Restriction map for pF1KB3558
 (Linear) (Six-base) MAPSORT of: KIBB3558.seq  Check: 764  from: 1  to: 999



KIBB3558.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AatII G_ACGT'C

Cuts at:      0      73     999 
   Size:         73     926
 
AccI GT'mk_AC

Cuts at:      0      73     999 
   Size:         73     926
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     822     999 
   Size:        822     177
 
AfeI AGC'GCT

Cuts at:      0     324     490     999 
   Size:        324     166     509

  Fragments arranged by size: 

                 509     324     166
 
AhdI GACnn_n'nnGTC

Cuts at:      0     723     999 
   Size:        723     276
 
AleI CACnn'nnGTG

Cuts at:      0     619     999 
   Size:        619     380
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     702     917     999 
   Size:        702     215      82

  Fragments arranged by size: 

                 702     215      82
 
AlwNI CAG_nnn'CTG

Cuts at:      0     630     760     885     999 
   Size:        630     130     125     114

  Fragments arranged by size: 

                 630     130     125     114
 
ApaI G_GGCC'C

Cuts at:      0     227     999 
   Size:        227     772
 
ApaLI G'TGCA_C

Cuts at:      0     763     999 
   Size:        763     236
 
AvaI C'yCGr_G

Cuts at:      0     955     999 
   Size:        955      44
 
BanI G'GyrC_C

Cuts at:      0     583     999 
   Size:        583     416
 
BanII G_rGCy'C

Cuts at:      0     164     227     362     999 
   Size:        164      63     135     637

  Fragments arranged by size: 

                 637     164     135      63
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     412     999 
   Size:        412     587
 
BclI T'GATC_A

Cuts at:      0     593     999 
   Size:        593     406
 
BglI GCCn_nnn'nGGC

Cuts at:      0     856     999 
   Size:        856     143
 
BglII A'GATC_T

Cuts at:      0     524     999 
   Size:        524     475
 
Bme1580I G_kGCm'C

Cuts at:      0     227     588     767     906     999 
   Size:        227     361     179     139      93

  Fragments arranged by size: 

                 361     227     179     139      93
 
BmgBI CAC'GTC

Cuts at:      0     621     999 
   Size:        621     378
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     699     999 
   Size:        699     300
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505     652     895     907     966     999 
   Size:        505     147     243      12      59      33

  Fragments arranged by size: 

                 505     243     147      59      33      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     138     295     999 
   Size:        138     157     704

  Fragments arranged by size: 

                 704     157     138
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     920     952     999 
   Size:        920      32      47

  Fragments arranged by size: 

                 920      47      32
 
BsaAI yAC'GTr

Cuts at:      0     399     999 
   Size:        399     600
 
BsaHI Gr'CG_yC

Cuts at:      0      70     999 
   Size:         70     929
 
BseYI C'CCAG_C

Cuts at:      0     164     502     538     999 
   Size:        164     338      36     461

  Fragments arranged by size: 

                 461     338     164      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     643     999 
   Size:        643     356
 
BsiEI CG_ry'CG

Cuts at:      0     243     999 
   Size:        243     756
 
BsiHKAI G_wGCw'C

Cuts at:      0     305     767     999 
   Size:        305     462     232

  Fragments arranged by size: 

                 462     305     232
 
BsmI GAATG_Cn'

Cuts at:      0     832     859     999 
   Size:        832      27     140

  Fragments arranged by size: 

                 832     140      27
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     227     305     362     588     767     906     999 
   Size:        164      63      78      57     226     179     139      93

  Fragments arranged by size: 

                 226     179     164     139      93      78      63      57
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      83     306     999 
   Size:         83     223     693

  Fragments arranged by size: 

                 693     223      83
 
BsrDI GCAATG_nn'

Cuts at:      0     927     999 
   Size:        927      72
 
BsrFI r'CCGG_y

Cuts at:      0      10     999 
   Size:         10     989
 
BssSI C'ACGA_G

Cuts at:      0     700     999 
   Size:        700     299
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     873     999 
   Size:        873     126
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     378     471     999 
   Size:        378      93     528

  Fragments arranged by size: 

                 528     378      93
 
BstYI r'GATC_y

Cuts at:      0     524     999 
   Size:        524     475
 
BtgI C'CryG_G

Cuts at:      0     116     309     999 
   Size:        116     193     690

  Fragments arranged by size: 

                 690     193     116
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     164     234     546     999 
   Size:        164      70     312     453

  Fragments arranged by size: 

                 453     312     164      70
 
BtsI GCAGTG_nn'

Cuts at:      0     652     906     999 
   Size:        652     254      93

  Fragments arranged by size: 

                 652     254      93
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     409     444     999 
   Size:        409      35     555

  Fragments arranged by size: 

                 555     409      35
 
EaeI y'GGCC_r

Cuts at:      0     770     806     999 
   Size:        770      36     193

  Fragments arranged by size: 

                 770     193      36
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     158     941     999 
   Size:        158     783      58

  Fragments arranged by size: 

                 783     158      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505     652     822     895     907     966     999 
   Size:        505     147     170      73      12      59      33

  Fragments arranged by size: 

                 505     170     147      73      59      33      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0      95     203     223     224     717     882     977     999 
   Size:         95     108      20       1     493     165      95      22

  Fragments arranged by size: 

                 493     165     108      95      95      22      20       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     852     999 
   Size:        852     147
 
HaeII r_GCGC'y

Cuts at:      0     326     492     999 
   Size:        326     166     507

  Fragments arranged by size: 

                 507     326     166
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     709     741     781     813     917     949     999 
   Size:        709      32      40      32     104      32      50

  Fragments arranged by size: 

                 709     104      50      40      32      32      32
 
HincII GTy'rAC

Cuts at:      0      74     999 
   Size:         74     925
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     632     999 
   Size:         73     559     367

  Fragments arranged by size: 

                 559     367      73
 
MscI TGG'CCA

Cuts at:      0     772     999 
   Size:        772     227
 
MslI CAynn'nnrTG

Cuts at:      0     469     619     999 
   Size:        469     150     380

  Fragments arranged by size: 

                 469     380     150
 
MspA1I CmG'CkG

Cuts at:      0      40     133     538     999 
   Size:         40      93     405     461

  Fragments arranged by size: 

                 461     405      93      40
 
NcoI C'CATG_G

Cuts at:      0     116     999 
   Size:        116     883
 
NspI r_CATG'y

Cuts at:      0     740     999 
   Size:        740     259
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     647     999 
   Size:        647     352
 
PpuMI rG'GwC_Cy

Cuts at:      0      95     203     717     882     999 
   Size:         95     108     514     165     117

  Fragments arranged by size: 

                 514     165     117     108      95
 
PspOMI G'GGCC_C

Cuts at:      0     223     999 
   Size:        223     776
 
PstI C_TGCA'G

Cuts at:      0     881     999 
   Size:        881     118
 
PvuII CAG'CTG

Cuts at:      0     538     999 
   Size:        538     461
 
SalI G'TCGA_C

Cuts at:      0      72     999 
   Size:         72     927
 
ScaI AGT'ACT

Cuts at:      0     793     999 
   Size:        793     206
 
SfcI C'TryA_G

Cuts at:      0     513     877     999 
   Size:        513     364     122

  Fragments arranged by size: 

                 513     364     122
 
SgrAI Cr'CCGG_yG

Cuts at:      0      10     999 
   Size:         10     989
 
StyI C'CwwG_G

Cuts at:      0     116     371     999 
   Size:        116     255     628

  Fragments arranged by size: 

                 628     255     116
 
TatI w'GTAC_w

Cuts at:      0     791     999 
   Size:        791     208
 
Tth111I GACn'n_nGTC

Cuts at:      0     244     999 
   Size:        244     755
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     378     468     648     919     999 
   Size:        378      90     180     271      80

  Fragments arranged by size: 

                 378     271     180      90      80
 
XmnI GAAnn'nnTTC

Cuts at:      0     832     999 
   Size:        832     167
 
ZraI GAC'GTC

Cuts at:      0      71     999 
   Size:         71     928
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     AvaI     BanI    BanII     BbsI     BclI     BglI
    BglII Bme1580I    BmgBI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BstYI     BtgI    BtgZI
     BtsI    CspCI     EaeI     EciI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NcoI     NspI
    PflMI    PpuMI   PspOMI     PstI    PvuII     SalI     ScaI     SfcI
    SgrAI     StyI     TatI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII   AflIII     AgeI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BlpI     BmrI     BmtI    BpuEI    BsaBI
    BsaWI    BsaXI    BseRI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     ClaI     DraI
   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI     XbaI     XhoI     XmaI
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