Restriction map for
pF1KB7775
(Circular) (Six-base) MAPSORT of: pF1KB7775.seq Check: 6832 from: 1 to: 4472
pF1KB7775 4472 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1510 1510
Size: 4472
Acc65I G'GTAC_C
Cuts at: 665 1488 665
Size: 823 3649
AclI AA'CG_TT
Cuts at: 4312 4312
Size: 4472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 1315 2297 2729 3476 3744 323
Size: 992 982 432 747 268 1051
Fragments arranged by size:
1051 992 982 747 432 268
AflIII A'CryG_T
Cuts at: 1824 2928 3905 1824
Size: 1104 977 2391
Fragments arranged by size:
2391 1104 977
AgeI A'CCGG_T
Cuts at: 2860 2860
Size: 4472
AlwNI CAG_nnn'CTG
Cuts at: 1777 3344 1777
Size: 1567 2905
ApaLI G'TGCA_C
Cuts at: 3242 3242
Size: 4472
ApoI r'AATT_y
Cuts at: 259 569 824 1476 3825 4081 259
Size: 310 255 652 2349 256 650
Fragments arranged by size:
2349 652 650 310 256 255
AseI AT'TA_AT
Cuts at: 20 20
Size: 4472
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
AvaI C'yCGr_G
Cuts at: 1492 1492
Size: 4472
BamHI G'GATC_C
Cuts at: 1311 1497 1311
Size: 186 4286
BanI G'GyrC_C
Cuts at: 134 665 1488 1902 2150 2185 134
Size: 531 823 414 248 35 2421
Fragments arranged by size:
2421 823 531 414 248 35
BanII G_rGCy'C
Cuts at: 1486 2516 1486
Size: 1030 3442
BbeI G_GCGC'C
Cuts at: 138 1906 2154 138
Size: 1768 248 2456
Fragments arranged by size:
2456 1768 248
BbsI GAAGACnn'nnnn_
Cuts at: 1026 1026
Size: 4472
BciVI GTATCCnnnnn_n'
Cuts at: 1177 2370 3131 4472 1177
Size: 1193 761 1341 1177
Fragments arranged by size:
1341 1193 1177 761
BclI T'GATC_A
Cuts at: 458 1992 458
Size: 1534 2938
BglII A'GATC_T
Cuts at: 1987 4024 1987
Size: 2037 2435
BlpI GC'TnA_GC
Cuts at: 1584 1584
Size: 4472
Bme1580I G_kGCm'C
Cuts at: 2097 2190 3246 2097
Size: 93 1056 3323
Fragments arranged by size:
3323 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1684 1765 2100 4244 1684
Size: 81 335 2144 1912
Fragments arranged by size:
2144 1912 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 4472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 518
Size: 4472
Bpu10I CC'TnA_GC
Cuts at: 871 871
Size: 4472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1648 2527 3019 3317 1648
Size: 879 492 298 2803
Fragments arranged by size:
2803 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4176 4176
Size: 4472
BsaAI yAC'GTr
Cuts at: 1825 1825
Size: 4472
BsaBI GATnn'nnATC
Cuts at: 6 968 1986 4023 6
Size: 962 1018 2037 455
Fragments arranged by size:
2037 1018 962 455
BsaHI Gr'CG_yC
Cuts at: 135 145 1903 2151 2853 4349 135
Size: 10 1758 248 702 1496 258
Fragments arranged by size:
1758 1496 702 258 248 10
BsaWI w'CCGG_w
Cuts at: 1661 2182 2860 3134 3281 3692 1661
Size: 521 678 274 147 411 2441
Fragments arranged by size:
2441 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1349 1379 1349
Size: 30 4442
BseYI C'CCAG_C
Cuts at: 655 1897 2655 3232 3728 655
Size: 1242 758 577 496 1399
Fragments arranged by size:
1399 1242 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2014 2060 3268 104
Size: 1910 46 1208 1308
Fragments arranged by size:
1910 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1486 2264 2454 3246 1486
Size: 778 190 792 2712
Fragments arranged by size:
2712 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1486 2097 2190 2264 2454 2516 3246 1486
Size: 611 93 74 190 62 730 2712
Fragments arranged by size:
2712 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3692 3692
Size: 4472
BspHI T'CATG_A
Cuts at: 599 3648 599
Size: 3049 1423
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1143 1522 1736 2038 2419 1143
Size: 379 214 302 381 3196
Fragments arranged by size:
3196 381 379 302 214
BsrBI CCG'CTC
Cuts at: 50 2764 2818 4302 4467 50
Size: 2714 54 1484 165 55
Fragments arranged by size:
2714 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2384 2384
Size: 4472
BsrFI r'CCGG_y
Cuts at: 2470 2651 2860 2470
Size: 181 209 4082
Fragments arranged by size:
4082 209 181
BsrGI T'GTAC_A
Cuts at: 766 766
Size: 4472
BssSI C'ACGA_G
Cuts at: 2743 3101 2743
Size: 358 4114
BstAPI GCAn_nnn'nTGC
Cuts at: 1849 1849
Size: 4472
BstBI TT'CG_AA
Cuts at: 2833 2833
Size: 4472
BstYI r'GATC_y
Cuts at: 1 798 1254 1311 1497 1964 1987 2322 2568
Size: 797 456 57 186 467 23 335 246
Cuts at: 2568 3580 3666 3678 4024 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 797 467 456 449 346 335 246
186 86 57 23 12
BtgI C'CryG_G
Cuts at: 107 1245 107
Size: 1138 3334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 469 2422 2603 2766 469
Size: 1953 181 163 2175
Fragments arranged by size:
2175 1953 181 163
BtsI GCAGTG_nn'
Cuts at: 1715 1715
Size: 4472
ClaI AT'CG_AT
Cuts at: 1382 1382
Size: 4472
DraI TTT'AAA
Cuts at: 1471 1471
Size: 4472
DrdI GACnn_nn'nnGTC
Cuts at: 2178 3036 2178
Size: 858 3614
EaeI y'GGCC_r
Cuts at: 945 2057 2231 2622 2649 3994 945
Size: 1112 174 391 27 1345 1423
Fragments arranged by size:
1423 1345 1112 391 174 27
EagI C'GGCC_G
Cuts at: 2057 2057
Size: 4472
EarI CTCTTCn'nnn_
Cuts at: 1334 2495 2705 1334
Size: 1161 210 3101
Fragments arranged by size:
3101 1161 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2680 2990 3136 4282 2680
Size: 310 146 1146 2870
Fragments arranged by size:
2870 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1484 1484
Size: 4472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 518 1315 2297 2729 3476 3744 323
Size: 195 797 982 432 747 268 1051
Fragments arranged by size:
1051 982 797 747 432 268 195
EcoO109I rG'GnC_Cy
Cuts at: 1611 1611
Size: 4472
EcoRI G'AATT_C
Cuts at: 259 1476 259
Size: 1217 3255
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 142 631 804 1113 2491 142
Size: 489 173 309 1378 2123
Fragments arranged by size:
2123 1378 489 309 173
FspI TGC'GCA
Cuts at: 2253 2253
Size: 4472
HaeII r_GCGC'y
Cuts at: 138 1906 2154 3176 4155 138
Size: 1768 248 1022 979 455
Fragments arranged by size:
1768 1022 979 455 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 871 903 2314 2344 2346 2376 871
Size: 32 1411 30 2 30 2967
Fragments arranged by size:
2967 1411 32 30 30 2
HincII GTy'rAC
Cuts at: 1511 1511
Size: 4472
KasI G'GCGC_C
Cuts at: 134 1902 2150 134
Size: 1768 248 2456
Fragments arranged by size:
2456 1768 248
KpnI G_GTAC'C
Cuts at: 669 1492 669
Size: 823 3649
MluI A'CGCG_T
Cuts at: 3905 3905
Size: 4472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3143 3327 3143
Size: 184 4288
MscI TGG'CCA
Cuts at: 947 2233 947
Size: 1286 3186
MslI CAynn'nnrTG
Cuts at: 2461 2588 2461
Size: 127 4345
MspA1I CmG'CkG
Cuts at: 1583 1897 2257 3270 3515 1583
Size: 314 360 1013 245 2540
Fragments arranged by size:
2540 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 4472
NarI GG'CG_CC
Cuts at: 135 1903 2151 135
Size: 1768 248 2456
Fragments arranged by size:
2456 1768 248
NcoI C'CATG_G
Cuts at: 107 1245 107
Size: 1138 3334
NdeI CA'TA_TG
Cuts at: 561 561
Size: 4472
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 4472
NspI r_CATG'y
Cuts at: 845 984 1525 2025 2932 845
Size: 139 541 500 907 2385
Fragments arranged by size:
2385 907 541 500 139
PciI A'CATG_T
Cuts at: 2928 2928
Size: 4472
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4472
PfoI T'CCnGG_A
Cuts at: 1052 1052
Size: 4472
PmeI GTTT'AAAC
Cuts at: 1471 1471
Size: 4472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4440
PshAI GACnn'nnGTC
Cuts at: 1030 1030
Size: 4472
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1168 1200 1168
Size: 32 4440
PstI C_TGCA'G
Cuts at: 1140 1452 1519 2204 1140
Size: 312 67 685 3408
Fragments arranged by size:
3408 685 312 67
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4472
PvuII CAG'CTG
Cuts at: 1897 2257 1897
Size: 360 4112
RsrII CG'GwC_CG
Cuts at: 2667 2667
Size: 4472
SacI G_AGCT'C
Cuts at: 1486 1486
Size: 4472
SalI G'TCGA_C
Cuts at: 1509 1509
Size: 4472
SapI GCTCTTCn'nnn_
Cuts at: 2495 2705 2495
Size: 210 4262
SbfI CC_TGCA'GG
Cuts at: 1519 1519
Size: 4472
ScaI AGT'ACT
Cuts at: 952 3989 952
Size: 3037 1435
SfcI C'TryA_G
Cuts at: 33 319 1136 1448 1515 2200 3193 3384 33
Size: 286 817 312 67 685 993 191 1121
Fragments arranged by size:
1121 993 817 685 312 286 191 67
SfoI GGC'GCC
Cuts at: 136 1904 2152 136
Size: 1768 248 2456
Fragments arranged by size:
2456 1768 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
SmaI CCC'GGG
Cuts at: 1494 1494
Size: 4472
SmlI C'TyrA_G
Cuts at: 1627 2542 3034 3296 1627
Size: 915 492 262 2803
Fragments arranged by size:
2803 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4472
SphI G_CATG'C
Cuts at: 845 984 1525 2025 845
Size: 139 541 500 3292
Fragments arranged by size:
3292 541 500 139
SspI AAT'ATT
Cuts at: 220 220
Size: 4472
StyI C'CwwG_G
Cuts at: 107 890 1245 1606 1960 107
Size: 783 355 361 354 2619
Fragments arranged by size:
2619 783 361 355 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2055 2188 2647 2856 2055
Size: 133 459 209 3671
Fragments arranged by size:
3671 459 209 133
TatI w'GTAC_w
Cuts at: 766 950 3987 766
Size: 184 3037 1251
Fragments arranged by size:
3037 1251 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3989 4021 3989
Size: 32 4440
Tth111I GACn'n_nGTC
Cuts at: 1105 2269 1105
Size: 1164 3308
XbaI T'CTAG_A
Cuts at: 1503 1503
Size: 4472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1967 1967
Size: 4472
XmaI C'CCGG_G
Cuts at: 1492 1492
Size: 4472
XmnI GAAnn'nnTTC
Cuts at: 125 570 784 1393 125
Size: 445 214 609 3204
Fragments arranged by size:
3204 609 445 214
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PshAI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BseRI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI