Restriction map for pF1KB7064
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       BbeI        
BbrPI       BciVI       BclI        BfrI        BglII       
BsaAI       BsaBI       BsaI        BsgI        BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspLU11I    BspMI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     ClaI        CpoI        DraI        
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoT22I     FbaI        FspI        HindIII     
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccII          91  CGCG                 CG^CG
AccIII       1632  TCCGGA               T^CCGGA
AfaI         1537  GTAC                 GT^AC
AhdI          766  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         766  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         416  GTGCTC               G[AT]GC[AT]^C
AvrII        1573  CCTAGG               C^CTAGG
BamHI         640  GGATCC               G^GATCC
BanI          908  GGTGCC               G^G[CT][GA]CC
BglI          104  GCCTGAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1573  CCTAGG               C^CTAGG
BsaWI        1632  TCCGGA               [AT]^CCGG[AT]
BseAI        1632  TCCGGA               T^CCGGA
BsiHKAI       416  GTGCTC               G[AT]GC[AT]^C
BspEI        1632  TCCGGA               T^CCGGA
BspHI        1626  TCATGA               T^CATGA
BsrBI        1971  CCGCTC               CCGCTC(-3/-3)
BsrFI        1716  GCCGGC               [GA]^CCGG[CT]
BstAPI       1987  GCAGCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          91  CGCG                 CG^CG
BstYI         640  GGATCC               [GA]^GATC[CT]
Cfr10I       1716  GCCGGC               [GA]^CCGG[CT]
DraIII       1001  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          735  GACCTCCGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      766  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1138  CCTGCTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1448  GATATC               GAT^ATC
FseI         1715  GGCCGGCC             GGCCGG^CC
HaeII        1607  AGCGCC               [GA]GCGC^[CT]
HincII        520  GTTAAC               GT[CT]^[GA]AC
HindII        520  GTTAAC               GT[CT]^[GA]AC
HpaI          520  GTTAAC               GTT^AAC
MflI          640  GGATCC               [GA]^GATC[CT]
MroI         1632  TCCGGA               T^CCGGA
MseI          521  TTAA                 T^TAA
MslI          654  CACTTGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           91  CGCG                 CG^CG
NaeI         1716  GCCGGC               GCC^GGC
NgoMIV       1716  GCCGGC               G^CCGGC
NspI          347  GCATGT               [GA]CATG^[CT]
PstI         1985  CTGCAG               CTGCA^G
PvuII         956  CAGCTG               CAG^CTG
RcaI         1626  TCATGA               T^CATGA
RsaI         1537  GTAC                 GT^AC
SmaI         1832  CCCGGG               CCC^GGG
TaqI         1452  TCGA                 T^CGA
Tru9I         521  TTAA                 T^TAA
Tsp509I       398  AATT                 ^AATT
TthHB8I      1452  TCGA                 T^CGA
XbaI          123  TCTAGA               T^CTAGA
XcmI         1552  CCACCGCCTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         640  GGATCC               [GA]^GATC[CT]
XmaCI        1832  CCCGGG               C^CCGGG
XmaI         1832  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          230  GACGCC               G[GA]^CG[CT]C
              798  GGCGTC              
AocI          105  CCTGAGG              CC^T[ACGT]AGG
             1854  CCTCAGG             
ApaI         1427  GGGCCC               GGGCC^C
             1737  GGGCCC              
AvaI         1757  CTCGGG               C^[CT]CG[GA]G
             1832  CCCGGG              
BcgI         1749  GCAGCTTGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1766  GCAGCCGCTTCG        
BlpI         1504  GCTGAGC              GC^T[ACGT]AGC
             1806  GCTGAGC             
Bpu1102I     1504  GCTGAGC              GC^T[ACGT]AGC
             1806  GCTGAGC             
BsaHI         230  GACGCC               G[GA]^CG[CT]C
              798  GGCGTC              
BsoBI        1757  CTCGGG               C^[CT]CG[GA]G
             1832  CCCGGG              
Bsu36I        105  CCTGAGG              CC^T[ACGT]AGG
             1854  CCTCAGG             
CelII        1504  GCTGAGC              GC^T[ACGT]AGC
             1806  GCTGAGC             
Eco81I        105  CCTGAGG              CC^T[ACGT]AGG
             1854  CCTCAGG             
FauI         1730  CCCGC                CCCGC(4/6)
             1970  CCCGC               
HgaI          230  GACGC                GACGC(5/10)
              799  GCGTC               
Hin1I         230  GACGCC               G[GA]^CG[CT]C
              798  GGCGTC              
MaeIII       1085  GTGAC                ^GT[ACGT]AC
             1684  GTCAC               
MspA1I        956  CAGCTG               C[AC]G^C[GT]G
             1988  CAGCGG              
NlaIII        348  CATG                 CATG^
             1627  CATG                
PpuMI         117  GGGACCT              [GA]G^G[AT]CC[CT]
              772  AGGTCCT             
SfcI         1142  CTACAG               C^T[GA][CT]AG
             1985  CTGCAG              
StuI         1019  AGGCCT               AGG^CCT
             1289  AGGCCT              
TfiI          684  GAATC                G^A[AT]TC
             1366  GATTC               
Tsp45I       1085  GTGAC                ^GT[GC]AC
             1684  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AvaII       
BalI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MboI        MboII       MluNI       MnlI        MscI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIV       PflMI       PleI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        StyI        TseI        
TspRI       Van91I      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com