Restriction map for pF1KB6411
 (Linear) (Six-base) MAPSORT of: KIBB6411.seq  Check: 4319  from: 1  to: 921



KIBB6411.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AatII G_ACGT'C

Cuts at:      0     215     921 
   Size:        215     706
 
AclI AA'CG_TT

Cuts at:      0     277     921 
   Size:        277     644
 
AflII C'TTAA_G

Cuts at:      0     298     921 
   Size:        298     623
 
AlwNI CAG_nnn'CTG

Cuts at:      0     519     921 
   Size:        519     402
 
ApaI G_GGCC'C

Cuts at:      0     572     921 
   Size:        572     349
 
ApaLI G'TGCA_C

Cuts at:      0     487     921 
   Size:        487     434
 
AvaI C'yCGr_G

Cuts at:      0     629     921 
   Size:        629     292
 
BanI G'GyrC_C

Cuts at:      0     555     774     921 
   Size:        555     219     147

  Fragments arranged by size: 

                 555     219     147
 
BanII G_rGCy'C

Cuts at:      0      56     572     628     921 
   Size:         56     516      56     293

  Fragments arranged by size: 

                 516     293      56      56
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     608     921 
   Size:        608     313
 
BbvCI CC'TCA_GC

Cuts at:      0     448     921 
   Size:        448     473
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      25      59     921 
   Size:         25      34     862

  Fragments arranged by size: 

                 862      34      25
 
BglII A'GATC_T

Cuts at:      0     101     434     476     921 
   Size:        101     333      42     445

  Fragments arranged by size: 

                 445     333     101      42
 
Bme1580I G_kGCm'C

Cuts at:      0     491     572     711     921 
   Size:        491      81     139     210

  Fragments arranged by size: 

                 491     210     139      81
 
BmgBI CAC'GTC

Cuts at:      0     660     921 
   Size:        660     261
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     663     921 
   Size:        663     258
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820     921 
   Size:        820     101
 
Bpu10I CC'TnA_GC

Cuts at:      0      89     448     921 
   Size:         89     359     473

  Fragments arranged by size: 

                 473     359      89
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     214     921 
   Size:        214     707
 
BsaHI Gr'CG_yC

Cuts at:      0     212     921 
   Size:        212     709
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     574     647     921 
   Size:        127     447      73     274

  Fragments arranged by size: 

                 447     274     127      73
 
BseYI C'CCAG_C

Cuts at:      0     614     921 
   Size:        614     307
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     411     921 
   Size:        145     266     510

  Fragments arranged by size: 

                 510     266     145
 
BsiEI CG_ry'CG

Cuts at:      0     543     921 
   Size:        543     378
 
BsiHKAI G_wGCw'C

Cuts at:      0      56     491     761     921 
   Size:         56     435     270     160

  Fragments arranged by size: 

                 435     270     160      56
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     745     921 
   Size:        745     176
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     491     572     628     711     761     921 
   Size:         56     435      81      56      83      50     160

  Fragments arranged by size: 

                 435     160      83      81      56      56      50
 
BspHI T'CATG_A

Cuts at:      0     561     921 
   Size:        561     360
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     354     921 
   Size:        354     567
 
BsrFI r'CCGG_y

Cuts at:      0      23     637     921 
   Size:         23     614     284

  Fragments arranged by size: 

                 614     284      23
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     555     921 
   Size:        555     366
 
BstYI r'GATC_y

Cuts at:      0     101     434     476     921 
   Size:        101     333      42     445

  Fragments arranged by size: 

                 445     333     101      42
 
BtgI C'CryG_G

Cuts at:      0     165     255     902     921 
   Size:        165      90     647      19

  Fragments arranged by size: 

                 647     165      90      19
 
BtsI GCAGTG_nn'

Cuts at:      0     660     921 
   Size:        660     261
 
ClaI AT'CG_AT

Cuts at:      0     467     921 
   Size:        467     454
 
DraIII CAC_nnn'GTG

Cuts at:      0     152     767     921 
   Size:        152     615     154

  Fragments arranged by size: 

                 615     154     152
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     142     921 
   Size:        142     779
 
EaeI y'GGCC_r

Cuts at:      0     905     921 
   Size:        905      16
 
EarI CTCTTCn'nnn_

Cuts at:      0     238     921 
   Size:        238     683
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     921 
   Size:         19     902
 
EcoICRI GAG'CTC

Cuts at:      0      54     921 
   Size:         54     867
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820     921 
   Size:        820     101
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78     495     921 
   Size:         78     417     426

  Fragments arranged by size: 

                 426     417      78
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     759     921 
   Size:        759     162
 
FspI TGC'GCA

Cuts at:      0     394     921 
   Size:        394     527
 
HaeII r_GCGC'y

Cuts at:      0     423     921 
   Size:        423     498
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     507     539     921 
   Size:        507      32     382

  Fragments arranged by size: 

                 507     382      32
 
MslI CAynn'nnrTG

Cuts at:      0     694     746     921 
   Size:        694      52     175

  Fragments arranged by size: 

                 694     175      52
 
MspA1I CmG'CkG

Cuts at:      0      39     794     921 
   Size:         39     755     127

  Fragments arranged by size: 

                 755     127      39
 
NaeI GCC'GGC

Cuts at:      0     639     921 
   Size:        639     282
 
NcoI C'CATG_G

Cuts at:      0     165     921 
   Size:        165     756
 
NgoMIV G'CCGG_C

Cuts at:      0     637     921 
   Size:        637     284
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     519     614     717     921 
   Size:        519      95     103     204

  Fragments arranged by size: 

                 519     204     103      95
 
PspOMI G'GGCC_C

Cuts at:      0     568     921 
   Size:        568     353
 
PvuII CAG'CTG

Cuts at:      0      39     921 
   Size:         39     882
 
SacI G_AGCT'C

Cuts at:      0      56     921 
   Size:         56     865
 
SfcI C'TryA_G

Cuts at:      0     264     921 
   Size:        264     657
 
SmlI C'TyrA_G

Cuts at:      0     298     921 
   Size:        298     623
 
StyI C'CwwG_G

Cuts at:      0     165     921 
   Size:        165     756
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     557     792     921 
   Size:        557     235     129

  Fragments arranged by size: 

                 557     235     129
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     869     901     921 
   Size:        869      32      20

  Fragments arranged by size: 

                 869      32      20
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     102     516     819     900     921 
   Size:        102     414     303      81      21

  Fragments arranged by size: 

                 414     303     102      81      21
 
XmnI GAAnn'nnTTC

Cuts at:      0     844     921 
   Size:        844      77
 
ZraI GAC'GTC

Cuts at:      0     213     921 
   Size:        213     708
 

 Enzymes that do cut:

    AatII     AclI    AflII    AlwNI     ApaI    ApaLI     AvaI     BanI
    BanII     BbsI    BbvCI     BcgI    BglII Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I     BsaI    BsaHI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrFI   BstAPI    BstYI
     BtgI     BtsI     ClaI   DraIII     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     FalI     FspI    HaeII    Hin4I     MslI
   MspA1I     NaeI     NcoI   NgoMIV    PflMI   PspOMI    PvuII     SacI
     SfcI     SmlI     StyI    TaqII     TstI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BciVI     BclI    BfrBI     BglI     BlpI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BsiWI
     BsmI    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI    CspCI     DraI     EagI
    EcoNI    EcoRI    EcoRV     FseI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmaI
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