Restriction map for pF1KE0130
 (Linear) (Six-base) MAPSORT of: KIEE0130.seq  Check: 317  from: 1  to: 1197



KIEE0130.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     705    1197 
   Size:        705     492
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      76    1197 
   Size:         76    1121
 
AlwNI CAG_nnn'CTG

Cuts at:      0     462    1197 
   Size:        462     735
 
ApaI G_GGCC'C

Cuts at:      0     262     879    1197 
   Size:        262     617     318

  Fragments arranged by size: 

                 617     318     262
 
ApaLI G'TGCA_C

Cuts at:      0     625    1197 
   Size:        625     572
 
ApoI r'AATT_y

Cuts at:      0     100    1197 
   Size:        100    1097
 
AvaI C'yCGr_G

Cuts at:      0     115    1037    1197 
   Size:        115     922     160

  Fragments arranged by size: 

                 922     160     115
 
BanI G'GyrC_C

Cuts at:      0      26     370     452    1033    1045    1197 
   Size:         26     344      82     581      12     152

  Fragments arranged by size: 

                 581     344     152      82      26      12
 
BanII G_rGCy'C

Cuts at:      0     206     262     879     962    1014    1154    1169    1197 
   Size:        206      56     617      83      52     140      15      28

  Fragments arranged by size: 

                 617     206     140      83      56      52      28      15
            
 
BbeI G_GCGC'C

Cuts at:      0     374    1197 
   Size:        374     823
 
BbvCI CC'TCA_GC

Cuts at:      0    1145    1197 
   Size:       1145      52
 
BglI GCCn_nnn'nGGC

Cuts at:      0     190     658    1197 
   Size:        190     468     539

  Fragments arranged by size: 

                 539     468     190
 
BlpI GC'TnA_GC

Cuts at:      0     743    1197 
   Size:        743     454
 
Bme1580I G_kGCm'C

Cuts at:      0     262     284     629     879    1038    1050    1197 
   Size:        262      22     345     250     159      12     147

  Fragments arranged by size: 

                 345     262     250     159     147      22      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     263    1197 
   Size:        263     934
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     783    1197 
   Size:        783     414
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1108    1141    1197 
   Size:       1108      33      56

  Fragments arranged by size: 

                1108      56      33
 
Bpu10I CC'TnA_GC

Cuts at:      0     291    1145    1197 
   Size:        291     854      52

  Fragments arranged by size: 

                 854     291      52
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1153    1197 
   Size:       1153      44
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     316     567     938    1197 
   Size:        316     251     371     259

  Fragments arranged by size: 

                 371     316     259     251
 
BsaHI Gr'CG_yC

Cuts at:      0     371    1197 
   Size:        371     826
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     187     313     517     591     594     597    1111    1144 
   Size:        187     126     204      74       3       3     514      33

Cuts at:   1144    1162    1197 
   Size:         18      35

  Fragments arranged by size: 

                 514     204     187     126      74      35      33      18
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0     954    1197 
   Size:        954     243
 
BsiEI CG_ry'CG

Cuts at:      0     210     318     846    1197 
   Size:        210     108     528     351

  Fragments arranged by size: 

                 528     351     210     108
 
BsiHKAI G_wGCw'C

Cuts at:      0     206     629    1154    1197 
   Size:        206     423     525      43

  Fragments arranged by size: 

                 525     423     206      43
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     769    1197 
   Size:        769     428
 
Bsp1286I G_dGCh'C

Cuts at:      0     206     262     284     629     879     962    1014    1038 
   Size:        206      56      22     345     250      83      52      24

Cuts at:   1038    1050    1154    1169    1197 
   Size:         12     104      15      28

  Fragments arranged by size: 

                 345     250     206     104      83      56      52      28
                  24      22      15      12
 
BsrBI CCG'CTC

Cuts at:      0     742    1197 
   Size:        742     455
 
BsrFI r'CCGG_y

Cuts at:      0      29     367    1030    1197 
   Size:         29     338     663     167

  Fragments arranged by size: 

                 663     338     167      29
 
BssSI C'ACGA_G

Cuts at:      0     622    1197 
   Size:        622     575
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     398     624    1197 
   Size:        398     226     573

  Fragments arranged by size: 

                 573     398     226
 
BstEII G'GTnAC_C

Cuts at:      0       6    1197 
   Size:          6    1191
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     694    1197 
   Size:        694     503
 
Bsu36I CC'TnA_GG

Cuts at:      0     127     856    1197 
   Size:        127     729     341

  Fragments arranged by size: 

                 729     341     127
 
BtgI C'CryG_G

Cuts at:      0     188     262     809    1197 
   Size:        188      74     547     388

  Fragments arranged by size: 

                 547     388     188      74
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     214    1197 
   Size:        214     983
 
EaeI y'GGCC_r

Cuts at:      0     365     827     923    1197 
   Size:        365     462      96     274

  Fragments arranged by size: 

                 462     365     274      96
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     655     828    1197 
   Size:        655     173     369

  Fragments arranged by size: 

                 655     369     173
 
EcoICRI GAG'CTC

Cuts at:      0     204    1152    1197 
   Size:        204     948      45

  Fragments arranged by size: 

                 948     204      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1108    1141    1197 
   Size:       1108      33      56

  Fragments arranged by size: 

                1108      56      33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     685    1123    1159    1197 
   Size:        685     438      36      38

  Fragments arranged by size: 

                 685     438      38      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0      57     258     822     875    1096    1116    1197 
   Size:         57     201     564      53     221      20      81

  Fragments arranged by size: 

                 564     221     201      81      57      53      20
 
EcoRI G'AATT_C

Cuts at:      0     100    1197 
   Size:        100    1097
 
FspI TGC'GCA

Cuts at:      0     391    1197 
   Size:        391     806
 
HaeII r_GCGC'y

Cuts at:      0     374    1197 
   Size:        374     823
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     691     723    1197 
   Size:        691      32     474

  Fragments arranged by size: 

                 691     474      32
 
HincII GTy'rAC

Cuts at:      0      45     219    1197 
   Size:         45     174     978

  Fragments arranged by size: 

                 978     174      45
 
KasI G'GCGC_C

Cuts at:      0     370    1197 
   Size:        370     827
 
MscI TGG'CCA

Cuts at:      0     829    1197 
   Size:        829     368
 
MslI CAynn'nnrTG

Cuts at:      0     538     724    1197 
   Size:        538     186     473

  Fragments arranged by size: 

                 538     473     186
 
MspA1I CmG'CkG

Cuts at:      0     190    1197 
   Size:        190    1007
 
NaeI GCC'GGC

Cuts at:      0     369    1197 
   Size:        369     828
 
NarI GG'CG_CC

Cuts at:      0     371    1197 
   Size:        371     826
 
NcoI C'CATG_G

Cuts at:      0     809    1197 
   Size:        809     388
 
NgoMIV G'CCGG_C

Cuts at:      0     367    1197 
   Size:        367     830
 
NspI r_CATG'y

Cuts at:      0     550    1197 
   Size:        550     647
 
PasI CC'CwG_GG

Cuts at:      0     236     375     434     496    1197 
   Size:        236     139      59      62     701

  Fragments arranged by size: 

                 701     236     139      62      59
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     462    1197 
   Size:        462     735
 
PfoI T'CCnGG_A

Cuts at:      0      77     332     475    1157    1178    1197 
   Size:         77     255     143     682      21      19

  Fragments arranged by size: 

                 682     255     143      77      21      19
 
PpuMI rG'GwC_Cy

Cuts at:      0    1096    1197 
   Size:       1096     101
 
PspOMI G'GGCC_C

Cuts at:      0     258     875    1197 
   Size:        258     617     322

  Fragments arranged by size: 

                 617     322     258
 
PspXI vC'TCGA_Gb

Cuts at:      0     115    1197 
   Size:        115    1082
 
PstI C_TGCA'G

Cuts at:      0      20     620     803    1197 
   Size:         20     600     183     394

  Fragments arranged by size: 

                 600     394     183      20
 
SacI G_AGCT'C

Cuts at:      0     206    1154    1197 
   Size:        206     948      43

  Fragments arranged by size: 

                 948     206      43
 
SacII CC_GC'GG

Cuts at:      0     191    1197 
   Size:        191    1006
 
SfcI C'TryA_G

Cuts at:      0      16     616     799    1197 
   Size:         16     600     183     398

  Fragments arranged by size: 

                 600     398     183      16
 
SfoI GGC'GCC

Cuts at:      0     372    1197 
   Size:        372     825
 
SmaI CCC'GGG

Cuts at:      0    1039    1197 
   Size:       1039     158
 
SmlI C'TyrA_G

Cuts at:      0     115    1168    1197 
   Size:        115    1053      29

  Fragments arranged by size: 

                1053     115      29
 
SrfI GCCC'GGGC

Cuts at:      0    1039    1197 
   Size:       1039     158
 
StuI AGG'CCT

Cuts at:      0     132    1197 
   Size:        132    1065
 
StyI C'CwwG_G

Cuts at:      0     809    1197 
   Size:        809     388
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     298     304     367     406     426    1197 
   Size:        298       6      63      39      20     771

  Fragments arranged by size: 

                 771     298      63      39      20       6
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     192    1197 
   Size:        192    1005
 
XhoI C'TCGA_G

Cuts at:      0     115    1197 
   Size:        115    1082
 
XmaI C'CCGG_G

Cuts at:      0    1037    1197 
   Size:       1037     160
 

 Enzymes that do cut:

     AhdI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI    BbvCI     BglI     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BseRI    BseYI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BsrBI    BsrFI    BssSI   BstAPI   BstEII
    BstXI   Bsu36I     BtgI     DrdI     EaeI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII     KasI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI    PpuMI   PspOMI    PspXI     PstI     SacI
    SacII     SfcI     SfoI     SmaI     SmlI     SrfI     StuI     StyI
    TaqII     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AloI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmtI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII    BstBI
    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EagI     EarI    EcoRV     FalI     FseI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
     PsrI     PvuI    PvuII    RsrII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XmnI     ZraI
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