Restriction map for pF1KB7775
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        AspEI       
AspHI       AvaI        AvaII       AviII       AvrII       
BanII       BbrPI       BbvCI       BcgI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspEI       BspLU11I    BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRV       EcoT22I     FauI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KspI        MfeI        MluI        MroI        
MslI        MspA1I      MunI        MvnI        NaeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfaNI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    StuI        
SwaI        Tsp45I      Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

Acc65I        557  GGTACC               G^GTACC
Asp718I       557  GGTACC               G^GTACC
AspI          994  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          837  TGGCCA               TGG^CCA
BamHI        1203  GGATCC               G^GATCC
BbeI           26  GGCGCC               GGCGC^C
BbsI          925  GTCTTC               GAAGAC(2/6)
BciVI        1075  GGATAC               GTATCC(6/5)
BclI          350  TGATCA               T^GATCA
BcnI          170  CCCGG                CC^[GC]GG
BpmI          389  CTGGAG               CTGGAG(16/14)
BpuAI         925  GTCTTC               GAAGAC(2/6)
BsaBI         856  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I      658  TGTACA               T^GTACA
BspDI        1273  ATCGAT               AT^CGAT
BspHI         491  TCATGA               T^CATGA
BspMI        1026  ACCTGC               ACCTGC(4/8)
BsrGI         658  TGTACA               T^GTACA
BstNI         945  CCAGG                CC^[AT]GG
CfoI           27  GCGC                 GCG^C
Cfr13I       1112  GGGCC                G^G[ACGT]CC
ClaI         1273  ATCGAT               AT^CGAT
DsaI         1137  CCATGG               C^C[GA][CT]GG
EaeI          837  TGGCCA               [CT]^GGCC[GA]
EarI         1220  CTCTTC               CTCTTC(1/4)
EcoRI         151  GAATTC               G^AATTC
EcoRII        945  CCAGG                ^CC[AT]GG
FbaI          350  TGATCA               T^GATCA
HaeII          26  GGCGCC               [GA]GCGC^[CT]
HapII         171  CCGG                 C^CGG
HgaI           36  GACGC                GACGC(5/10)
HhaI           27  GCGC                 GCG^C
HinP1I         27  GCGC                 G^CGC
HpaII         171  CCGG                 C^CGG
HphI          379  GGTGA                GGTGA(8/7)
KasI           26  GGCGCC               G^GCGCC
KpnI          557  GGTACC               GGTAC^C
Ksp632I      1220  CTCTTC               CTCTTC(1/4)
MaeII         607  ACGT                 A^CGT
MaeIII       1258  GTAAC                ^GT[ACGT]AC
MamI          856  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         837  TGGCCA               TGG^CCA
MscI          837  TGGCCA               TGG^CCA
MspI          171  CCGG                 C^CGG
MvaI          945  CCAGG                CC^[AT]GG
NarI           26  GGCGCC               GG^CGCC
NciI          170  CCCGG                CC^[GC]GG
NcoI         1137  CCATGG               C^CATGG
NdeI          452  CATATG               CA^TATG
PflFI         994  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         918  GACCAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          491  TCATGA               T^CATGA
Sau96I       1112  GGGCC                G^G[ACGT]CC
ScaI          842  AGTACT               AGT^ACT
SfoI           26  GGCGCC               GGC^GCC
SspBI         658  TGTACA               T^GTACA
SspI          110  AATATT               AAT^ATT
TaqI         1274  TCGA                 T^CGA
TspRI        1329  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       994  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1274  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI           26  GGCGCC               G[GA]^CG[CT]C
               36  GACGCC              
BanI           26  GGCGCC               G^G[CT][GA]CC
              557  GGTACC              
BfaI           31  CTAG                 C^TAG
              745  CTAG                
BsaHI          26  GGCGCC               G[GA]^CG[CT]C
               36  GACGCC              
BsiYI         164  CCATTCCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              328  CCTATGATTGG         
BslI          164  CCATTCCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              328  CCTATGATTGG         
BsmAI        1250  GAGAC                GTCTC(1/5)
              953  GTCTC               
BsrI            5  ACTGG                ACTGG(1/-1)
              840  CCAGT               
BssKI         170  CCCGG                ^CC[ACGT]GG
              945  CCAGG               
BstF5I        768  GGATG                GGATG(2/0)
             1173  GGATG               
EcoT14I       782  CCAAGG               C^C[AT][AT]GG
             1137  CCATGG              
FokI          768  GGATG                GGATG(9/13)
             1173  GGATG               
HaeIII        838  GGCC                 GG^CC
             1113  GGCC                
Hin1I          26  GGCGCC               G[GA]^CG[CT]C
               36  GACGCC              
MaeI           31  CTAG                 C^TAG
              745  CTAG                
MwoI          814  GCTCCTTCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1312  GCTCTTGTTGC         
NspI          733  GCATGC               [GA]CATG^[CT]
              872  GCATGC              
PleI          527  GACTC                GAGTC(4/5)
              789  GAGTC               
PstI         1028  CTGCAG               CTGCA^G
             1340  CTGCAG              
ScrFI         170  CCCGG                CC^[ACGT]GG
              945  CCAGG               
SphI          733  GCATGC               GCATG^C
              872  GCATGC              
StyI          782  CCAAGG               C^C[AT][AT]GG
             1137  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     BbvI        BsaJI       BstYI       Cac8I       
DdeI        DpnII       Fnu4HI      HinfI       ItaI        
MboI        MboII       MflI        MnlI        MseI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
SfcI        TfiI        Tru9I       TseI        Tsp509I     
XhoII       XmnI        
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