Restriction map for pF1KB6135
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AgeI        Alw44I      AlwNI       
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BanI        BbeI        BbvCI       BciVI       BclI        
BfaI        BglI        BlnI        BlpI        Bpu1102I    
BsaI        BsiWI       BsmBI       BsmI        Bsp1407I    
BspHI       BsrDI       BsrGI       BssHII      Bst1107I    
BstZ17I     CelII       CpoI        DraIII      DrdI        
EcoNI       EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MfeI        MluNI       
MscI        MunI        NarI        NcoI        NheI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshBI       PvuI        RcaI        RsrII       
SacII       SalI        SapI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI          583  GTAGAC               GT^[AC][GT]AC
AccIII       1424  TCCGGA               T^CCGGA
AclI         1141  AACGTT               AA^CGTT
AcsI          827  AAATTT               [GA]^AATT[CT]
AflII         691  CTTAAG               C^TTAAG
AocI         1045  CCTGAGG              CC^T[ACGT]AGG
ApaI         1206  GGGCCC               GGGCC^C
ApoI          827  AAATTT               [GA]^AATT[CT]
AvaI          288  CCCGAG               C^[CT]CG[GA]G
BamHI        1427  GGATCC               G^GATCC
BbrPI         904  CACGTG               CAC^GTG
BbsI          328  GAAGAC               GAAGAC(2/6)
BcgI         1686  CGATGAGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          691  CTTAAG               C^TTAAG
BglII        1076  AGATCT               A^GATCT
BpuAI         328  GAAGAC               GAAGAC(2/6)
BsaAI         904  CACGTG               [CT]AC^GT[GA]
BsaBI        1077  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1424  TCCGGA               [AT]^CCGG[AT]
BseAI        1424  TCCGGA               T^CCGGA
BsoBI         288  CCCGAG               C^[CT]CG[GA]G
BspDI         703  ATCGAT               AT^CGAT
BspEI        1424  TCCGGA               T^CCGGA
BspLU11I      137  ACATGT               A^CATGT
BspMI         535  ACCTGC               ACCTGC(4/8)
BssSI        1102  CACGAG               CACGAG(-5/-1)
BstBI        1723  TTCGAA               TT^CGAA
BstEII        193  GGTGACC              G^GT[ACGT]ACC
BstPI         193  GGTGACC              G^GT[ACGT]ACC
BstXI         836  CCATGAAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1045  CCTGAGG              CC^T[ACGT]AGG
ClaI          703  ATCGAT               AT^CGAT
DsaI          516  CCACGG               C^C[GA][CT]GG
Eco81I       1045  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       193  GGTGACC              G^GT[ACGT]ACC
EcoT14I       791  CCAAGG               C^C[AT][AT]GG
HincII        997  GTCAAC               GT[CT]^[GA]AC
HindII        997  GTCAAC               GT[CT]^[GA]AC
MamI         1077  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1544  ACGCGT               A^CGCGT
MroI         1424  TCCGGA               T^CCGGA
NaeI         1508  GCCGGC               GCC^GGC
NdeI          547  CATATG               CA^TATG
NgoMIV       1508  GCCGGC               G^CCGGC
NotI           38  GCGGCCGC             GC^GGCCGC
NruI          461  TCGCGA               TCG^CGA
NspV         1723  TTCGAA               TT^CGAA
PmaCI         904  CACGTG               CAC^GTG
PmlI          904  CACGTG               CAC^GTG
Psp1406I     1141  AACGTT               AA^CGTT
PvuII        1672  CAGCTG               CAG^CTG
SacI          115  GAGCTC               GAGCT^C
SbfI          536  CCTGCAGG             CCTGCA^GG
SfuI         1723  TTCGAA               TT^CGAA
Sse8387I      536  CCTGCAGG             CCTGCA^GG
StyI          791  CCAAGG               C^C[AT][AT]GG
XcmI          791  CCAAGGTTCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcyI           94  GACGCC               G[GA]^CG[CT]C
              601  GACGCC              
AhdI          963  GACGACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1531  GACATCAAGTC         
Aor51HI       407  AGCGCT               AGC^GCT
              509  AGCGCT              
AspEI         963  GACGACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1531  GACATCAAGTC         
BsaHI          94  GACGCC               G[GA]^CG[CT]C
              601  GACGCC              
BsiEI          39  CGGCCG               CG[GA][CT]^CG
             1070  CGGCCG              
BsmAI         134  GAGAC                GTCTC(1/5)
              622  GAGAC               
BsrBI         163  CCGCTC               CCGCTC(-3/-3)
               33  GAGCGG              
BsrFI          13  ACCGGC               [GA]^CCGG[CT]
             1508  GCCGGC              
BstAPI        482  GCATCAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1226  GCACTTTCTGC         
BstYI        1076  AGATCT               [GA]^GATC[CT]
             1427  GGATCC              
Cfr10I         13  ACCGGC               [GA]^CCGG[CT]
             1508  GCCGGC              
DraI         1464  TTTAAA               TTT^AAA
             1476  TTTAAA              
EagI           39  CGGCCG               C^GGCCG
             1070  CGGCCG              
Eam1105I      963  GACGACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1531  GACATCAAGTC         
EarI          394  CTCTTC               CTCTTC(1/4)
              229  GAAGAG              
EclXI          39  CGGCCG               C^GGCCG
             1070  CGGCCG              
Eco47III      407  AGCGCT               AGC^GCT
              509  AGCGCT              
Eco52I         39  CGGCCG               C^GGCCG
             1070  CGGCCG              
Hin1I          94  GACGCC               G[GA]^CG[CT]C
              601  GACGCC              
Ksp632I       394  CTCTTC               CTCTTC(1/4)
              229  GAAGAG              
MaeIII        194  GTGAC                ^GT[ACGT]AC
             1214  GTGAC               
MflI         1076  AGATCT               [GA]^GATC[CT]
             1427  GGATCC              
MslI           84  CACCAGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              837  CATGAAAGTG          
MspA1I        529  CCGCTG               C[AC]G^C[GT]G
             1672  CAGCTG              
PflMI         356  CCAGAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1513  CCACAGACTGG         
PshAI         136  GACATGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1624  GACTACGGTC          
PstI          537  CTGCAG               CTGCA^G
              745  CTGCAG              
StuI          267  AGGCCT               AGG^CCT
             1200  AGGCCT              
TfiI          553  GATTC                G^A[AT]TC
              719  GAATC               
Tsp45I        194  GTGAC                ^GT[GC]AC
             1214  GTGAC               
TspRI         253  CAGTG                CA[GC]TG[ACGT][ACGT]^
              436  CAGTG               
Van91I        356  CCAGAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1513  CCACAGACTGG         
XhoII        1076  AGATCT               [GA]^GATC[CT]
             1427  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AspHI       AvaII       BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MboI        
MboII       MnlI        MseI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        TaqI        Tru9I       TseI        Tsp509I     
TthHB8I     
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