Restriction map for pF1KB6588
 (Linear) (Six-base) MAPSORT of: KIBB6588.seq  Check: 6685  from: 1  to: 1218



KIBB6588.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0     290     392    1218 
   Size:        290     102     826

  Fragments arranged by size: 

                 826     290     102
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293    1091    1218 
   Size:        293     798     127

  Fragments arranged by size: 

                 798     293     127
 
AflIII A'CryG_T

Cuts at:      0    1193    1218 
   Size:       1193      25
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1109    1141    1218 
   Size:       1109      32      77

  Fragments arranged by size: 

                1109      77      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     195     580    1069    1096    1218 
   Size:        195     385     489      27     122

  Fragments arranged by size: 

                 489     385     195     122      27
 
ApaLI G'TGCA_C

Cuts at:      0    1086    1218 
   Size:       1086     132
 
ApoI r'AATT_y

Cuts at:      0     280     664     946    1218 
   Size:        280     384     282     272

  Fragments arranged by size: 

                 384     282     280     272
 
AvaI C'yCGr_G

Cuts at:      0     599    1016    1218 
   Size:        599     417     202

  Fragments arranged by size: 

                 599     417     202
 
BanII G_rGCy'C

Cuts at:      0     211     521    1049    1218 
   Size:        211     310     528     169

  Fragments arranged by size: 

                 528     310     211     169
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     592    1218 
   Size:        592     626
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     139     173    1218 
   Size:        139      34    1045

  Fragments arranged by size: 

                1045     139      34
 
BlpI GC'TnA_GC

Cuts at:      0     542    1043    1218 
   Size:        542     501     175

  Fragments arranged by size: 

                 542     501     175
 
Bme1580I G_kGCm'C

Cuts at:      0    1090    1218 
   Size:       1090     128
 
BmgBI CAC'GTC

Cuts at:      0     294    1218 
   Size:        294     924
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     541     590    1218 
   Size:        541      49     628

  Fragments arranged by size: 

                 628     541      49
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     225    1218 
   Size:        225     993
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52      76     557     796     809     841    1218 
   Size:         52      24     481     239      13      32     377

  Fragments arranged by size: 

                 481     377     239      52      32      24      13
 
Bpu10I CC'TnA_GC

Cuts at:      0     515    1218 
   Size:        515     703
 
BsaBI GATnn'nnATC

Cuts at:      0     895    1218 
   Size:        895     323
 
BsaWI w'CCGG_w

Cuts at:      0     125    1218 
   Size:        125    1093
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49     196     756     979    1218 
   Size:         49     147     560     223     239

  Fragments arranged by size: 

                 560     239     223     147      49
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     826    1218 
   Size:        352     474     392

  Fragments arranged by size: 

                 474     392     352
 
BsiHKAI G_wGCw'C

Cuts at:      0     521    1049    1090    1218 
   Size:        521     528      41     128

  Fragments arranged by size: 

                 528     521     128      41
 
Bsp1286I G_dGCh'C

Cuts at:      0     211     521    1049    1090    1218 
   Size:        211     310     528      41     128

  Fragments arranged by size: 

                 528     310     211     128      41
 
BspEI T'CCGG_A

Cuts at:      0     125    1218 
   Size:        125    1093
 
BsrDI GCAATG_nn'

Cuts at:      0     536    1218 
   Size:        536     682
 
BstBI TT'CG_AA

Cuts at:      0     524    1218 
   Size:        524     694
 
BstYI r'GATC_y

Cuts at:      0     374    1218 
   Size:        374     844
 
BtgI C'CryG_G

Cuts at:      0     236    1218 
   Size:        236     982
 
BtsI GCAGTG_nn'

Cuts at:      0     910    1218 
   Size:        910     308
 
EarI CTCTTCn'nnn_

Cuts at:      0     526    1218 
   Size:        526     692
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      49    1218 
   Size:         49    1169
 
EcoICRI GAG'CTC

Cuts at:      0     519    1047    1218 
   Size:        519     528     171

  Fragments arranged by size: 

                 528     519     171
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52      76     293     557     796     809     841    1091 
   Size:         52      24     217     264     239      13      32     250

Cuts at:   1091    1218 
   Size:        127

  Fragments arranged by size: 

                 264     250     239     217     127      52      32      24
                  13
 
EcoRI G'AATT_C

Cuts at:      0     946    1218 
   Size:        946     272
 
EcoRV GAT'ATC

Cuts at:      0     897    1218 
   Size:        897     321
 
HaeII r_GCGC'y

Cuts at:      0       7    1218 
   Size:          7    1211
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     981    1013    1115    1147    1218 
   Size:        981      32     102      32      71

  Fragments arranged by size: 

                 981     102      71      32      32
 
MfeI C'AATT_G

Cuts at:      0     330    1218 
   Size:        330     888
 
MslI CAynn'nnrTG

Cuts at:      0     739    1218 
   Size:        739     479
 
MspA1I CmG'CkG

Cuts at:      0      53      96     192    1218 
   Size:         53      43      96    1026

  Fragments arranged by size: 

                1026      96      53      43
 
NcoI C'CATG_G

Cuts at:      0     236    1218 
   Size:        236     982
 
NspI r_CATG'y

Cuts at:      0     724    1104    1197    1218 
   Size:        724     380      93      21

  Fragments arranged by size: 

                 724     380      93      21
 
PasI CC'CwG_GG

Cuts at:      0     922    1218 
   Size:        922     296
 
PciI A'CATG_T

Cuts at:      0    1193    1218 
   Size:       1193      25
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     580    1218 
   Size:        580     638
 
PfoI T'CCnGG_A

Cuts at:      0     899    1218 
   Size:        899     319
 
PstI C_TGCA'G

Cuts at:      0     137     200    1218 
   Size:        137      63    1018

  Fragments arranged by size: 

                1018     137      63
 
PvuII CAG'CTG

Cuts at:      0      96     192    1218 
   Size:         96      96    1026

  Fragments arranged by size: 

                1026      96      96
 
SacI G_AGCT'C

Cuts at:      0     521    1049    1218 
   Size:        521     528     169

  Fragments arranged by size: 

                 528     521     169
 
SapI GCTCTTCn'nnn_

Cuts at:      0     526    1218 
   Size:        526     692
 
SfcI C'TryA_G

Cuts at:      0     133     187     196    1218 
   Size:        133      54       9    1022

  Fragments arranged by size: 

                1022     133      54       9
 
SmaI CCC'GGG

Cuts at:      0     601    1018    1218 
   Size:        601     417     200

  Fragments arranged by size: 

                 601     417     200
 
SrfI GCCC'GGGC

Cuts at:      0     601    1218 
   Size:        601     617
 
SspI AAT'ATT

Cuts at:      0      67    1218 
   Size:         67    1151
 
StyI C'CwwG_G

Cuts at:      0     236     548    1163    1218 
   Size:        236     312     615      55

  Fragments arranged by size: 

                 615     312     236      55
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     342    1218 
   Size:        342     876
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     336    1218 
   Size:        336     882
 
XmaI C'CCGG_G

Cuts at:      0     599    1016    1218 
   Size:        599     417     202

  Fragments arranged by size: 

                 599     417     202
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AloI    AlwNI    ApaLI     ApoI     AvaI
    BanII     BbsI     BcgI     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BsaBI    BsaWI    BseRI     BsgI  BsiHKAI Bsp1286I
    BspEI    BsrDI    BstBI    BstYI     BtgI     BtsI     EarI     EciI
  EcoICRI  Eco57MI    EcoRI    EcoRV    HaeII    Hin4I     MfeI     MslI
   MspA1I     NcoI     NspI     PasI     PciI    PflMI     PfoI     PstI
    PvuII     SacI     SapI     SfcI     SmaI     SrfI     SspI     StyI
    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BmtI    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
    EcoNI EcoO109I     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmnI
     ZraI
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