Restriction map for pF1KB6862
 (Linear) (Six-base) MAPSORT of: KIBB6862.seq  Check: 7487  from: 1  to: 585



KIBB6862.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AclI AA'CG_TT

Cuts at:      0     383     585 
   Size:        383     202
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     471     585 
   Size:         94     377     114

  Fragments arranged by size: 

                 377     114      94
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     374     541     585 
   Size:        374     167      44

  Fragments arranged by size: 

                 374     167      44
 
BamHI G'GATC_C

Cuts at:      0     296     585 
   Size:        296     289
 
BanI G'GyrC_C

Cuts at:      0      87     193     585 
   Size:         87     106     392

  Fragments arranged by size: 

                 392     106      87
 
BanII G_rGCy'C

Cuts at:      0      59     203     277     458     585 
   Size:         59     144      74     181     127

  Fragments arranged by size: 

                 181     144     127      74      59
 
BbeI G_GCGC'C

Cuts at:      0     197     585 
   Size:        197     388
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     546     585 
   Size:        546      39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     202     299     517     585 
   Size:         55     147      97     218      68

  Fragments arranged by size: 

                 218     147      97      68      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     585 
   Size:         43     542
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     178     585 
   Size:        178     407
 
BsaHI Gr'CG_yC

Cuts at:      0      17     143     194     585 
   Size:         17     126      51     391

  Fragments arranged by size: 

                 391     126      51      17
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     538     568     585 
   Size:        538      30      17

  Fragments arranged by size: 

                 538      30      17
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     299     520     585 
   Size:         67     232     221      65

  Fragments arranged by size: 

                 232     221      67      65
 
BseYI C'CCAG_C

Cuts at:      0     292     585 
   Size:        292     293
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     585 
   Size:        319     266
 
BsiHKAI G_wGCw'C

Cuts at:      0      59     203     585 
   Size:         59     144     382

  Fragments arranged by size: 

                 382     144      59
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     203     277     458     585 
   Size:         59     144      74     181     127

  Fragments arranged by size: 

                 181     144     127      74      59
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     523     585 
   Size:        523      62
 
BsrBI CCG'CTC

Cuts at:      0     579     585 
   Size:        579       6
 
BssHII G'CGCG_C

Cuts at:      0       6       8      10     125     585 
   Size:          6       2       2     115     460

  Fragments arranged by size: 

                 460     115       6       2       2
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     277     585 
   Size:        277     308
 
BstYI r'GATC_y

Cuts at:      0     296     585 
   Size:        296     289
 
Bsu36I CC'TnA_GG

Cuts at:      0     541     585 
   Size:        541      44
 
BtgI C'CryG_G

Cuts at:      0     118     137     585 
   Size:        118      19     448

  Fragments arranged by size: 

                 448     118      19
 
BtsI GCAGTG_nn'

Cuts at:      0     449     585 
   Size:        449     136
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     166     585 
   Size:        166     419
 
EaeI y'GGCC_r

Cuts at:      0     242     260     585 
   Size:        242      18     325

  Fragments arranged by size: 

                 325     242      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     490     585 
   Size:        490      95
 
EcoICRI GAG'CTC

Cuts at:      0      57     201     585 
   Size:         57     144     384

  Fragments arranged by size: 

                 384     144      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55      94     202     299     471     517     585 
   Size:         55      39     108      97     172      46      68

  Fragments arranged by size: 

                 172     108      97      68      55      46      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     565     585 
   Size:        565      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0     303     417     537     570     585 
   Size:        303     114     120      33      15

  Fragments arranged by size: 

                 303     120     114      33      15
 
HaeII r_GCGC'y

Cuts at:      0     197     585 
   Size:        197     388
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     538     570     585 
   Size:        538      32      15

  Fragments arranged by size: 

                 538      32      15
 
KasI G'GCGC_C

Cuts at:      0     193     585 
   Size:        193     392
 
MspA1I CmG'CkG

Cuts at:      0      93     120     252     292     585 
   Size:         93      27     132      40     293

  Fragments arranged by size: 

                 293     132      93      40      27
 
NarI GG'CG_CC

Cuts at:      0     194     585 
   Size:        194     391
 
NcoI C'CATG_G

Cuts at:      0     137     585 
   Size:        137     448
 
NruI TCG'CGA

Cuts at:      0     370     585 
   Size:        370     215
 
PfoI T'CCnGG_A

Cuts at:      0      23     585 
   Size:         23     562
 
PpuMI rG'GwC_Cy

Cuts at:      0     417     570     585 
   Size:        417     153      15

  Fragments arranged by size: 

                 417     153      15
 
PstI C_TGCA'G

Cuts at:      0     236     257     585 
   Size:        236      21     328

  Fragments arranged by size: 

                 328     236      21
 
PvuII CAG'CTG

Cuts at:      0     252     292     585 
   Size:        252      40     293

  Fragments arranged by size: 

                 293     252      40
 
SacI G_AGCT'C

Cuts at:      0      59     203     585 
   Size:         59     144     382

  Fragments arranged by size: 

                 382     144      59
 
SacII CC_GC'GG

Cuts at:      0     121     585 
   Size:        121     464
 
SapI GCTCTTCn'nnn_

Cuts at:      0     490     585 
   Size:        490      95
 
SbfI CC_TGCA'GG

Cuts at:      0     236     585 
   Size:        236     349
 
SexAI A'CCwGG_T

Cuts at:      0     560     585 
   Size:        560      25
 
SfcI C'TryA_G

Cuts at:      0     232     253     585 
   Size:        232      21     332

  Fragments arranged by size: 

                 332     232      21
 
SfoI GGC'GCC

Cuts at:      0     195     585 
   Size:        195     390
 
SmlI C'TyrA_G

Cuts at:      0      58     585 
   Size:         58     527
 
StuI AGG'CCT

Cuts at:      0     332     585 
   Size:        332     253
 
StyI C'CwwG_G

Cuts at:      0     137     146     585 
   Size:        137       9     439

  Fragments arranged by size: 

                 439     137       9
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     144     506     585 
   Size:        144     362      79

  Fragments arranged by size: 

                 362     144      79
 
XmnI GAAnn'nnTTC

Cuts at:      0     484     585 
   Size:        484     101
 

 Enzymes that do cut:

     AclI     AcuI     AlfI    BamHI     BanI    BanII     BbeI     BmrI
     BpmI    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrBI   BssHII    BstXI    BstYI   Bsu36I
     BtgI     BtsI     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I     KasI   MspA1I     NarI     NcoI     NruI
     PfoI    PpuMI     PstI    PvuII     SacI    SacII     SapI     SbfI
    SexAI     SfcI     SfoI     SmlI     StuI     StyI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     EagI     EciI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NdeI   NgoMIV     NheI     NotI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SalI
    SanDI     ScaI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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