Restriction map for pF1KB6862
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
RcaI        RsrII       SalI        ScaI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AclI          382  AACGTT               AA^CGTT
AocI          540  CCTAAGG              CC^T[ACGT]AGG
Asp700I       480  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         296  GGATCC               G^GATCC
BbeI          193  GGCGCC               GGCGC^C
BsaI          184  GAGACC               GGTCTC(1/5)
BsgI          334  CTGCAC               GTGCAG(16/14)
BsmAI         184  GAGAC                GTCTC(1/5)
BsmFI         549  GTCCC                GGGAC(10/14)
BspMI         514  ACCTGC               ACCTGC(4/8)
BsrBI         577  GAGCGG               CCGCTC(-3/-3)
BstXI         270  CCAGGGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         296  GGATCC               [GA]^GATC[CT]
Bsu36I        540  CCTAAGG              CC^T[ACGT]AGG
DpnII         297  GATC                 ^GATC
DrdI          160  GACAAGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          484  CTCTTC               CTCTTC(1/4)
Eco81I        540  CCTAAGG              CC^T[ACGT]AGG
EcoNI         561  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         193  GGCGCC               [GA]GCGC^[CT]
HinfI         547  GAGTC                G^A[ACGT]TC
KasI          193  GGCGCC               G^GCGCC
Ksp632I       484  CTCTTC               CTCTTC(1/4)
KspI          118  CCGCGG               CCGC^GG
MaeIII        376  GTCAC                ^GT[ACGT]AC
MboI          297  GATC                 ^GATC
MboII         485  TCTTC                GAAGA(8/7)
MflI          296  GGATCC               [GA]^GATC[CT]
NarI          193  GGCGCC               GG^CGCC
NcoI          137  CCATGG               C^CATGG
NdeII         297  GATC                 ^GATC
NruI          368  TCGCGA               TCG^CGA
PleI          547  GAGTC                GAGTC(4/5)
Psp1406I      382  AACGTT               AA^CGTT
SacII         118  CCGCGG               CCGC^GG
SapI          483  GCTCTTC              GCTCTTC(1/4)
Sau3AI        297  GATC                 ^GATC
SbfI          231  CCTGCAGG             CCTGCA^GG
SexAI         560  ACCTGGT              A^CC[AT]GGT
SfoI          193  GGCGCC               GGC^GCC
SmlI           58  CTCAAG               C^T[CT][GA]AG
Sse8387I      231  CCTGCAGG             CCTGCA^GG
StuI          330  AGGCCT               AGG^CCT
Tsp45I        376  GTCAC                ^GT[GC]AC
TspRI         443  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         296  GGATCC               [GA]^GATC[CT]
XmnI          480  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI          408  GTAC                 GT^AC
              435  GTAC                
AlwI          297  GATCC                GGATC(4/5)
              296  GGATC               
AspHI          55  GAGCTC               G[AT]GC[AT]^C
              199  GAGCTC              
BanI           87  GGTGCC               G^G[CT][GA]CC
              193  GGCGCC              
BcnI          245  CCGGG                CC^[GC]GG
              354  CCGGG               
BsiHKAI        55  GAGCTC               G[AT]GC[AT]^C
              199  GAGCTC              
BsrI          509  ACTGG                ACTGG(1/-1)
              552  CCAGT               
DdeI          311  CTCAG                C^T[ACGT]AG
              541  CTAAG               
DsaI          118  CCGCGG               C^C[GA][CT]GG
              137  CCATGG              
EaeI          242  TGGCCG               [CT]^GGCC[GA]
              260  CGGCCA              
EcoT14I       137  CCATGG               C^C[AT][AT]GG
              146  CCTTGG              
HapII         245  CCGG                 C^CGG
              354  CCGG                
HgaI           16  GACGC                GACGC(5/10)
              142  GACGC               
HpaII         245  CCGG                 C^CGG
              354  CCGG                
HphI          155  TCACC                GGTGA(8/7)
              203  TCACC               
MaeII         212  ACGT                 A^CGT
              383  ACGT                
MspI          245  CCGG                 C^CGG
              354  CCGG                
NciI          245  CCGGG                CC^[GC]GG
              354  CCGGG               
NlaIII        138  CATG                 CATG^
              225  CATG                
PpuMI         416  AGGTCCT              [GA]G^G[AT]CC[CT]
              569  AGGACCT             
PstI          232  CTGCAG               CTGCA^G
              253  CTGCAG              
PvuII         250  CAGCTG               CAG^CTG
              290  CAGCTG              
RsaI          408  GTAC                 GT^AC
              435  GTAC                
SacI           55  GAGCTC               GAGCT^C
              199  GAGCTC              
SfcI          232  CTGCAG               C^T[GA][CT]AG
              253  CTGCAG              
StyI          137  CCATGG               C^C[AT][AT]GG
              146  CCTTGG              
XcmI          137  CCATGGACGCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              499  CCAGCCTGGAACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AvaII       
BanII       BbvI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsiYI       BslI        Bsp1286I    
BssHII      BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HhaI        Hin1I       HinP1I      ItaI        MnlI        
MspA1I      MvaI        MvnI        MwoI        NlaIV       
Sau96I      ScrFI       SfaNI       TseI        
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