Restriction map for pF1KB6515
 (Linear) (Six-base) MAPSORT of: KIBB6515.seq  Check: 8481  from: 1  to: 1080



KIBB6515.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     625    1080 
   Size:        418     207     455

  Fragments arranged by size: 

                 455     418     207
 
AflII C'TTAA_G

Cuts at:      0     996    1080 
   Size:        996      84
 
AflIII A'CryG_T

Cuts at:      0     442    1080 
   Size:        442     638
 
AleI CACnn'nnGTG

Cuts at:      0     452    1080 
   Size:        452     628
 
AlwNI CAG_nnn'CTG

Cuts at:      0     167    1080 
   Size:        167     913
 
AvaI C'yCGr_G

Cuts at:      0     836    1080 
   Size:        836     244
 
BanI G'GyrC_C

Cuts at:      0     257    1080 
   Size:        257     823
 
BanII G_rGCy'C

Cuts at:      0     922    1080 
   Size:        922     158
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      27     406     721    1080 
   Size:         27     379     315     359

  Fragments arranged by size: 

                 379     359     315      27
 
BclI T'GATC_A

Cuts at:      0     488    1080 
   Size:        488     592
 
BglII A'GATC_T

Cuts at:      0     690    1080 
   Size:        690     390
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     617    1080 
   Size:        617     463
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     518     962    1080 
   Size:        370     148     444     118

  Fragments arranged by size: 

                 444     370     148     118
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      87     117     909     939    1080 
   Size:         87      30     792      30     141

  Fragments arranged by size: 

                 792     141      87      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      92      95     545     548     551     575     930    1080 
   Size:         92       3     450       3       3      24     355     150

  Fragments arranged by size: 

                 450     355     150      92      24       3       3       3
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     789    1080 
   Size:        789     291
 
Bsp1286I G_dGCh'C

Cuts at:      0     922    1080 
   Size:        922     158
 
BstYI r'GATC_y

Cuts at:      0     690     879    1080 
   Size:        690     189     201

  Fragments arranged by size: 

                 690     201     189
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     737    1080 
   Size:        737     343
 
BtsI GCAGTG_nn'

Cuts at:      0     526    1080 
   Size:        526     554
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     418     518     625     962    1080 
   Size:        370      48     100     107     337     118

  Fragments arranged by size: 

                 370     337     118     107     100      48
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     532    1080 
   Size:        532     548
 
HindIII A'AGCT_T

Cuts at:      0     742    1080 
   Size:        742     338
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1063    1080 
   Size:       1063      17
 
MslI CAynn'nnrTG

Cuts at:      0     452    1080 
   Size:        452     628
 
MspA1I CmG'CkG

Cuts at:      0     254    1014    1080 
   Size:        254     760      66

  Fragments arranged by size: 

                 760     254      66
 
NspI r_CATG'y

Cuts at:      0     446     802    1080 
   Size:        446     356     278

  Fragments arranged by size: 

                 446     356     278
 
PciI A'CATG_T

Cuts at:      0     442    1080 
   Size:        442     638
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     433    1080 
   Size:        433     647
 
PfoI T'CCnGG_A

Cuts at:      0     181     386     861    1080 
   Size:        181     205     475     219

  Fragments arranged by size: 

                 475     219     205     181
 
PspXI vC'TCGA_Gb

Cuts at:      0     836    1080 
   Size:        836     244
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     921     953    1080 
   Size:        921      32     127

  Fragments arranged by size: 

                 921     127      32
 
PvuII CAG'CTG

Cuts at:      0     254    1014    1080 
   Size:        254     760      66

  Fragments arranged by size: 

                 760     254      66
 
SmlI C'TyrA_G

Cuts at:      0     836     996    1080 
   Size:        836     160      84

  Fragments arranged by size: 

                 836     160      84
 
SpeI A'CTAG_T

Cuts at:      0     774    1080 
   Size:        774     306
 
StuI AGG'CCT

Cuts at:      0     994    1080 
   Size:        994      86
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      67      99     291     323     909     941    1080 
   Size:         67      32     192      32     586      32     139

  Fragments arranged by size: 

                 586     192     139      67      32      32      32
 
XhoI C'TCGA_G

Cuts at:      0     836    1080 
   Size:        836     244
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AleI    AlwNI     AvaI     BanI    BanII
     BbsI     BclI    BglII     BmrI     BpmI    BsaXI    BseRI    BsmBI
 Bsp1286I    BstYI    BtgZI     BtsI  Eco57MI     FalI  HindIII     MmeI
     MslI   MspA1I     NspI     PciI    PflMI     PfoI    PspXI     PsrI
    PvuII     SmlI     SpeI     StuI     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SphI     SrfI     SspI     StyI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI
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