Restriction map for pF1KB7860
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwNI       AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BfrI        BglI        BglII       BlnI        
BsaBI       BsaHI       BsiEI       BsiWI       BsmBI       
BspDI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        MvnI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
XhoI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Alw44I       1762  GTGCAC               G^TGCAC
AlwI          775  GATCC                GGATC(4/5)
ApaLI        1762  GTGCAC               G^TGCAC
Asp700I       615  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI           44  CCCGGG               C^[CT]CG[GA]G
BalI         1389  TGGCCA               TGG^CCA
BanI          261  GGCACC               G^G[CT][GA]CC
BanII          64  GGGCTC               G[GA]GC[CT]^C
BbvCI         388  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1239  GGATAC               GTATCC(6/5)
BfaI          446  CTAG                 C^TAG
BlpI          547  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      547  GCTGAGC              GC^T[ACGT]AGC
BsaAI          13  TACGTG               [CT]AC^GT[GA]
BsaI         1110  GAGACC               GGTCTC(1/5)
BsgI         1554  GTGCAG               GTGCAG(16/14)
BsoBI          44  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      203  TGTACA               T^GTACA
BspMI        1172  ACCTGC               ACCTGC(4/8)
BsrDI         563  CATTGC               GCAATG(2/0)
BsrGI         203  TGTACA               T^GTACA
BstAPI        633  GCAGGCTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         774  AGATCC               [GA]^GATC[CT]
CelII         547  GCTGAGC              GC^T[ACGT]AGC
EaeI         1389  TGGCCA               [CT]^GGCC[GA]
EcoNI        1226  CCTTTAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII       787  AAGCTT               A^AGCTT
MaeI          446  CTAG                 C^TAG
MflI          774  AGATCC               [GA]^GATC[CT]
MluNI        1389  TGGCCA               TGG^CCA
MscI         1389  TGGCCA               TGG^CCA
NcoI         1779  CCATGG               C^CATGG
NdeI          919  CATATG               CA^TATG
NspI            8  GCATGT               [GA]CATG^[CT]
PflMI        1381  CCATATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1686  GACTC                GAGTC(4/5)
PvuII         708  CAGCTG               CAG^CTG
SapI          726  GAAGAGC              GCTCTTC(1/4)
SfcI          835  CTACAG               C^T[GA][CT]AG
SmaI           44  CCCGGG               CCC^GGG
SspBI         203  TGTACA               T^GTACA
Tsp45I        377  GTGAC                ^GT[GC]AC
Van91I       1381  CCATATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          445  TCTAGA               T^CTAGA
XcmI         1697  CCAATCGTCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         774  AGATCC               [GA]^GATC[CT]
XmaCI          44  CCCGGG               C^CCGGG
XmaI           44  CCCGGG               C^CCGGG
XmnI          615  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccIII       1145  TCCGGA               T^CCGGA
             1430  TCCGGA              
AciI           78  CCGC                 CCGC(-3/-1)
              463  GCGG                
AvaII         106  GGACC                G^G[AT]CC
             1148  GGACC               
BclI          923  TGATCA               T^GATCA
              987  TGATCA              
BsaWI        1145  TCCGGA               [AT]^CCGG[AT]
             1430  TCCGGA              
BseAI        1145  TCCGGA               T^CCGGA
             1430  TCCGGA              
BsmFI        1451  GTCCC                GGGAC(10/14)
             1703  GTCCC               
BsmI          128  GAATGC               GAATGC(1/-1)
             1714  GAATGC              
BspEI        1145  TCCGGA               T^CCGGA
             1430  TCCGGA              
DsaI          226  CCACGG               C^C[GA][CT]GG
             1779  CCATGG              
EarI          123  GAAGAG               CTCTTC(1/4)
              726  GAAGAG              
EcoT14I       480  CCTTGG               C^C[AT][AT]GG
             1779  CCATGG              
FauI           77  CCCGC                CCCGC(4/6)
              463  GCGGG               
FbaI          923  TGATCA               T^GATCA
              987  TGATCA              
HgaI         1602  GACGC                GACGC(5/10)
             1449  GCGTC               
Ksp632I       123  GAAGAG               CTCTTC(1/4)
              726  GAAGAG              
MaeII          14  ACGT                 A^CGT
              915  ACGT                
MaeIII        377  GTGAC                ^GT[ACGT]AC
             1318  GTTAC               
MroI         1145  TCCGGA               T^CCGGA
             1430  TCCGGA              
MspA1I        461  CAGCGG               C[AC]G^C[GT]G
              708  CAGCTG              
StyI          480  CCTTGG               C^C[AT][AT]GG
             1779  CCATGG              
TaqI          520  TCGA                 T^CGA
             1674  TCGA                
TthHB8I       520  TCGA                 T^CGA
             1674  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        AspHI       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BspHI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MboI        MboII       MnlI        MseI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StuI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
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