Restriction map for pF1KB7775
 (Linear) (Six-base) MAPSORT of: KIBB7775.seq  Check: 158  from: 1  to: 1359



KIBB7775.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

Acc65I G'GTAC_C

Cuts at:      0     557    1359 
   Size:        557     802
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     215    1207    1359 
   Size:        215     992     152

  Fragments arranged by size: 

                 992     215     152
 
ApoI r'AATT_y

Cuts at:      0     151     461     716    1359 
   Size:        151     310     255     643

  Fragments arranged by size: 

                 643     310     255     151
 
BamHI G'GATC_C

Cuts at:      0    1203    1359 
   Size:       1203     156
 
BanI G'GyrC_C

Cuts at:      0      26     557    1359 
   Size:         26     531     802

  Fragments arranged by size: 

                 802     531      26
 
BbeI G_GCGC'C

Cuts at:      0      30    1359 
   Size:         30    1329
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     918    1359 
   Size:        918     441
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1069    1359 
   Size:       1069     290
 
BclI T'GATC_A

Cuts at:      0     350    1359 
   Size:        350    1009
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410    1359 
   Size:        410     949
 
Bpu10I CC'TnA_GC

Cuts at:      0     763    1359 
   Size:        763     596
 
BsaBI GATnn'nnATC

Cuts at:      0     860    1359 
   Size:        860     499
 
BsaHI Gr'CG_yC

Cuts at:      0      27      37    1359 
   Size:         27      10    1322

  Fragments arranged by size: 

                1322      27      10
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1241    1271    1359 
   Size:       1241      30      88

  Fragments arranged by size: 

                1241      88      30
 
BseYI C'CCAG_C

Cuts at:      0     547    1359 
   Size:        547     812
 
BspHI T'CATG_A

Cuts at:      0     491    1359 
   Size:        491     868
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1035    1359 
   Size:       1035     324
 
BsrGI T'GTAC_A

Cuts at:      0     658    1359 
   Size:        658     701
 
BstYI r'GATC_y

Cuts at:      0     690    1146    1203    1359 
   Size:        690     456      57     156

  Fragments arranged by size: 

                 690     456     156      57
 
BtgI C'CryG_G

Cuts at:      0    1137    1359 
   Size:       1137     222
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     361    1359 
   Size:        361     998
 
ClaI AT'CG_AT

Cuts at:      0    1274    1359 
   Size:       1274      85
 
EaeI y'GGCC_r

Cuts at:      0     837    1359 
   Size:        837     522
 
EarI CTCTTCn'nnn_

Cuts at:      0    1226    1359 
   Size:       1226     133
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     410    1207    1359 
   Size:        215     195     797     152

  Fragments arranged by size: 

                 797     215     195     152
 
EcoRI G'AATT_C

Cuts at:      0     151    1359 
   Size:        151    1208
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      34     523     696    1005    1359 
   Size:         34     489     173     309     354

  Fragments arranged by size: 

                 489     354     309     173      34
 
HaeII r_GCGC'y

Cuts at:      0      30    1359 
   Size:         30    1329
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     763     795    1359 
   Size:        763      32     564

  Fragments arranged by size: 

                 763     564      32
 
KasI G'GCGC_C

Cuts at:      0      26    1359 
   Size:         26    1333
 
KpnI G_GTAC'C

Cuts at:      0     561    1359 
   Size:        561     798
 
MscI TGG'CCA

Cuts at:      0     839    1359 
   Size:        839     520
 
NarI GG'CG_CC

Cuts at:      0      27    1359 
   Size:         27    1332
 
NcoI C'CATG_G

Cuts at:      0    1137    1359 
   Size:       1137     222
 
NdeI CA'TA_TG

Cuts at:      0     453    1359 
   Size:        453     906
 
NspI r_CATG'y

Cuts at:      0     737     876    1359 
   Size:        737     139     483

  Fragments arranged by size: 

                 737     483     139
 
PfoI T'CCnGG_A

Cuts at:      0     944    1359 
   Size:        944     415
 
PshAI GACnn'nnGTC

Cuts at:      0     922    1359 
   Size:        922     437
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1060    1092    1359 
   Size:       1060      32     267

  Fragments arranged by size: 

                1060     267      32
 
PstI C_TGCA'G

Cuts at:      0    1032    1344    1359 
   Size:       1032     312      15

  Fragments arranged by size: 

                1032     312      15
 
ScaI AGT'ACT

Cuts at:      0     844    1359 
   Size:        844     515
 
SfcI C'TryA_G

Cuts at:      0     211    1028    1340    1359 
   Size:        211     817     312      19

  Fragments arranged by size: 

                 817     312     211      19
 
SfoI GGC'GCC

Cuts at:      0      28    1359 
   Size:         28    1331
 
SphI G_CATG'C

Cuts at:      0     737     876    1359 
   Size:        737     139     483

  Fragments arranged by size: 

                 737     483     139
 
SspI AAT'ATT

Cuts at:      0     112    1359 
   Size:        112    1247
 
StyI C'CwwG_G

Cuts at:      0     782    1137    1359 
   Size:        782     355     222

  Fragments arranged by size: 

                 782     355     222
 
TatI w'GTAC_w

Cuts at:      0     658     842    1359 
   Size:        658     184     517

  Fragments arranged by size: 

                 658     517     184
 
Tth111I GACn'n_nGTC

Cuts at:      0     997    1359 
   Size:        997     362
 
XmnI GAAnn'nnTTC

Cuts at:      0      17     462     676    1285    1359 
   Size:         17     445     214     609      74

  Fragments arranged by size: 

                 609     445     214      74      17
 

 Enzymes that do cut:

   Acc65I     AcuI     ApoI    BamHI     BanI     BbeI     BbsI    BciVI
     BclI     BpmI   Bpu10I    BsaBI    BsaHI    BsaXI    BseYI    BspHI
    BspMI    BsrGI    BstYI     BtgI    BtgZI     ClaI     EaeI     EarI
  Eco57MI    EcoRI     FalI    HaeII    Hin4I     KasI     KpnI     MscI
     NarI     NcoI     NdeI     NspI     PfoI    PshAI     PsrI     PstI
     ScaI     SfcI     SfoI     SphI     SspI     StyI     TatI  Tth111I
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BanII    BbvCI     BcgI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaWI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtsI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     StuI     SwaI    TaqII     TstI     XbaI     XcmI
     XhoI     XmaI     ZraI
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