GATC[CT]
MluNI         770  TGGCCA               TGG^CCA
MscI          770  TGGCCA               TGG^CCA
NcoI          116  CCATGG               C^CATGG
NspI          736  GCATGT               [GA]CATG^[CT]
PflFI         241  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         641  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          948  GAGTC                GAGTC(4/5)
PstI          877  CTGCAG               CTGCA^G
PvuII         536  CAGCTG               CAG^CTG
SalI           72  GTCGAC               G^TCGAC
ScaI          791  AGTACT               AGT^ACT
SgrAI           9  CGCCGGTG             C[GA]^CCGG[CT]G
Tsp509I       483  AATT                 ^AATT
Tth111I       241  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        641  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         524  AGATCT               [GA]^GATC[CT]
XmnI          828  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AfaI          270  GTAC                 GT^AC
              792  GTAC                
Aor51HI       322  AGCGCT               AGC^GCT
              488  AGCGCT              
AspHI         301  GAGCAC               G[AT]GC[AT]^C
              763  GTGCAC              
BcnI          579  CCGGG                CC^[GC]GG
              905  CCCGG               
BsaI          926  GAGACC               GGTCTC(1/5)
              958  GAGACC              
BsiHKAI       301  GAGCAC               G[AT]GC[AT]^C
              763  GTGCAC              
BsmI          832  GCATTC               GAATGC(1/-1)
              859  GCATTC              
BspMI          92  GCAGGT               ACCTGC(4/8)
              315  GCAGGT              
BstXI         371  CCAAGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              464  CCATTGGGGTGG        
DsaI          116  CCATGG               C^C[GA][CT]GG
              309  CCACGG              
EaeI          770  TGGCCA               [CT]^GGCC[GA]
              806  CGGCCA              
Eco47III      322  AGCGCT               AGC^GCT
              488  AGCGCT              
EcoT14I       116  CCATGG               C^C[AT][AT]GG
              371  CCAAGG              
HaeII         322  AGCGCT               [GA]GCGC^[CT]
              488  AGCGCT              
HgaI           32  GACGC                GACGC(5/10)
              757  GACGC               
MslI          465  CATTGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              615  CACTGACGTG          
NciI          579  CCGGG                CC^[GC]GG
              905  CCCGG               
NlaIII        117  CATG                 CATG^
              737  CATG                
RsaI          270  GTAC                 GT^AC
              792  GTAC                
SfcI          513  CTATAG               C^T[GA][CT]AG
              877  CTGCAG              
StyI          116  CCATGG               C^C[AT][AT]GG
              371  CCAAGG              
TfiI           78  GATTC                G^A[AT]TC
              507  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AvaII       
BanII       BbvI        BmyI        BpmI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIV       
PpuMI       Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TseI        TspRI       TthHB8I     XcmI        
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