Restriction map for pF1KE0172
 (Linear) (Six-base) MAPSORT of: KIEE0172.seq  Check: 9062  from: 1  to: 951



KIEE0172.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AatII G_ACGT'C

Cuts at:      0     841     951 
   Size:        841     110
 
AccI GT'mk_AC

Cuts at:      0     596     951 
   Size:        596     355
 
AflII C'TTAA_G

Cuts at:      0     435     951 
   Size:        435     516
 
AlwNI CAG_nnn'CTG

Cuts at:      0     514     951 
   Size:        514     437
 
ApaI G_GGCC'C

Cuts at:      0     124     951 
   Size:        124     827
 
ApoI r'AATT_y

Cuts at:      0     133     951 
   Size:        133     818
 
BanI G'GyrC_C

Cuts at:      0     810     951 
   Size:        810     141
 
BanII G_rGCy'C

Cuts at:      0     124     899     951 
   Size:        124     775      52

  Fragments arranged by size: 

                 775     124      52
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     291     951 
   Size:        291     660
 
BbvCI CC'TCA_GC

Cuts at:      0     416     951 
   Size:        416     535
 
Bme1580I G_kGCm'C

Cuts at:      0     124     951 
   Size:        124     827
 
BmgBI CAC'GTC

Cuts at:      0      37     951 
   Size:         37     914
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     501     951 
   Size:        501     450
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     951 
   Size:        496     455
 
Bpu10I CC'TnA_GC

Cuts at:      0     416     951 
   Size:        416     535
 
BsaBI GATnn'nnATC

Cuts at:      0     559     951 
   Size:        559     392
 
BsaHI Gr'CG_yC

Cuts at:      0     838     951 
   Size:        838     113
 
BsaWI w'CCGG_w

Cuts at:      0     443     951 
   Size:        443     508
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     357     403     951 
   Size:        357      46     548

  Fragments arranged by size: 

                 548     357      46
 
BseYI C'CCAG_C

Cuts at:      0     917     951 
   Size:        917      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     817     951 
   Size:        817     134
 
BsiHKAI G_wGCw'C

Cuts at:      0     229     951 
   Size:        229     722
 
BsiWI C'GTAC_G

Cuts at:      0     125     951 
   Size:        125     826
 
BsmI GAATG_Cn'

Cuts at:      0     826     951 
   Size:        826     125
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     845     951 
   Size:        845     106
 
Bsp1286I G_dGCh'C

Cuts at:      0     124     229     899     951 
   Size:        124     105     670      52

  Fragments arranged by size: 

                 670     124     105      52
 
BspEI T'CCGG_A

Cuts at:      0     443     951 
   Size:        443     508
 
BssSI C'ACGA_G

Cuts at:      0     385     951 
   Size:        385     566
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     721     951 
   Size:        721     230
 
BstYI r'GATC_y

Cuts at:      0      30     330     554     951 
   Size:         30     300     224     397

  Fragments arranged by size: 

                 397     300     224      30
 
BtgI C'CryG_G

Cuts at:      0      62     951 
   Size:         62     889
 
BtsI GCAGTG_nn'

Cuts at:      0     284     951 
   Size:        284     667
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      23      58     951 
   Size:         23      35     893

  Fragments arranged by size: 

                 893      35      23
 
EaeI y'GGCC_r

Cuts at:      0      71     951 
   Size:         71     880
 
EarI CTCTTCn'nnn_

Cuts at:      0     103     904     951 
   Size:        103     801      47

  Fragments arranged by size: 

                 801     103      47
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     951 
   Size:        496     455
 
EcoO109I rG'GnC_Cy

Cuts at:      0     273     951 
   Size:        273     678
 
EcoRV GAT'ATC

Cuts at:      0     363     951 
   Size:        363     588
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     352     384     631     663     844     876     951 
   Size:        352      32     247      32     181      32      75

  Fragments arranged by size: 

                 352     247     181      75      32      32      32
 
HincII GTy'rAC

Cuts at:      0     863     951 
   Size:        863      88
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     329     703     951 
   Size:        329     374     248

  Fragments arranged by size: 

                 374     329     248
 
MscI TGG'CCA

Cuts at:      0      73     951 
   Size:         73     878
 
MslI CAynn'nnrTG

Cuts at:      0      13      61     719     951 
   Size:         13      48     658     232

  Fragments arranged by size: 

                 658     232      48      13
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     925     951 
   Size:        925      26
 
PspOMI G'GGCC_C

Cuts at:      0     120     951 
   Size:        120     831
 
PstI C_TGCA'G

Cuts at:      0     338     951 
   Size:        338     613
 
SapI GCTCTTCn'nnn_

Cuts at:      0     904     951 
   Size:        904      47
 
SfcI C'TryA_G

Cuts at:      0     334     951 
   Size:        334     617
 
SmlI C'TyrA_G

Cuts at:      0     435     951 
   Size:        435     516
 
StyI C'CwwG_G

Cuts at:      0     269     951 
   Size:        269     682
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      42     951 
   Size:         42     909
 
Tth111I GACn'n_nGTC

Cuts at:      0     601     951 
   Size:        601     350
 
XmnI GAAnn'nnTTC

Cuts at:      0     112     951 
   Size:        112     839
 
ZraI GAC'GTC

Cuts at:      0     839     951 
   Size:        839     112
 

 Enzymes that do cut:

    AatII     AccI    AflII    AlwNI     ApaI     ApoI     BanI    BanII
     BbsI    BbvCI Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BssSI    BstXI    BstYI     BtgI     BtsI
    CspCI     EaeI     EarI  Eco57MI EcoO109I    EcoRV    Hin4I   HincII
     MmeI     MscI     MslI     PpiI   PspOMI     PstI     SapI     SfcI
     SmlI     StyI    TaqII  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmrI     BmtI    BpuEI     BsaI    BsaAI
    BsaXI    BsiEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI     XbaI     XcmI     XhoI     XmaI
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