Restriction map for pF1KE0130
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AfaI        AflII       AflIII      AgeI        Aor51HI     
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AvrII       BamHI       BbrPI       BbsI        
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BsrGI       BssHII      Bst1107I    BstBI       
BstYI       BstZ17I     ClaI        CpoI        DraI        
DraIII      EagI        EarI        EclXI       Eco47III    
Eco52I      EcoRV       EcoT22I     FbaI        FseI        
HindIII     HpaI        KpnI        Ksp632I     MamI        
MboII       MfeI        MflI        MluI        MroI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tru9I       Tth111I     
XbaI        XhoII       XmnI        


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# ONE CUT ENZYMES (   64 enzymes) #
###################################

AcsI          100  GAATTC               [GA]^AATT[CT]
AcyI          370  GGCGCC               G[GA]^CG[CT]C
AhdI          700  GACAACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        625  GTGCAC               G^TGCAC
AlwI          410  GATCC                GGATC(4/5)
AlwNI         457  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         625  GTGCAC               G^TGCAC
ApoI          100  GAATTC               [GA]^AATT[CT]
AspEI         700  GACAACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         389  TGCGCA               TGC^GCA
BalI          827  TGGCCA               TGG^CCA
BbeI          370  GGCGCC               GGCGC^C
BbvCI        1144  GCTGAGG              CCTCAGC(-5/-2)
BlpI          742  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      742  GCTCAGC              GC^T[ACGT]AGC
BsaHI         370  GGCGCC               G[GA]^CG[CT]C
BsmBI         763  CGTCTC               CGTCTC(1/5)
BsrBI         740  CCGCTC               CCGCTC(-3/-3)
BssSI         622  CTCGTG               CACGAG(-5/-1)
BstEII          6  GGTGACC              G^GT[ACGT]ACC
BstPI           6  GGTGACC              G^GT[ACGT]ACC
BstXI         687  CCAGGTGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         742  GCTCAGC              GC^T[ACGT]AGC
DpnII         410  GATC                 ^GATC
DrdI          208  GACCGGGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      700  GACAACGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I         6  GGTGACC              G^GT[ACGT]ACC
EcoRI         100  GAATTC               G^AATTC
EcoT14I       809  CCATGG               C^C[AT][AT]GG
FauI          649  CCCGC                CCCGC(4/6)
FspI          389  TGCGCA               TGC^GCA
HaeII         370  GGCGCC               [GA]GCGC^[CT]
HgaI          762  GCGTC                GACGC(5/10)
Hin1I         370  GGCGCC               G[GA]^CG[CT]C
KasI          370  GGCGCC               G^GCGCC
KspI          188  CCGCGG               CCGC^GG
MaeII         269  ACGT                 A^CGT
MaeIII          7  GTGAC                ^GT[ACGT]AC
MboI          410  GATC                 ^GATC
MluNI         827  TGGCCA               TGG^CCA
MscI          827  TGGCCA               TGG^CCA
MspA1I        188  CCGCGG               C[AC]G^C[GT]G
NaeI          367  GCCGGC               GCC^GGC
NarI          370  GGCGCC               GG^CGCC
NcoI          809  CCATGG               C^CATGG
NdeII         410  GATC                 ^GATC
NgoMIV        367  GCCGGC               G^CCGGC
NspI          546  GCATGT               [GA]CATG^[CT]
PaeR7I        115  CTCGAG               C^TCGAG
PflMI         456  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1095  AGGTCCT              [GA]G^G[AT]CC[CT]
SacII         188  CCGCGG               CCGC^GG
Sau3AI        410  GATC                 ^GATC
SfoI          370  GGCGCC               GGC^GCC
SmaI         1037  CCCGGG               CCC^GGG
StuI          130  AGGCCT               AGG^CCT
StyI          809  CCATGG               C^C[AT][AT]GG
Tsp45I          7  GTGAC                ^GT[GC]AC
Tsp509I       101  AATT                 ^AATT
Van91I        456  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          185  CCACCGCGGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          115  CTCGAG               C^TCGAG
XmaCI        1037  CCCGGG               C^CCGGG
XmaI         1037  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AocI          126  CCTGAGG              CC^T[ACGT]AGG
              855  CCTGAGG             
ApaI          258  GGGCCC               GGGCC^C
              875  GGGCCC              
AvaI          115  CTCGAG               C^[CT]CG[GA]G
             1037  CCCGGG              
BglI          184  GCCACCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              652  GCCAGGACGGC         
BsoBI         115  CTCGAG               C^[CT]CG[GA]G
             1037  CCCGGG              
BstAPI        392  GCAGGGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              618  GCAGCTCGTGC         
Bsu36I        126  CCTGAGG              CC^T[ACGT]AGG
              855  CCTGAGG             
Eco81I        126  CCTGAGG              CC^T[ACGT]AGG
              855  CCTGAGG             
HincII         43  GTCAAC               GT[CT]^[GA]AC
              217  GTCAAC              
HindII         43  GTCAAC               GT[CT]^[GA]AC
              217  GTCAAC              
MslI          534  CACCGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              720  CATCCGGGTG          
SacI          202  GAGCTC               GAGCT^C
             1150  GAGCTC              
SmlI          115  CTCGAG               C^T[CT][GA]AG
             1168  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaI        BsaJI       BseRI       
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DraII       DsaI        
EaeI        EcoNI       EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MnlI        MspI        MvaI        MvnI        MwoI        
NciI        NlaIII      NlaIV       PleI        PstI        
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TseI        TspRI       TthHB8I     
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